|本期目录/Table of Contents|

[1]赵清霞,王春红,钱其军. 昆虫杆状病毒表达egfp载体构建及鉴定[J].浙江理工大学学报,2011,28(01):122-125.
 ZHAO Qing xia,WANG Chun hong,QIAN Qi jun. Construction and Identification of Baculovirus Expression Vector with Enhanced Green Fluorescent Protein[J].Journal of Zhejiang Sci-Tech University,2011,28(01):122-125.
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 昆虫杆状病毒表达egfp载体构建及鉴定()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
122-125
栏目:
生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Construction and Identification of Baculovirus Expression Vector with Enhanced Green Fluorescent Protein
文章编号:
1673\|3851 (2011) 01\|0122\|04
作者:
 赵清霞1 王春红2 钱其军12
 1. 浙江理工大学新元医学与生物技术研究所, 杭州 310018; 2. 上海第二军医大学东方肝胆外科医院基因病毒实验室, 上海 200438
Author(s):
 ZHAO Qingxia1 WANG Chunhong2 QIAN Qijun12
 1. Xinyuan Institute of Medicine and Biotechnology, Zhejiang SciTech University, Hangzhou 310018, China; 2. Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgical Hospital, The Second Militaty Medical University, Shanghai 200438, China
关键词:
 egfp 杆状病毒 Sf9细胞
分类号:
Q789
文献标志码:
A
摘要:
    用EcoR I和Sal I将目的基因片段6His11ReGFP从载体pDC3166His11ReGFP上酶切下来,并将该目的基因片段与供体载体pFastBac1连接构建供体质粒pFastBac16His11ReGFP;将其转化大肠杆菌(Escherichia coli)DH10Bac感受态细胞,经卡那霉素、四环素、庆大霉素及蓝白斑筛选得到重组杆状病毒载体pBac6His11ReGFP,PCR鉴定得到单一目的基因条带。将该病毒载体转染Sf9细胞,进行病毒包装,在倒置荧光显微镜下观察蛋白表达,并以荧光定量PCR方法测定病毒滴度。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2009-12-29
基金项目: 国家自然科学基金(81071850)
作者简介: 赵清霞(1983-),女,山东滨州人,硕士研究生,从事生物化学与分子生物学研究。
通讯作者: 钱其军,电子邮箱:qianqj@163.com
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