|本期目录/Table of Contents|

[1]张珊珊,吴金光,胡秀芳. 土壤类芽孢杆菌PCR鉴定方法的建立[J].浙江理工大学学报,2012,29(06):852-857.
　ZHANG Shan shan,WU Jin guang,HU Xiu fang. Development of a PCR Identification Method forPaenibacillus edaphicus[J].Journal of Zhejiang Sci-Tech University,2012,29(06):852-857.
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 土壤类芽孢杆菌PCR鉴定方法的建立()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年06期
页码:
852-857
栏目:
(自科)生物与生命科学
出版日期:
2012-12-28

文章信息/Info

Title:
 Development of a PCR Identification Method forPaenibacillus edaphicus
文章编号:
16733851 (2012) 06085206
作者:
 张珊珊;  吴金光;  胡秀芳
 浙江理工大学生物工程研究所， 杭州 310018
Author(s):
 ZHANG Shanshan;  WU Jinguang;  HU Xiufang
 Institute of Bioengineering, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; 土壤类芽孢杆菌;  gyr B基因;  PCR鉴定;  特异性;  灵敏性
分类号:
Q939.3
文献标志码:
A
摘要:
    B基因为靶标，设计土壤类芽孢杆菌的特异性引物，建立了土壤类芽孢杆菌的特异性PCR鉴定方法。该方法具有较高的特异性，可有效区分胶质类芽孢杆菌及其它各种细菌菌种。该PCR方法也具有较好的灵敏度，可检测只含1到10细胞之间的检测样品。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2012-03-15
基金项目： 国家“863”项目（2006AA10Z428）
作者简介： 张珊珊（1985-），女，硕士研究生，浙江温岭人，主要从事土壤微生物分子生物学研究。
通信作者：胡秀芳，电子邮箱:huxiuf@sina.com
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