|本期目录/Table of Contents|

[1]夏玉龙,马步云,肖睿娟,等. 携带Kallistatin的溶瘤腺病毒载体构建及其对肝癌细胞的体外杀伤效应[J].浙江理工大学学报,2012,29(04):594-598.
　XIA Yu long,MA Bu yun,XIAO Rui juan,et al. Construction of Oncolytic Adenovirus Vector Carrying Kallistatin andIts KillingEffect to Liver Cancer Cells in Vitro[J].Journal of Zhejiang Sci-Tech University,2012,29(04):594-598.
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 携带Kallistatin的溶瘤腺病毒载体构建及其对肝癌细胞的体外杀伤效应()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年04期
页码:
594-598
栏目:
出版日期:
2012-08-28

文章信息/Info

Title:
 Construction of Oncolytic Adenovirus Vector Carrying Kallistatin andIts KillingEffect to Liver Cancer Cells in Vitro
文章编号:
16733851 (2012) 04059405
作者:
 夏玉龙;  马步云;  肖睿娟;  雷文;  杨远勤;  王毅刚
 浙江理工大学生命科学学院新元医学与生物技术研究所, 杭州 310018
Author(s):
 XIA Yulong;  MA Buyun;  XIAO Ruijuan;  LEI Wen;  YANG Yuanqin;  WANG Yigang
 Xinyuan Institute of Medicine and Biochnology, School of Life Sciences, ZhejiangSciTech University, Hangzhou 310018, China
关键词:
 ; ZD55KAL;  MTT;  结晶紫染色;  靶向基因-病毒治疗
分类号:
Q789
文献标志码:
A
摘要:
    采用靶向基因-病毒治疗策略，通过同源重组的方式构建了携带Kallistatin（KAL）的重组溶瘤腺病毒ZD55KAL，并研究其对肝癌细胞的杀伤作用。通过PCR方法鉴定病毒构建正确；MTT法检测病毒对肝癌细胞的杀伤能力和对正常细胞的安全性；结晶紫染色观察细胞凋亡现象。结果显示：经目的病毒ZD55KAL感染后肝癌细胞出现明显的病变和生长抑制，而正常细胞未出现病变现象，表明目的病毒具有较高的安全性和对肿瘤细胞的特异性杀伤能力。此外ZD55KAL感染肝癌细胞后引起凋亡，所携带的治疗基因KAL能通过促进肿瘤的凋亡达到抑制肿瘤细胞生长的效果。

参考文献/References:

 ［1］ Zhang Z L, Liu X Y, Zou W G, et al. An armed oncolytic adenovirus system, ZD55gene, demonstrating potent antitumoral efficacy［J］. Cell Res, 2003, 13(6): 481489.
［2］ Shen B, Gao L, Hsu Y T, et al. Kallistatin attenuates endothelial apoptosis through inhibition of oxidative stress and activation of AkteNOS signaling［J］. Am J Physiol Heart Circ Physiol, 2010, 299(5): 14191427.
［3］ Chao J. Novel roles of kallistatin, a specific tissue kallikrein inhibitor, in vascular remodeling［J］. Biol Chem, 2001, 382(1): 1521.
［4］ Miao R Q, Agata J, Chao L, et al. Kallistatin is a new inhibitor of angiogenesis and tumor growth［J］. Blood, 2002, 100(9): 32453252.
［5］ Jeschke U, Schiessl B, Mylonas I, et al. Expression of the proliferation marker Ki67 and of p53 tumor protein in trophoblastic tissue of preeclamptic, HELLP, and intrauterine growthrestricted pregnancies［J］. Int J Gynecol Pathol, 2006, 25(4): 354360.
［6］ Diamandis E P, Yousef G M. Human tissue kallikreins: a family of new cancer biomarkers［J］. Clin Chem, 2002, 48(8): 11981205.
［7］ Su Z Z, Goldstein N I, Jiang H, et al. PEG3, a nontransforming cancer progression gene, is a positive regulator of cancer aggressiveness and angiogenesis［J］. Proc Natl Acad Sci USA, 1999, 96(26): 1511515120.
［8］ MethenyBarlow, Linda J, Li L Y. Vascular endothelial growth inhibitor (VEGI), an endogenous negative regulator of angiogenesis［J］. Semin Ophthalmol, 2006, 21(1): 4958.
［9］ Chow T F, Earle T, ElSaid H, et al. Kallikreins as microRNA targets: an in silico and experimentalbased analysis［J］. Biol Chem, 2008, 389(6): 731738.〖ZK)〗
［10］ Talieri M L. The 2nd International Symposium on Kallikreins and KallikreinRelated Peptidases (ISK 2007) and the Commemorative Gold Medal of the E.K. FreyE. Werle Foundation of the Henning L. Voigt Family［J］. Biol Chem, 2008, 389(6): 621622.
［11］ Rabien A. Human kallikreins as tumor markers: Validation of potential marker of prostate cancer in serum and tissue［J］. Urologe A, 2007, 46(9): 10701071.

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备注/Memo

备注/Memo:
 收稿日期： 2011-11-16
基金项目： 国家自然科学基金项目(30800093)；浙江省大学生科技创新计划课题（2010R406036）；浙江理工大学科研启动基金项目（1016845-Y,1016834-Y）
作者简介： 夏玉龙（1986-），男，安徽合肥人，硕士研究生，主要从事肿瘤的靶向基因-病毒治疗的研究。
通讯作者： 王毅刚，电子邮箱：wangyigang43@163.com
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