|本期目录/Table of Contents|

[1]仇庆,谢国良,谌贺宽子,等. 组蛋白去乙酰化酶抑制剂SAHA联合ZD55TRAIL溶瘤腺病毒对肝癌细胞杀伤作用的研究[J].浙江理工大学学报,2012,29(03):418-422.
 QIU Qing,XIE Guo liang,CHEN He kuan zi,et al. Study on Targeting Oncolytic Adenovirus that ZD55TRAIL Linked Suberoylanilide hydroxamic acid (SAHA) for Liver Tumor Cells Therapy[J].Journal of Zhejiang Sci-Tech University,2012,29(03):418-422.
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 组蛋白去乙酰化酶抑制剂SAHA联合ZD55TRAIL溶瘤腺病毒对肝癌细胞杀伤作用的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年03期
页码:
418-422
栏目:
生物与生命科学
出版日期:
2012-06-28

文章信息/Info

Title:
 Study on Targeting Oncolytic Adenovirus that ZD55TRAIL Linked Suberoylanilide hydroxamic acid (SAHA) for Liver Tumor Cells Therapy
文章编号:
16733851 (2012) 03041805
作者:
 仇庆 谢国良 谌贺宽子 李敏 周秀梅
 浙江理工大学生命科学院, 杭州 310018
Author(s):
 QIU Qing XIE Guoliang CHEN Hekuan zi LI Min ZHOU Xiumei
 Xinyuan Institute of Medicine and Biotechnology, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 肿瘤/基因治疗 SAHA ZD55TRAIL 肝癌细胞株 凋亡
分类号:
Q789
文献标志码:
A
摘要:
    研究组蛋白去乙酰化抑制剂SAHA(suberoylanilide hydroxamic acid)联合ZD55TRAIL对人肝癌细胞株的体外杀伤效果,实验采用MTT法检测SAHA、ZD55TRAIL以及二者联合对肝癌细胞株Huh7、Bel7404、Hep3B以及人正常肝细胞株QSG7701增殖的抑制作用;利用Hoechst33342染色对联合处理的细胞进行凋亡形态学观察;通过结晶紫实验进一步检测联合用药对肿瘤细胞的杀伤情况;通过Western blot实验在蛋白水平上检测一些凋亡相关蛋白表达情况的变化。实验结果表明:SAHA联合ZD55TRAIL处理3 d后对肝癌细胞株Huh7的增殖抑制率达到50% ,对Hep3B、Bel7404也达到近40%的杀伤效率,并且联合治疗后对人正常细胞株QSG7701未见明显的毒副作用。同时结晶紫实验也证明联合治疗对肿瘤细胞的杀伤力强于用SAHA或ZD55TRAIL单独使用。Western blot实验证明了药物和病毒的联合作用使促凋亡蛋白Bcl2,BclxL的表达量明显减少,caspase8和caspase9蛋白也有相应剪切现象的发生。流式细胞仪同样检测出SAHA和ZD55TRAIL联合作用对肝癌细胞有很好的杀伤作用。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2011-06-22
基金项目: 国家自然科学基金(81071850);浙江省大学生创新创业孵化项目(2010R405069);浙江理工大学科研启动基金(1016834Y)
作者简介: 仇庆(1987-),女,湖南汨罗人,硕士研究生,主要从事肿瘤的靶向基因治疗研究。
通讯作者: 周秀梅,Email:zhouxiumei824@163.com
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