|本期目录/Table of Contents|

[1]金鲁宁,李林芳,钱其军. 携带Herceptin与MICA胞外结构域融合基因的重组腺病毒的构建及鉴定[J].浙江理工大学学报,2011,28(01):106-110.
 JIN Lu ning,LI Lin fang,QIAN Qi jun. Construction and Identification of a Recombinant Adenovirus Vector Carrying a Fusion Gene Composed of Heceptin and the ECD of MICA[J].Journal of Zhejiang Sci-Tech University,2011,28(01):106-110.
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 携带Herceptin与MICA胞外结构域融合基因的重组腺病毒的构建及鉴定()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
106-110
栏目:
生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Construction and Identification of a Recombinant Adenovirus Vector Carrying a Fusion Gene Composed of Heceptin and the ECD of MICA
文章编号:
16733851 (2011) 01010605
作者:
 金鲁宁1 李林芳2 钱其军12
 1. 浙江理工大学生命科学学院, 杭州 310018; 2. 第二军医大学东方肝胆外科医院病毒基因治疗实验室, 上海 200438
Author(s):
 JIN Luning1 LI Linfang2 QIAN Qijun12
 1. School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China; 
2. Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, China
关键词:
 Herceptin MICA 腺病毒
分类号:
Q786
文献标志码:
A
摘要:
     通过构建能表达抗体Herceptin与MICA胞外结构域的融合蛋白的重组腺病毒,为进一步联合NK细胞的治疗研究奠定基础。采用OverlapPCR的方法获得Herceptin与MICA胞外结构域的融合基因,并将融合基因连接到载体pDC339上。接着再利用酶切及PCR方法鉴定载体及重组病毒基因的正确性,并通过ELISA和Western Blot检测融合蛋白的表达情况,最后经间接免疫荧光分析(IFA)检测融合蛋白的结合特性。构建的载体酶切后经电泳鉴定证实构建成功,重组病毒DNA的PCR显示病毒携带了Herceptin的轻链基因、重链基因及MICA胞外结构域的基因;ELISA和Western Blot证实重组腺病毒成功地表达出了目的融合蛋白;IFA实验表明融合蛋白能与SKOV3细胞表面的Her2受体特异性地结合。最后成功地构建了能表达具有正常结合特性的抗体Herceptin与MICA胞外结构域的融合蛋白的重组腺病毒,为之后与NK细胞的联合治疗研究奠定基础。

参考文献/References:

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[7] Zhang Bin, Wei Haiming, Zheng Xiaodong, et al. The inhibitory effects of synthetic short peptides, mimicking MICA and targeting at NKG2D receptors, on function of NK cells[J]. Peptides, 2005, 26(3): 405412.

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备注/Memo

备注/Memo:
 收稿日期: 2010-01-08
基金项目: 国家新药创制重大专项课题(2009ZX09103687)
作者简介: 金鲁宁(1986-),男,浙江温州人,硕士研究生,主要从事肿瘤基因治疗方面的研究。
通讯作者: 钱其军,电子邮箱:qianqj@163.com
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