|本期目录/Table of Contents|

[1]李恩书,彭新荣,钱其军. 无血清条件下LIF对小鼠胚胎干细胞增殖及多能性的影响[J].浙江理工大学学报,2011,28(01):96-100.
 LI En shu,PENG Xin rong,QIAN Qi jun. Effect of LIF on the Mouse Embryo Stem Cells under SerumFree Condition[J].Journal of Zhejiang Sci-Tech University,2011,28(01):96-100.
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 无血清条件下LIF对小鼠胚胎干细胞增殖及多能性的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
96-100
栏目:
(自科)生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Effect of LIF on the Mouse Embryo Stem Cells under SerumFree Condition
文章编号:
1673\|3851 (2011) 01\|0096\|05
作者:
 李恩书1 彭新荣2 钱其军12
 1. 浙江理工大学新元医学与生物技术研究所, 杭州 310018; 2. 第二军医大学东方肝胆外科医院病毒治疗实验室, 上海 200438
Author(s):
 LI Enshu1 PENG Xinrong2 QIAN Qijun12
 1. Xinyuan Institute of Medicine and Biotechnology, Zhejiang SciTech University, Hangzhou 310018, China; 2. Eastern Hepatobiliary Hospital VirusGene Therapy Laboratories, The Second Military Medical University, Shanghai 200438, China
关键词:
 李恩书1 彭新荣2 钱其军12
分类号:
Q813.11
文献标志码:
A
摘要:
    在无血清条件下培养KES小鼠胚胎干细胞,研究LIF在无血清条件下对小鼠胚胎干细胞增殖及多能性维持的影响。联合使用小分子物质SU5402、PD184352和CHIR99021或单独使用LIF培养小鼠胚胎干细胞,检测无血清条件下LIF对小鼠胚胎干细胞自我更新能力和全能性的影响。结合使用极限稀释法培养KES细胞单克隆,统计不同培养体系下单克隆的形成率。结果显示:无血清条件下,单独使用LIF,不能维持小鼠胚胎干细胞的自我更新能力和全能性;使用含有3种小分子药物 SU5402, PD184352和CHIR99021(3i)的无血清培养液可以维持小鼠胚胎干细胞的自我更新,传代60代以上仍可保持全能性;添加LIF的3i培养系统显著提高了小鼠胚胎干细胞的单克隆形成率。表明在无血清条件下,LIF不能单独维持小鼠胚胎干细胞的多能性。3i培养体系可以维持胚胎干细胞的全能性而不需要LIF;3i条件下,LIF可以显著促进胚胎干细胞体外增殖和单克隆形成。

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备注/Memo

备注/Memo:
 收稿日期: 2010-01-08
基金项目: 国家自然科学基金海外杰出青年项目(30728028)
作者简介: 李恩书(1985-),女,浙江杭州人,硕士研究生,主要从事胚胎干细胞及iPS研究。
通讯作者: 钱其军,电子邮箱:qianqj@163.com
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