|本期目录/Table of Contents|

[1]刘昕彤,黄焕会,舒建洪,等.猪流行性腹泻病毒的多表位 mRNA疫苗设计与免疫评价[J].浙江理工大学学报,2026,55-56(自科二):198-206.
　LIU Xintong,HUANG Huanhui,SHU Jianhong,et al.Design and immunological evaluation of a multi-epitope mRNA vaccine againstporcine epidemic diarrhea viru[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科二):198-206.
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猪流行性腹泻病毒的多表位 mRNA疫苗设计与免疫评价()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第二期
页码:
198-206
栏目:
出版日期:
2026-03-15

文章信息/Info

Title:
Design and immunological evaluation of a multi-epitope mRNA vaccine againstporcine epidemic diarrhea viru
文章编号:
1673-3851(2026) 03-0198-09
作者:
刘昕彤 ; 黄焕会 ; 舒建洪 ; 金伟波
1. 浙江理工大学生命科学与医药学院 ,杭州 310018;2. 浙江理工大学绍兴生物医药研究院 ,浙江绍兴 312366
Author(s):
LIU Xintong ; HUANG Huanhui ; SHU Jianhong; JIN Weibo
1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Shaoxing Academy of Biomedicine, Zhejiang Sci-Tech University, Shaoxing312366, China
关键词:
猪流行性腹泻病毒; 多表位mRNA疫苗; 免疫信息学; 抗原表位; 免疫原性
分类号:
S858.28
文献标志码:
A
摘要:
为解决猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV) 现有疫苗保护力不足的问题 , 结合 免疫信息学与 mRNA技术 ,设计并构建一种基于多表位抗原(multi-epitopeantigen,ME) 的新型疫苗 。利用 VaxiJen 和 ANTIGENpro抗原性预测工具 , 以从 PEDV结构蛋白中筛选的 S蛋白(Q91AV1) 和 N蛋白(Q07499) 为候选抗 原 ,分别采用 IEDB、ABCpred和 NetMHCpan软件预测了 25条 B细胞优势表位和3条 T细胞优势表位 ,并通过柔性 连接肽将其串联整合 , 引入 Foldon三聚体结构及分泌信号肽构建 ME序列; 采用 mRNA-LNP递送系统制备 ME mRNA疫苗 ,并通过小鼠免疫实验评估该疫苗的免疫原性 。结果显示 :ME/S1mRNA联合免疫组在小鼠首次免疫 后 ,其 PEDV特异性免疫球蛋白 G(Immunoglobulin G, IgG) 抗体水平 OD450 nm 值为 1.250±0.068, 显著高于 S1 mRNA单免疫组 OD450 nm 值(0.816±0.074) 和传统灭活疫苗组 OD450 nm 值(0.805±0.065);ME/S1mRNA联合免 疫组在小鼠加强免疫后 ,联合组抗体滴度进一步提升 ,其 OD450 nm 值为 1.681±0.100,仍显著高于单免疫组 OD450 nm 值(1.238±0.074) 与传统灭活疫苗组 OD450 nm 值(1.164±0.114);ME与 S1mRNA联合免疫的多表位设计可通过 协同激发 B/T细胞应答 ,增强PEDV免疫效果 。该文结果为 PEDV防控提供了高效候选策略 ,并为其他冠状病毒 类动物疫苗的设计与开发提供了技术参考。

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相似文献/References:

[1]张秋桠,刘高鹏,刘芹,等.猪流行性腹泻病毒RT-PCR检测方法的建立[J].浙江理工大学学报,2022,47-48(自科四):629.
　ZHANG Qiuya,LIU Gaopeng,LIU Qin,et al.Establishment of a RTPCR detection method for porcine epidemic diarrhea virus[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科二):629.

备注/Memo

备注/Memo:
收稿日期 : 2025-04-07 网络出版日期 : 2025-11-03基金项目 : 国家自然科学基金项目(32172496)作者简介 : 刘昕彤(1999— ) ,女 ,辽宁朝阳人 ,硕士研究生 ,主要从事 AI辅助药物发现方向的研究。通信作者 : 金伟波 ,E-mail:jwb@zstu. edu. cn
更新日期/Last Update: 2026-03-06