|本期目录/Table of Contents|

[1]应悦,柴新想,刘轩,等.壳聚糖基润滑液的制备及促进骨关节炎软骨修复作用研究[J].浙江理工大学学报,2026,55-56(自科四):404-411.[doi:org/10.14336/AD. 2024.108 0]
　YINGYue,CHAIXinxiang,LIUXuan,et al.A study on the preparation of chitosan-based lubricant for promoting cartilage repair in osteoarthritis[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科四):404-411.[doi:org/10.14336/AD. 2024.108 0]
点击复制

壳聚糖基润滑液的制备及促进骨关节炎软骨修复作用研究()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第四期
页码:
404-411
栏目:
出版日期:
2026-07-10

文章信息/Info

Title:
A study on the preparation of chitosan-based lubricant for promoting cartilage repair in osteoarthritis
文章编号:
1673-3851(2026) 07-0404-08
作者:
应悦; 柴新想; 刘轩; 江国华
浙江理工大学材料科学与工程学院 ,杭州 310018
Author(s):
YINGYue; CHAIXinxiang; LIUXuan; JIANGGuohua
School of Materials Science& Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
壳聚糖; 贻贝粘蛋白 ; 润滑液 ; 摩擦; 骨关节炎 ; 软骨
分类号:
TB33
DOI:
org/10.14336/AD. 2024.108 0
文献标志码:
A
摘要:
为克服临床润滑液作为骨关节炎治疗粘液补充剂仅具抗磨效果的局限 , 以壳聚糖、甲基丙烯酸酐、精氨酸、贻贝粘蛋白(Musseladhesiveprotein,MAP)及双氯芬酸钠(Diclofenacsodium,DS)为主要材料 ,设计了一种具有抗炎性能且摩擦学特性略优于临床润滑液的复合润滑液系统 ,并通过组织切片染色和免疫组化染色评估其在骨关节炎治疗中的应用价值 。 结果表明 : 当精氨酸共轭甲基丙烯酰化壳聚糖 (Arginine-conjugated methacryloylated chitosan,CMA)与 MAP的质量浓度比为 10∶1时 ,在 0.1~ 1000.0 s-1 的剪切速率下 ,CMA/MAP的黏度从 10.40 Pa ·s降至0.12Pa·s,摩擦系数显著低于医用玻璃酸钠;DS功能化后的复合润滑液 DS@CMA/MAP在体外实验中表现出优异的生物相容性 ,与不同浓度复合润滑液共培养的细胞活力均大于 90% ,并呈现增殖趋势 ,划痕实验中 DS @CMA/MAP组迁移效率提升 37.02%;DS@CMA/MAP复合润滑液能够显著抑制炎症反应并促进软骨修复 ,其TNF-α 阳性率为假手术组的 245.32% ,Aggrecan覆盖率达到假手术组的 50.83% 。该研究开发的 DS@CMA/MAP复合润滑液在维持良好抗摩擦性能的同时 ,兼具优异的生物相容性、促细胞迁移能力、抗炎及促进软骨修复功能 , 为骨关节炎的综合治疗提供了新的策略。

参考文献/References:

[1] Tang S, Zhang C, Oo W M, et al. Osteoarthritis[J]. Nat Rev Dis Primers, 2025, 11(1): 10.
[2] Shen P, Löhning M. Insights into osteoarthritis development from single-cell RNA sequencing of subchondral bone[J]. RMD Open, 2022, 8(2): e002617.
[3] Hunter D J, Bierma-Zeinstra S. Osteoarthritis[J]. The Lancet, 2019, 393(10182): 1745‑1759.
[4] Allen K D, Woolson S, Hoenig H M, et al. Stepped exercise program for patients with knee osteoarthritis: a randomized controlled trial[J]. Annals of Internal Medicine, 2021, 174(3): 298‑307.
[5] Slomski A. Physical therapy outperforms injections for knee osteoarthritis[J]. JAMA, 2020, 323(24): 2453.
[6] Walker A, Sibley F, Carter A, et al. Social return on investment analysis of a physiotherapy-led service for managing osteoarthritis in primary care[J]. The Lancet, 2017, 389: S98.
[7] Richard M J, Driban J B, McAlindon T E. Pharmaceutical treatment of osteoarthritis[J]. Osteoarthritis and Cartilage, 2023, 31(4): 458‑466.
[8] Laslett L L, Scheepers L E J M, Antony B, et al. Krill oil for knee osteoarthritis: a randomized clinical trial[J]. JAMA, 2024, 331(23): 1997‑2006.
[9] Neogi T, Dubreuil M, Peloquin C, et al. Association of physical therapy care with use of intra-articular injections in people with knee osteoarthritis: a real-world cohort study[J]. Arthritis & Rheumatology, 2025, 77(8): 1006‑1014.
[10] Dubreuil M, Louie-Gao Q, Peloquin C E, et al. Risk of myocardial infarction with use of selected non-steroidal anti-inflammatory drugs in patients with spondyloarthritis and osteoarthritis[J]. Annals of the Rheumatic Diseases, 2018, 77(8): 1137‑1142.
[11] Bindu S, Mazumder S, Bandyopadhyay U. Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: a current perspective[J]. Biochemical Pharmacology, 2020, 180: 114147.
[12] Qiu S, Shi Y, Zang H, et al. Multifunctional injectable microspheres for osteoarthritis therapy via spatiotemporally modulating macrophage polarization and inflammation[J]. NPJ Regenerative Medicine, 2024, 9: 22.
[13] Zhang Y, Chen X, Tong Y, et al. Development and prospect of intra-articular injection in the treatment of osteoarthritis: a review[J]. Journal of Pain Research, 2020, 13: 1941‑1955.
[14] Tan H, Chu C R, Payne K A, et al. Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for cartilage tissue engineering[J]. Biomaterials, 2009, 30(13): 2499‑2506.
[15] Meng T, Zhao Z, Song M, et al. Carboxybetaine modified chitosan as viscosupplementation for osteoarthritis therapy[J]. International Journal of Biological Macromolecules, 2025, 307: 142155.
[16] Zhao X, Yang L, Zhang L, et al. Novel biomimetic macromolecule system for highly efficient lubrication, ROS scavenging and osteoarthritis treatment[J]. Colloids and Surfaces B: Biointerfaces, 2024, 239: 113956.
[17] Scognamiglio F, Travan A, Donati I, et al. A hydrogel system based on a lactose-modified chitosan for viscosupplementation in osteoarthritis[J]. Carbohydrate Polymers, 2020, 248: 116787.
[18] Fan X, Chen G, Wang S, et al. Applications of chitosan and its derivatives in the treatment of osteoarthritis[J/OL]. Aging and Disease, 2024. (2024-12-16)[2025-05-07]. https://doi.org/10.14336/AD.2024.1080.
[19] Xu P, Chen P, Sun Y, et al. A novel injectable thermo/photo dual-crosslinking hydrogel based on modified chitosan for fast sealing open globe injury[J]. Carbohydrate Polymers, 2024, 331: 121854.
[20] Liu W G, Zhang J R, Cao Z Q, et al. A chitosan-arginine conjugate as a novel anticoagulation biomaterial[J]. Journal of Materials Science: Materials in Medicine, 2004, 15(11): 1199‑1203.
[21] Huang L, Wang W, Xian Y, et al. Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair[J]. Materials Today Bio, 2023, 22: 100752.
[22] Hanafy A S, El-Ganainy S O. Thermoresponsive Hyalomer intra-articular hydrogels improve monoiodoacetate-induced osteoarthritis in rats[J]. International Journal of Pharmaceutics, 2020, 573: 118859.
[23] Ghosh S. Metabolomic studies for metabolicalterations induced by non-steroidal anti-inflammatory drugs: Minireview[J]. Biomolecules, 2021, 11(10): 1456.

