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[1]张瑾莉,杨希,朱丽娅,等.栝楼籽蛋白酶解物对氧化损伤HepG2细胞的保护作用[J].浙江理工大学学报,2022,47-48(自科六):908-914.
　ZHANG Jinli,YANG Xi,ZHU Liya,et al.Protective effect of protease hydrolysates of trichosanthes seed on oxidatively damaged HepG2 cells[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科六):908-914.
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栝楼籽蛋白酶解物对氧化损伤HepG2细胞的保护作用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第47-48卷
期数:
2022年自科第六期
页码:
908-914
栏目:
出版日期:
2022-11-10

文章信息/Info

Title:
Protective effect of protease hydrolysates of trichosanthes seed on oxidatively damaged HepG2 cells
文章编号:
1673-3851 (2022) 11-0908-07
作者:
张瑾莉; 杨希; 朱丽娅; 袁翔宇
1.安徽粮食工程职业学院，合肥 230011；2.合肥工业大学食品与生物工程学院，合肥 230601
Author(s):
ZHANG Jinli; YANG Xi; ZHU Liya; YUAN Xiangyu
1.Department of Food Biology, Anhui Vocational College of Grain Engineering,  Hefei 230011, China; 2.School of Food and Biological Engineering, Hefei  University of Technology, Hefei 230601, China
关键词:
栝楼籽蛋白酶解物; H2O2; 氧化损伤; HepG2; 抗氧化作用
分类号:
TS195-644
文献标志码:
A
摘要:
为研究栝楼籽蛋白酶解物(Protease hydrolysates of trichosanthes seed，PHTS)对氧化损伤的人肝癌细胞株HepG2的抗氧化保护作用，构建H2O2诱导的HepG2氧化损伤细胞模型，检测细胞存活率、细胞总谷胱甘肽（Glutathione，GSH）及细胞分泌NO情况；并分析过氧化氢酶（Catalase，CAT）和超氧化物歧化酶(Superoxide dismutase，SOD)的表达情况。分别设置空白对照组、H2O2损伤组、PHTS实验组和GSH阳性对照组。结果显示：与空白对照组相比，H2O2损伤组的细胞存活率、细胞总GSH水平、NO分泌量、抗氧化酶CAT和SOD基因表达与蛋白表达水平均显著降低；在PHTS实验组，细胞存活率、总GSH水平、NO分泌量、CAT和SOD的基因表达与蛋白表达水平均明显增加(p ＜0.05)，表明栝蒌籽蛋白酶解物可有效增强HepG2细胞的抗氧化保护作用。因此，栝楼籽蛋白酶解物具有细胞水平的抗氧化作用，有望应用于氧化应激所致肝脏疾病的辅助治疗。

参考文献/References:

［ 1 ］ Li S, Tan H Y, Wang N, et al. The role of oxidative stress and antioxidants in liver diseases ［ J ］ . International Journal of Molecular Sciences, 2015, 16(11): 26087-26124.

［2］Yang S, Liao W B. Hydroxysafflor yellow A attenuates oxidative stress injury-induced apoptosis in the nucleus pulposus cell line and regulates extracellular matrix balance via CA Ⅻ［J］. Experimental and Therapeutic Medicine, 2022, 23(2): 182.

［3］Milena V, Duan M, Marina M, et al. The effects of Betaine on oxidative stress, inflammation, apoptosis, and autophagy in methionine-choline deficient diet-induced fatty liver disease in mice［J］. Pathophysiology, 2018, 25(3): 233-234.

［4］Karim N, Shishir M R I, Rashwan A K, et al. Suppression of palmitic acid-induced hepatic oxidative injury by neohesperidin-loaded pectin-chitosan decorated nanoliposomes［J］. International Journal of Biological Macromolecules, 2021, 183: 908-917.

［5］Zhao H, Song L. TKP, a serine protease from Trichosanthes kirilowii, inhibits cell proliferation by blocking aerobic glycolysis in hepatocellular carcinoma cells［J］. Nutrition and Cancer, 2021, 74(1): 11-13.

［6］熊利芝, 吴玉先, 曹卫君, 等. 栝楼籽蛋白分离纯化及抗氧化性的研究［J］. 应用化工, 2016, 45(1): 7-10.

［7］马萍, 程天赋, 郭增旺, 等.紫花芸豆肽修复H 2O 2对HepG2细胞的氧化应激损伤［J］. 食品科学, 2020, 41(1): 175-182.

［8］Sun J, Zhou C Y, Cao J X, et al. Purification and characterization of novel antioxidative peptides from duck liver protein hydrolysate as well as their cytoprotection against oxidative stress in HepG2 Cells［J］. Frontiers in Nutrition, 2022, 9: 848289.

［9］Hu Y M, Lu S Z, Li Y S, et al. Protective effect of antioxidant peptides from grass carp scale gelatin on the H 2O 2-mediated oxidative injured HepG2 cells［J］. Food Chemistry, 2021, 373: 131539.

［10］Mattia A, Desire B, Maria R C, et al. Imbalance in the antioxidant defence system and pro-genotoxic status induced by high glucose concentrations: in vitro testing in human liver cells［J］. Toxicology in Vitro, 2020, 69: 105001.

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备注/Memo

备注/Memo:
收稿日期： 2022-05-04
网络出版日期：2022-07-08
基金项目： 安徽省自然科学研究项目(KJ2019A1267)；安徽省教育厅高校优秀拔尖人才培育资助项目(gxgnfx2020179)；安徽省职业与成人教育学会教育科研规划课题(azcg141)
作者简介： 张瑾莉(1988-)，女，安徽安庆人，讲师，硕士，主要从事生物化学方面的研究。
通信作者： 张瑾莉，E-mail:422416354@qq.com
更新日期/Last Update: 2022-11-07