|本期目录/Table of Contents|

[1]吴少敏,顾周杭.基于RNA3缺陷型干扰RNA抑制CMV积累的功能分析[J].浙江理工大学学报,2026,55-56(自科六):795-802.
　WU Shaomin,GU Zhouhang.Functional analysis of the defective interfering RNA based on RNA3 in inhibiting CMV accumulation[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科六):795-802.
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基于RNA3缺陷型干扰RNA抑制CMV积累的功能分析()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第六期
页码:
795-802
栏目:
出版日期:
2026-11-10

文章信息/Info

Title:
Functional analysis of the defective interfering RNA based on RNA3 in inhibiting CMV accumulation
文章编号:
1673-3851(2026) 11-0795-08
作者:
吴少敏; 顾周杭
浙江理工大学生命科学与医药学院 ,杭州 310018
Author(s):
WU Shaomin; GU Zhouhang
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
本氏烟; 黄瓜花叶病毒; 亚基因组; 缺陷型 RNA3; 基因组RNA含量
分类号:
Q939.46
文献标志码:
A
摘要:
为探究人工构建的黄瓜花叶病毒(Cucumbermosaicvirus, CMV)缺陷型干扰 RNA是否具有抑制 CMV积累的功能 ,以 CMV的基因组 RNA3为研究对象 ,分别构建了缺失运动蛋白基因(Movementprotein, MP) 、外壳蛋白基因 (Coatprotein, CP)以及 MP/CP双缺失的缺陷型 RNA3侵染性克隆 ,并分析上述缺陷型 RNA3抑制 CMV-eGFP积累的能力 。结果表明 :单独缺失 MP或 CP的缺陷型 RNA3均能有效干扰 CMV-eGFP积累 ,并降低病毒编码的 eGFP表达量 ,相比于单独缺失 MP的缺陷型 RNA3,单独缺失 CP的缺陷型RNA3表现出更强的抑制效果;2种单缺失突变体对 CMV的抑制作用均不依赖于 RDR6介导的基因沉默途径;MP/CP双缺失突变体 RNA3因不能正常复制或复制水平极低 ,丧失了抑制 CMV-eGFP积累的功能 。该研究为基于缺陷型RNA分子防控 CMV提供理论依据与技术支撑。

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备注/Memo

备注/Memo:
基金项目 : 国家自然科学基金项目(32172047)收稿日期 : 2026-03-26 网络出版日期 : 2026-06-04
作者简介 : 吴少敏 (2000— ) ,女 ,福建莆田人 ,硕士研究生 ,主要从事植物病毒学方面的研究。通信作者: 顾周杭,E-mail:hzgu@zstu. edu. cn0 引 言
更新日期/Last Update: 2026-11-09