|本期目录/Table of Contents|

[1]高珍,卜远虹,金邦泽,等. 家蚕新基因Bmbzj的表达、抗体制备及亚细胞定位[J].浙江理工大学学报,2012,29(03):414-417.
 GAO Zhen,BU Yuan hong,JIN Bang ze,et al. Cloning, Expression and Subcellular Localization of a Novel Gene Bmbzj from Silkworm Pupae (Bombyx mori[J].Journal of Zhejiang Sci-Tech University,2012,29(03):414-417.
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 家蚕新基因Bmbzj的表达、抗体制备及亚细胞定位()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年03期
页码:
414-417
栏目:
生物与生命科学
出版日期:
2012-06-28

文章信息/Info

Title:
 Cloning, Expression and Subcellular Localization of a Novel Gene Bmbzj from Silkworm Pupae (Bombyx mori
文章编号:
16733851 (2012) 03041404
作者:
 高珍1 卜远虹1 金邦泽1 于威1 刘悦2 陈健1 张耀洲1
 1. 浙江理工大学生物化学研究所 杭州, 310018; 2. 浙江经贸职业技术学院应用工程系 杭州, 310018
Author(s):
 GAO Zhen1 BU Yuanhong 1 JIN Bangze 1 YU Wei 1 LIU Yue2CHEN Jian1 ZHANG Yaozhou1
 1. Institute of Biochemistry, Zhejiang SciTech University, Hangzhou 310018, China;2. School of Applied Technology, Zhejiang Economic & Trade Polytechnic, Hangzhou 310018, China
关键词:
 家蚕 Bmbzj基因 原核表达 纯化 亚细胞定位
分类号:
Q74
文献标志码:
A
摘要:
     在自建的家蚕蛹cDNA文库中发现了一条新基因序列,GenBank登录号为DY231426。该基因是一个同源性很低的基因,未发现保守结构域,将其命名为Bmbzj。Bmbzj基因全长661 bp,由 99 bp的 5′端非翻译区序列(5′UTR)、486 bp的开放读码框(ORF)和76 bp的 3′端非翻译区序列(3′UTR)组成。生物信息学分析结果表明蛋白Bmbzj可能存在跨膜区和信号肽。将该基因插入到载体pGEX4T3中,构建了该基因编码的重组质粒pGEX4T3Bmbzj,转化大肠杆菌Rosetta后,IPTG诱导表达了该融合蛋白,并利用亲和层析的方法纯化该重组蛋白,免疫新西兰兔制备多克隆抗体。亚细胞定位实验结果表明该蛋白只存在于细胞质中。

参考文献/References:

 [1] Xia Q Y, Zhou Z Y, Lu C, et al. A draft sequence for the genome of the domesticated silkworm [J]. Science, 2004, 306(10): 19371940.
[2] Adams M D, Kelley J M, Gocayne J D, et al. Complementary DNA sequencing: expressed sequence tags and human genome project[J]. Science 1991, 252: 16511656.
[3] 冯浩咏, 苗向阳, 罗绪刚, 等. 生物信息学在发现新基因方面的应用[J]. 生物技术通报, 2007(5): 15.
[4] 唐雪芳, 贺修胜. 生物信息学策略鉴定新基因[J]. 现代生物医学进展, 2008, 8(7): 13581360.
[5] Chen J, Chen J, Gai Q, et al. Molecular characterization and immunhistochemical localization of a novel troponin C during silkworm development [J]. Cell Tissue Res, 2008, 331(3): 725738.
[6] Liu S, Huang C Q, Li D W, et al. Molecular cloning and expression analysis of a new gene for short chain dehydrogenase/reductase 9[J]. Acta Biochimica Polonica, 2007, 54(1): 213218.
[7] Green P, Lipman D, Hillier L, et al. Ancient conserved regions in new gene sequences and the protein databases[J]. Science, 1993, 259(5102): 17111716.

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备注/Memo

备注/Memo:
 收稿日期: 2011-05-23
基金项目: 国家高技术研究发展计划(863计划,2011AA100603);浙江省自然科学基金项目(Y3090304,Y3110354)
作者简介: 高珍(1987-),女,安徽安庆人,硕士研究生,研究方向为生物化学与分子生物学。
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