|本期目录/Table of Contents|

[1]梁晨,单娟娟,沈俊杰,等. 肝癌细胞系Huh7中肿瘤干细胞分离及鉴定[J].浙江理工大学学报,2011,28(04):601-605.
　LIANG Chen,SHAN Juan juan,SHENG Jun jie,et al. Isolation and Characterization of Cancer Stem Cells from a Human Hepatocellular Carcinoma Cell Lines Huh7[J].Journal of Zhejiang Sci-Tech University,2011,28(04):601-605.
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 肝癌细胞系Huh7中肿瘤干细胞分离及鉴定()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年04期
页码:
601-605
栏目:
(自科)生物与生命科学
出版日期:
2011-08-31

文章信息/Info

Title:
 Isolation and Characterization of Cancer Stem Cells from a Human Hepatocellular Carcinoma Cell Lines Huh7
文章编号:
16733851 (2011) 04060204
作者:
 梁晨;  单娟娟;  沈俊杰;  刘立;  钱程
 浙江理工大学生命科学学院, 杭州 310018
Author(s):
 LIANG Chen;  SHAN Juanjuan;  SHENG Junjie;  LIU Li;  QIAN Cheng
 Shool of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; 肿瘤干细胞;  肝癌（干）细胞球;  生长因子;  EpCAM
分类号:
Q786
文献标志码:
A
摘要:
    利用肿瘤干细胞具有自我更新能力的特点，首次采用悬浮培养技术从Huh7细胞系中得到肝癌（干）细胞球，并通过RT\|PCR、流式细胞术和免疫荧光等方法检测它们的干细胞标记和分化标记。结果显示Huh7（干）细胞球的干性相关基因表达上调；分化相关基因表达下调；流式细胞仪检测结果表明88.82%的Huh7（干）细胞球表达EpCAM基因，与正常Huh7细胞相比表达上调。表明该方法具有富集肝癌干细胞的效果，为以后肝癌干细胞建立更高效的分离方法提供了一种新的技术方法。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2010-11-12
基金项目: 国家自然科学基金面上项目（30872984）；重庆市自然科学基金计划项目(CSTC 09BB5024)
作者简介： 梁晨（1986-），女，山东济南人，硕士研究生，主要从事肿瘤基因病毒治疗方面的研究。
通讯作者： 刘立，E\|mail：liuli7001@yahoo.com.cn
更新日期/Last Update: