|本期目录/Table of Contents|

[1]赵景川,严巧灵,钱其军. 靶向肝癌细胞的嵌合AAV建库及筛选[J].浙江理工大学学报,2012,29(06):874-878.
 ZHAO Jing chuan,YAN Qiao ling,QIAN Qi jun. The Libraries Building and Screening of Chimeric AAVTargeting Liver Cancer Cells[J].Journal of Zhejiang Sci-Tech University,2012,29(06):874-878.
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 靶向肝癌细胞的嵌合AAV建库及筛选()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年06期
页码:
874-878
栏目:
生物与生命科学
出版日期:
2012-12-28

文章信息/Info

Title:
 The Libraries Building and Screening of Chimeric AAVTargeting Liver Cancer Cells
文章编号:
16733851 (2012) 06087405
作者:
 赵景川1 严巧灵2 钱其军12
 1. 浙江理工大学生命科学学院 新元医学与生物技术研究所, 杭州 310018;2. 第二军医大学东方肝胆医院 病毒基因治疗实验室, 上海 200438
Author(s):
 ZHAO Jingchuan1 YAN Qiaoling2 QIAN Qijun12
 1. Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang SciTechUniversity, Hangzhou 310018, China; 2. Laboratory of Virus and Gene Therapy, EasternHepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, China
关键词:
 Shuffling 嵌合AAV 靶向 肝癌细胞
分类号:
Q782
文献标志码:
A
摘要:
    采用Shuffling的方法,将AAV19 Cap基因序列进行改组,筛选出高效靶向肝癌细胞的嵌合AAV载体。以pAAVMCS载体为骨架,构建出含AAV2 ITR和Rep2序列的pAdB载体,在此基础上,构建含有嵌合Cap序列的pARC载体库,用于转染293细胞,并加入5型腺病毒(Ad5),包装出嵌合AAV病毒。然后将包装出的嵌合AAV病毒与Ad5共感染BEL7402肝癌细胞,经过三轮筛选后,初步筛选出可以靶向肝癌细胞的嵌合AAV。最后将筛选出的AAV Rep2嵌合Cap基因的融合片段连接到pMD18T载体上,构建出pARCT载体,与AAVEGFP质粒共转染293细胞,并加入Ad5,包装出病毒基因组含EGFP基因、病毒外壳为嵌合Cap的AAV病毒(cAAVEGFP),并用其感染BEL7402细胞,观察绿色荧光的表达。最后观察到绿色荧光表达,证明筛选出了靶向BEL7402肝癌细胞的嵌合AAV。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2012-02-15
基金项目: 赵景川(1983-),男,山东临沂人,硕士研究生,主要从事肿瘤的靶向基因病毒治疗方面的研究。
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