|本期目录/Table of Contents|

[1]张虹,蒋红亮,赵辅昆,等. 高表达PGA的枯草芽孢杆菌蛋白质组学研究[J].浙江理工大学学报,2011,28(01):116-121.
　ZHANG Hong,JIANG Hong liang,ZHAO Fu kun,et al. Proteomics Analysis of B.S 168 Overproducing PGA[J].Journal of Zhejiang Sci-Tech University,2011,28(01):116-121.
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 高表达PGA的枯草芽孢杆菌蛋白质组学研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
116-121
栏目:
(自科)生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Proteomics Analysis of B.S 168 Overproducing PGA
文章编号:
1673\|3851 (2011) 01\|0116\|06
作者:
 张虹;  蒋红亮;  赵辅昆;  丁明
 浙江理工大学生命科学院， 杭州 310018
Author(s):
 ZHANG Hong;  JIANG Hongliang;  ZHAO Fukun;  DING Ming
 School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; PGA;  MALDITOFTOF;  双向电泳;  蛋白质组
分类号:
Q784
文献标志码:
A
摘要:
    为了解外源蛋白高表达时枯草芽孢杆菌(Bacillus subtilis)表达系统胞内蛋白质组的变化，实验建立了青霉素G酰化酶 (Penicillin G Acylase，PGA) 高表达B.subtilis系统，可达到1.6 g/L的表达量以及10 U/mL 的酶活；通过双向电泳技术（2DE）分离PGA高表达以及低表达重组菌中胞内蛋白，软件分析结果显示，两者胞内蛋白表达差异极大；通过MALDITOFTOF 质谱鉴定出其中6个差异蛋白，其中4个与PGA高表达抑制菌体生长相关，而PhoR和YxiE则与PGA高表达分泌胁迫机制有关。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2010-01-19
作者简介： 张虹（1985-），女，浙江绍兴人，硕士研究生，主要从事生物化学与分子生物学的研究。
通讯作者： 丁明，电子邮箱：mingding@zstu.edu.cn
更新日期/Last Update: