|本期目录/Table of Contents|

[1]黄路瑶,郁吉锋,钱央央,等.铁皮石斛组培苗和栽培苗的circRNA检测及分析[J].浙江理工大学学报,2020,43-44(自科六):840-845.
 HUANG Luyao,YU Jifeng,QIAN Yangyang,et al.Detection and analysis of circRNA in tissue cultured seedlinog and cultivated seedling of Dendrobium officinale[J].Journal of Zhejiang Sci-Tech University,2020,43-44(自科六):840-845.
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铁皮石斛组培苗和栽培苗的circRNA检测及分析()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第43-44卷
期数:
2020年自科六期
页码:
840-845
栏目:
出版日期:
2020-11-27

文章信息/Info

Title:
Detection and analysis of circRNA in tissue cultured seedlinog and cultivated seedling of Dendrobium officinale
文章编号:
1673-3851 (2020) 11-0840-06
作者:
黄路瑶郁吉锋钱央央徐涛
浙江理工大学生命科学与医药学院,杭州 310018
Author(s):
HUANG LuyaoYU JifengQIAN YangyangXU Tao
College of Life Sciences and Medicine, Zhejiang Sci-tech University, HangZhou 310018, China
关键词:
铁皮石斛组培苗栽培苗circRNA差异性表达
分类号:
Q94-3
文献标志码:
A
摘要:
为了分析铁皮石斛组培苗与有菌根的栽培苗的circular RNA (circRNA)变化,采用高通量测序方法对铁皮石斛组培苗和栽培苗进行circRNA检测,并分析它们之间的差异。结果显示:两组4个样品共测出4115组不同的circRNA,其中有显著性差异的有27种,在实验组中17种为显著上调,10种为显著下调;GO富集分析一共检测出99组数据,其中显著性基因最多是核基因;KEGG富集分析表明,circRNA共有富集在7条KEGG通路上,其中丁酸甲酯代谢途径(Butanoate metabolism)和错配修复途径(Mismatch repair)具有显著性差异。以上结果可为进一步研究铁皮石斛形成菌根前后circRNA的变化提供参考。

参考文献/References:

[1] Dong R, Ma X K, Chen L L, et al. Increased complexity of circRNA expression during species evolution[J]. RNA Biology, 2017, 14(8):1064-1074.
[2] Liu Y Q, Zhang X, Liu M, et al. Direct detection of circRNA in real samples using reverse transcriptionrolling circle amplification[J]. Analytica Chimica Acta, 2020, 1101: 169-175.
[3] George A K, Master K, Majumder A, et al. Circular RNAs constitute an inherent gene regulatory axis in the mammalian eye and brain[J]. Canadian Journal of Physiology And Pharmacology, 2019, 97(6):463-472.
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[5] Guo R, Chen D F, Chen H Z, et al. Systematic investigation of circular RNAs in Ascosphaera apis, a fungal pathogen of honeybee larvae[J]. Gene, 2018, 678: 17-22.
[6] He L B, Zhang A D, Xiong L, et al. Deep circular RNA sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection[J]. International Journal of Molecular Sciences, 2017, 18(9):1977-1993.
[7] Zhang P J, Meng X W, Chen H J, el. PlantCircNet: A database for plant circRNAmiRNAmRNA regulatory networks[J]. DatabaseThe Journal of Biological Databases and Curation, 2017, 11: 1-8.
[8] Huo L, Zhang P, Li C X, et al. Genomewide identification of circRNAs in pathogenic basidiomycetous yeast Cryptococcus neoformans suggests conserved circRNA host genes over kingdoms[J]. Genes, 2018, 9(3):118-131.
[9] 刘致新, 孙文洲, 李佳欣, 等. circRNA的生物学功能及其在卵巢癌中的研究进展[J]. 癌症进展, 2020, 18(4):347-350.
[10] 张晓云, 孔鹏, 窦永青, 等. circRNA肿瘤相关生物学功能的研究进展[J]. 生命的化学, 2020, 40(4): 607-613.

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备注/Memo

备注/Memo:
收稿日期:2020-06-28
网络出版日期:2020-09-03
基金项目:浙江省重点研发计划项目(2015C02030)
作者简介:黄路瑶(1995-),女,江西宜春人,硕士研究生,主要从事天然药用植物资源开发方面的研究
通信作者:徐涛,E-mail:xutao1998cn@163.com
更新日期/Last Update: 2020-11-05