|本期目录/Table of Contents|

[1]梁宗锁,杨东风,杨宗岐,等.不同土壤水分对丹参叶片总酚酸类成分积累及相关酶活性的影响[J].浙江理工大学学报,2013,30(04):573-578.
 LIANG Zongguo,YANG Dongfeng,YANG Zongqi,et al.Effect of Different Soil Moistures on Total Phenolic Acid Composition Accumulation and Relevant Enzymatic Activity of Salvia Miltiorrhiza Lamina[J].Journal of Zhejiang Sci-Tech University,2013,30(04):573-578.
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不同土壤水分对丹参叶片总酚酸类成分积累及相关酶活性的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年04期
页码:
573-578
栏目:
(自科)生物与生命科学
出版日期:
2013-07-10

文章信息/Info

Title:
Effect of Different Soil Moistures on Total Phenolic Acid Composition Accumulation and Relevant Enzymatic Activity of Salvia Miltiorrhiza Lamina
文章编号:
1673-3851 (2013) 04-0573-06
作者:
梁宗锁 杨东风 杨宗岐 韩蕊莲 刘晓蕾
1. 浙江理工大学生命学院, 杭州 310018; 2. 西北农林科技大学生命科学院, 陕西杨凌 712100
Author(s):
LIANG Zongguo YANG Dongfeng YANG Zongqi HAN Ruilian LIU Xiaolei
1. School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China;2. School of Life Science, Northwest A & F University, Yangling 712100, China
关键词:
土壤水分 总酚酸 丹参 酶活性
分类号:
R282.2
文献标志码:
A
摘要:
以丹参(Salvia miltiorrhiza Bunge)为材料,采用盆栽实验,研究丹参叶片在不同土壤水分(田间持水量的35%、55%、75%)条件下总酚酸类成分积累及相关酶活性的变化规律。结果表明干旱胁迫后期,丹参叶片总酚酸类成分积累明显提高,表现为总酚酸含量以55%处理最高,35%处理次之,75%处理最低,其中55%处理总酚酸含量达到75%处理的1.50倍,35%处理达到75%处理的1.31倍。处理前期,55%土壤含水量能够促进PAL酶活性提高,而处理中后期55%和35%土壤含水量均能够促进TAT酶活性提高,但干旱胁迫对C4H酶活性影响不显著。丹参叶片总酚酸类成分的积累与PAL和C4H酶相关,未发现与TAT有明显关系。这为丹参叶片资源的开发和丹参规范化种植与灌溉提供了理论参考,也为丹参药材的质量控制提供了指导。

参考文献/References:

