|本期目录/Table of Contents|

[1]钱央央,黄路瑶,范浩宇,等.铁皮石斛组培苗和栽培苗的lncRNA检测及其差异表达[J].浙江理工大学学报,2022,47-48(自科四):615-622.
 QIAN Yangyang,HUANG Luyao,FAN Haoyu,et al.Detection and differential expression of lncRNA in tissue cultured seedings and cultivated seedings of Dendrobium officinale[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科四):615-622.
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铁皮石斛组培苗和栽培苗的lncRNA检测及其差异表达()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第47-48卷
期数:
2022年自科第四期
页码:
615-622
栏目:
出版日期:
2022-09-30

文章信息/Info

Title:
Detection and differential expression of lncRNA in tissue cultured seedings and cultivated seedings of Dendrobium officinale
文章编号:
1673-3851 (2022) 07-0615-08
作者:
钱央央黄路瑶范浩宇徐涛
浙江理工大学生命科学与医药学院,杭州 310018
Author(s):
QIAN Yangyang HUANG Luyao FAN Haoyu XU Tao
College of Life Sciences and Medicine, Zhejiang Sci-Tec University, Hangzhou 310018, China
关键词:
铁皮石斛菌根高通量测序lncRNA差异性表达
分类号:
Q94-3
文献标志码:
A
摘要:
为了研究铁皮石斛菌根共生系统的Long noncoding RNA(lncRNA)调控机制,采用高通量测序方法对铁皮石斛栽培苗和组培苗lncRNA进行检测,并分析显著差异转录本。结果显示:2组4个样品共测出26543个lncRNA转录本,在实验组中显著上调的有186组,显著下调的有183组。GO富集分析共获得1275组数据,具有显著性差异的基因数最多的是细胞核;KEGG富集分析共获得20条通路,其中富集程度最显著的是苯丙素生物合成途径。选取参考基因已知的转录本,得到10组显著上调的lncRNA,10组显著下调的lncRNA,共涉及5条富集通路,分别在淀粉和蔗糖代谢途径和苯丙素生物合成途径中上调,在植物激素信号转导和内质网中的蛋白质加工途径中下调,剪接体途径中则同时存在表达上调和下调。以上结果可为进一步研究铁皮石斛菌根共生机制提供参考,对相关功能进行预测。

参考文献/References:

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[8] Qin T, Zhao H Y, Cui P, et al. A nucleuslocalized long noncoding RNA enhances drought and salt stress tolerance[J]. Plant Physiology, 2017, 175(3): 1321-1336.

[9] 高正华, 杨兵勋, 陈立钻. 铁皮石斛的研究进展[J]. 中国现代应用药学, 2008, 25(8): 692-695.

[10] Feng S G, He R F, Yang S, et al. Start codon targeted (SCoT) and target region amplification polymorphism (TRAP) for evaluating the genetic relationship of Dendrobium species[J]. Gene, 2015, 567(2): 182-188.

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备注/Memo

备注/Memo:
收稿日期:2021-10-20
网络出版日期:2022-01-05
基金项目:浙江省重点研发计划项目(2015C02030)
作者简介:钱央央(1995-),女,杭州人,硕士研究生,主要从事天然药用植物资源开发方面的研究
通信作者:徐涛,E-mail:xutao1998cn@163.com
更新日期/Last Update: 2022-09-06