相似文献/References:

[1]徐寅a,王家俊b,刘幸幸a,等. 纳米纤维素晶须/壳聚糖天然可降解复合膜的制备与性能[J].浙江理工大学学报,2011,28(06):841.
　XU Yina,WANG Jia junb,LIU Xing xinga,et al. Preparation and Properties of Nanocellulose Whiskers/Chitosan Natural Biodegradable Composite Films[J].Journal of Zhejiang Sci-Tech University,2011,28(自科四):841.
[2]郭丽a,王家俊b,樊春艳a,等. 层状双金属氢氧化物/壳聚糖复合膜的制备与性能研究[J].浙江理工大学学报,2011,28(06):860.
　GUO Lia,WANG Jia junb,FAN Chun yana,et al. Study on the Preparation and Properties of MLDHs/Chitosan Hybrid Films[J].Journal of Zhejiang Sci-Tech University,2011,28(自科四):860.
[3]陈鹏,王耐艳,郑莹莹.聚氧化乙烯@明胶纤维制备及其生物性能探究[J].浙江理工大学学报,2019,41-42(自科一):56.
　CHEN peng,WANG Naiyan,ZHENG Yingying.Preparation and biological properties of Chitosan PEO@Gelatin nanofibers[J].Journal of Zhejiang Sci-Tech University,2019,41-42(自科四):56.
[4]刘乐云,冯诗璇,王耐艳,等.锶磷灰石@壳聚糖聚氧化乙烯@明胶复合支架制备及其生物性能探究[J].浙江理工大学学报,2019,41-42(自科六):776.
　LIU Leyuna,FENG Shixuana,WANG Naiyanb,et al.Preparation and biological properties of Strontium hydroxyapatite@ChitosanPEO@Gelatin composite scaffold[J].Journal of Zhejiang Sci-Tech University,2019,41-42(自科四):776.
[5]王莉,刘涛,丁新波,等.丝素-壳聚糖/聚氧化乙烯双层纳米纤维膜制备及其生物活性[J].浙江理工大学学报,2022,47-48(自科四):474.
　WANG Li,LIU Tao,DING Xinbo,et al.Preparation of silk fibroin chitosan/polyvinyl oxide  bilayer nanofiber membrane and its bioactivity[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科四):474.
[6]夏月,孙辉,于斌.ZnO/CS@M-PP复合熔喷材料的制备及其抗菌抗紫外性能[J].浙江理工大学学报,2023,49-50(自科六):674.
　XIA Yue,SUN Hui,YU Bin.Preparation of ZnO/CS@M-PP composite meltblown materials and their antibacterial and UV resistance properties[J].Journal of Zhejiang Sci-Tech University,2023,49-50(自科四):674.
[7]谢冉,李彬.一氧化氮释放壳聚糖复合水凝胶的制备及其抗菌性能评估[J].浙江理工大学学报,2024,51-52(自科六):753.
　XIE Ran,LI Bin.Preparation of a nitric oxide releasing chitosan based composite hydrogel and assessment of its antimicrobial properties[J].Journal of Zhejiang Sci-Tech University,2024,51-52(自科四):753.

备注/Memo

备注/Memo:
收稿日期 : 2025-05-07 网络出版日期 : 2025-10-09作者简介 : 应 悦 (2000— ) ,女 ,浙江金华人 ,硕士研究生 ,主要从事生物医用材料方面的研究。通信作者: 江国华,E-mail:ghjiang_cn@zstu. edu. cn
更新日期/Last Update: 2026-07-05