|本期目录/Table of Contents|

[1]吕赛群,金华君,钱其军. miR29b对肝癌细胞中甲基化转移酶表达的调控作用[J].浙江理工大学学报,2011,28(01):101-105.
 L Sai qun,JIN Hua jun,QIAN Qi jun. Regulating the Expression of DNA Methyltransferase in Liver Cancer Cells by miR29b[J].Journal of Zhejiang Sci-Tech University,2011,28(01):101-105.
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 miR29b对肝癌细胞中甲基化转移酶表达的调控作用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
101-105
栏目:
生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Regulating the Expression of DNA Methyltransferase in Liver Cancer Cells by miR29b
文章编号:
16733851 (2011) 01010105
作者:
 吕赛群1 金华君2 钱其军12
 1. 浙江理工大学生命科学学院, 杭州 310018; 2. 第二军医大学东方肝胆外科医院, 上海 200438
Author(s):
 L Saiqun JIN Huajun QIAN Qijun
 1. School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China; 2. Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, China
关键词:
 miR29b 甲基化转移酶 DNMT3A DNMT3B
分类号:
Q789
文献标志码:
A
摘要:
     DNA甲基化异常在肿瘤发生中起着极为重要的作用,而基因组DNA的甲基化主要通过DNA甲基化转移酶(DNA methyhransferases,DNMTs)来完成。microRNA和甲基化转移酶的表达具有一定的联系。构建携带有miR29b的腺病毒载体并包装病毒,鉴定正确后,将病毒感染肝癌细胞PLC,用qRTPCR的方法检测miR29b在PLC细胞中对甲基化转移酶表达的调控作用。结果表明,成功构建携带有miR29b的腺病毒,qRTPCR方法检测表明miR29b能有效地调控甲基化转移酶的表达。

参考文献/References:

 [1] Esteller M. CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future[J]. Oncogene, 2002, 21(35): 54275440.
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[7] Rhee I, Bachman K E, Park B H, et al. DNMT1 and DNMT3b cooperate to silence genes in human cancer cells[J]. Nature, 2002, 416: 552556.
[8] Fang J Y, Lu R, Mikovits J A, et al. Regulation of hMSH2 and hMLH1 expression in the human colon cancer cell line SW1116 by DNA methyltransferase 1[J]. Cancer Lett, 2006, 233(1): 124130.
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备注/Memo

备注/Memo:
 收稿日期: 2010-01-08
基金项目: 国家自然科学基金(30730104)
作者简介: 吕赛群(1985-),女,浙江嵊州人,硕士研究生,从事肿瘤干细胞以及靶向基因—病毒治疗研究。
通讯作者: 钱其军,电子邮箱:qianqj@sinogene.com
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