[1] Pan H J, Li D Y, Fang F, et al. Salvianolic acid a demonstrates cardioprotective effects in rat hearts and cardiomyocytes after ischemia/reperfusion injury[J]. Journal of Cardiovascular Pharmacology, 2011, 58(5): 535-542.
[2] Cao W, Guo X W, Zheng H Z, et al. Current progress of research on pharmacologic actions of salvianolic acid B[J]. Chinese Journal of Integrative Medicine, 2012, 18(4): 316-320.
[3] Zhang Y A, Li X, Wang Z Z. Antioxidant activities of leaf extract of Salvia miltiorrhiza Bunge and related phenolic constituents[J]. Food and Chemical Toxicology, 2010, 48(10): 2656-2662.
[4] Dixon R A, Paiva N L. Stressinduced phenylpropanoid metabolism[J]. The Plant Cell, 1995, 7(7): 1085-1097.
[5] Zhao J, Davis L C, Verpoorte R. Elicitor signal transduction leading to production of plant secondary metabolites[J]. Biotechnology Advances, 2005, 23(4): 283-333.
[6] Sgarbi E, Fornasiero R B, Lins A P, et al. Phenol metabolism is differentially affected by ozone in two cell lines from grape(Vitis vinifera L. ) leaf[J]. Plant Science, 2003, 165(5): 951-957 .
[7] Solecka D, Kacperska A. Phenylpropanoid deficiency affects the course of plant acclimation to cold[J]. Physiologia Plantarum, 2003, 119(2): 253-262 .
[8] Razzaque A, Ellis B E. Rosmarinic acid production in Coleus cell cultures[J]. Planta, 1997, 137(3): 287-291.
[9] 焦蒙丽, 曹蓉蓉, 陈红艳, 等. 水杨酸对丹参培养细胞中迷迭香酸生物合成及其相关酶的影响[J]. 生物工程学报, 2012, 28(3): 320-328.
[10] Dong J A, Wan G W, Liang Z S. Accumulation of salicylic acidinduced phenolic compounds and raised activities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell cultures[J]. Journal of Biotechnology, 2010, 148(2-3): 99-104.
[11] Zhu Z B, Liang Z S, Han R L. Saikosaponin accumulation and antioxidative protection in drought stressed Bupleurum chinense DC. plants[J]. Environment Experimantal Botany, 2009, 66(2): 326-333.
[12] Wang D H, Du F, Liu H Y, et al. Drought stress increases iridoid glycosides biosynthesis in the roots of  Scrophularia ningpoensisseedlings[J]. Journal of Medicinal Plants Research, 2010, 4(24 ): 2691-2699.
[13] 金丽萍, 崔世茂, 杜金伟. 干旱胁迫对不同生态条件下蒙古扁桃叶片 PAL和C4H活性的影响[J]. 华北农学, 2009, 24(5): 118-122.
[14] 田树革, 魏玉龙, 刘宏炳. Folin Ciocalteu比色法测定石榴不同部位总多酚的含量[J]. 光谱实验室, 2009, 26(2): 341-344.
[15] 李倩. 光质对生菜、丹参生长和次生代谢物的影响[D]. 陕西杨陵: 西北农林科技大学, 2010: 69-70.
[16] 孙群, 胡景江. 植物生理学研究技术[M]. 陕西杨陵: 西北农林科技大学出版社, 2006: 178-179.
[17] Hartmann T. From waste products to ecochemicals: fifty years research of plant secondary metabolism[J]. Phytochemistry, 2007, 68(22-24): 2831-2846.
[18] Oh M M, Trick H N, Rajashekar C B. Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce[J]. Journal of Plant Physiology, 2009, 166(2): 180-191.
[19] Liu H, Wang X, Wang D, et al. Effect of drought stress on growth and accumulation of active constituents in Salvia miltiorrhiza Bunge[J]. Industrial Crops and Product, 2011, 33(1): 84-88.
[20] Yang D, Sheng D, Duan Q, et al. PEG and ABA trigger the burst of reactive oxygen species to increase tanshinone production in Salvia miltiorrhiza hairy roots[J]. Journal of Plant Growth Regulation, 2012, 31(4): 579-587.
[21] Yang D, Ma P, Liang X, et al.(2012) PEG and ABA trigger methyl jasmonate accumulation to induce the MEP pathway and increase tanshinone production in Salvia miltiorrhiza hairy roots[J]. Physiologia Plantarum, 2012, 146(2): 1399-3054.
[22] Hernandez I, Alegre L, Munne Bosch S, Drought induced changes in flavonoids and other low molecular weight antioxidants in Cistus clusii grown under mediterranean field conditions[J]. Tree Physiology, 2004, 24(11): 1303-1311.
[23] Beckman C H. Phenolic storing cells: keys to Programmed cell death and periderm formation in wilt disease resistance and in general defence rsponses in plant[J]. Physiological and Molecular Plant Pathology, 2000, 57(3): 101-110.

备注/Memo

备注/Memo:
收稿日期: 2012-10-12
基金项目: 浙江理工大学科研启动基金(1204806Y,1204822 Y);高等学校博士学科点专项科研基金(20110204110028)
作者简介: 梁宗锁(1965-),男,陕西扶风人,教授,博士生导师,主要从事药用植物次生代谢调控和中草药规范化栽培的理论与技术研究。
通信作者: 韩蕊莲,教授,电子邮箱:ruilianxiao@yahoo.com.cn
更新日期/Last Update: 2013-10-24