|本期目录/Table of Contents|

[1]刘红彦,马国兴,杨力媛,等. 西兰花端粒酶分离与活性测定探究[J].浙江理工大学学报,2012,29(04):604-608.
 LIU Hong yan,MA Guo xing,YANG Li yuan,et al. Isolation and Activity Evaluation of Broccoli Telomerase[J].Journal of Zhejiang Sci-Tech University,2012,29(04):604-608.
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 西兰花端粒酶分离与活性测定探究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年04期
页码:
604-608
栏目:
生物与生命科学
出版日期:
2012-08-28

文章信息/Info

Title:
 Isolation and Activity Evaluation of Broccoli Telomerase
文章编号:
16733851 (2012) 04060405
作者:
 刘红彦 马国兴 杨力媛 郑洁 刘小川
 浙江理工大学生物工程研究所, 杭州 310018
Author(s):
 LIU Hongyan MA Guoxing YANG Liyuan ZHENG Jie LIU Xiaochuan
 Bioengineering Institute, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 西兰花 端粒酶分离 端粒酶检测 聚乙二醇(PEG)
分类号:
Q814.1
文献标志码:
A
摘要:
     植物端粒酶以自身的RNA亚基为模板,维持着染色体末端端粒的长度,从而使植物细胞可持续分裂。本研究以西兰花花蕾为材料,采用不同分子量、不同浓度的PEG分别提取端粒酶,根据PEG吸附蛋白质的原理,从具有端粒酶活性的组织中分离并富集端粒酶。结果显示:用PEG 6000提取西兰花端粒酶,且用100 μg的酶量进行酶促反应时测得的端粒酶活性最高。以此为参数,经改进的TRAP法成功检测到:用0.2 g PEG 6000/700 μL洗脱液提取出的端粒酶活性很高。这些分离、检测技术为其它植物组织中端粒酶活性的检测和评价提供了新的途径,尤其对低活性的植物端粒酶检测具有重要意义,为深入研究端粒酶RNA的序列及结构奠定了基础。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2011-12-21
资金项目: 浙江省研究生创新基金(YK2010057)
作者简介: 刘红彦(1986-),女,山西霍州市人,硕士研究生,研究方向为端粒与端粒酶。
通讯作者: 刘小川,电子信箱:xcliu@zstu.edu.cn
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