|本期目录/Table of Contents|

[1]马琴琴,严国权,郭江峰,等. 影响RNA芯片探针杂交效率的因素分析[J].浙江理工大学学报,2012,29(04):599-603.
 MA Qin qin,YAN Guo quan,GUO Jiang feng,et al. Factors Analysis of Oligodeoxyribonucleotide Probes for RNA Microarray[J].Journal of Zhejiang Sci-Tech University,2012,29(04):599-603.
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 影响RNA芯片探针杂交效率的因素分析()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年04期
页码:
599-603
栏目:
生物与生命科学
出版日期:
2012-08-28

文章信息/Info

Title:
 Factors Analysis of Oligodeoxyribonucleotide Probes for RNA Microarray
文章编号:
16733851 (2012) 04059905
作者:
 马琴琴 严国权 郭江峰 丁先锋
 浙江理工大学生命科学学院生物工程研究所, 杭州 310018
Author(s):
 MA Qinqin YAN Guoquan GUO Jiangfeng DING Xianfeng
 Institute of Bioengineering, School of Life Sciences, Zhejiang SciTech University,Hangzhou 310018, China
关键词:
 覆瓦式探针 RNA芯片 二级结构 探针/靶序列杂交双链
分类号:
Q789
文献标志码:
A
摘要:
     RNA芯片作为研究ncRNA的主要技术之一,已受到广泛关注。然而因RNA芯片中靶序列的单链特征,使得RNA芯片的探针设计比DNA复杂。本研究对加强型绿色荧光蛋白(EGFP)RNA序列进行覆瓦式探针设计,〖JP2〗制作了RNA原位合成芯片,研究探针/靶序列杂交双链ΔG和Tm、靶序列二级结构以及探针的末端碱基对RNA芯片杂交信号强度的影响。结果表明,探针/靶序列杂交双链ΔG和Tm相同的探针间的杂交信号存在较大差异,探针/靶序列杂交双链ΔG和Tm与RNA芯片杂交信号之间未发现明显的规律性;靶序列二级结构的探针PTsc参数与芯片杂交信号强度间呈较好的线性关系;探针5’末端碱基组成对芯片杂交信号强度也有影响,5’末端碱基为G/C的探针杂交信号总体上高于其邻近的5’末端碱基为A/T的探针的杂交信号,为RNA芯片的探针筛选提供一定的理论基础

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备注/Memo

备注/Memo:
 收稿日期: 2011-11-22
基金项目: 国家高技术研究发展计划项目(863计划,2007AA02Z165);广东省教育部产学研结合项目(2011B090400478);浙江省自然科学基金项目(Y2100681);杭州市科技发展计划项目(20110733Q04,20110733Q21)
作者简介: 马琴琴(1986-),女,湖北荆州人,硕士研究生,从事生物化学与分子生物学研究。
通讯作者: 丁先锋,Email:xfding@zstu.edu.cn
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