|本期目录/Table of Contents|

[1]孙佳怡,鲁晓锋,舒建洪,等.聚乙烯亚胺纳米载体的设计及其在DNA体内外递送中的性能评估[J].浙江理工大学学报,2025,53-54(自科五):656-663.
　SUN Jiayi,LU Xiaofeng,SHU Jianhong,et al.Design of polyethyleneimine nanocarriers and evaluation of  their performance in DNA delivery  in vivo  and  ex vivo[J].Journal of Zhejiang Sci-Tech University,2025,53-54(自科五):656-663.
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聚乙烯亚胺纳米载体的设计及其在DNA体内外递送中的性能评估()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第53-54卷
期数:
2025年自科第五期
页码:
656-663
栏目:
出版日期:
2025-09-10

文章信息/Info

Title:
Design of polyethyleneimine nanocarriers and evaluation of  their performance in DNA delivery  in vivo  and  ex vivo
文章编号:
1673-3851 (2025) 09-0656-08
作者:
孙佳怡; 鲁晓锋; 舒建洪; 吴方丽; 金伟波
 1.浙江理工大学生命科学与医药学院，杭州 310018；2.浙江理工大学绍兴生物医药研究院，浙江绍兴 312366
Author(s):
SUN Jiayi; LU Xiaofeng; SHU Jianhong; WU Fangli; JIN Weibo
1.College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China;2.Zhejiang SciTech University Shaoxing Academy of Biomedicine, Shaoxing 312366, China
关键词:
聚乙烯亚胺; 二油酰基磷脂酰乙醇胺; 胆固醇; PEI/DNA纳米颗粒; DNA递送
分类号:
Q936
文献标志码:
A
摘要:
聚乙烯亚胺（Polyethyleneimine, PEI）纳米载体广泛应用于核酸药物的递送，但高细胞毒性限制了其在临床上的应用。采用二油酰基磷脂酰乙醇胺（Dioleoyl phosphatidylethanolamine, DOPE）和胆固醇修饰分子量为1.2 kDa与25.0 kDa的PEI，并将修饰后的PEI与DNA按照不同的氮磷比混合，制备PEI/DNA纳米颗粒；评估纳米颗粒的理化性质、细胞毒性与转染效率，筛选高效低毒的PEI纳米载体。结果显示：DOPE和胆固醇修饰显著降低了PEI/DNA纳米颗粒的细胞毒性。当氮磷比为3和5时，修饰后的纳米颗粒的平均粒径约为200 nm，Zeta电位在5 mV至10 mV之间，分散度在0.1至0.2之间；当氮磷比为5时，修饰后的25.0 kDa纳米颗粒展现出最高的DNA体内外递送效率和低细胞毒性。该结果表明辅助脂质修饰显著影响了纳米颗粒的细胞毒性，PEI分子量和氮磷比显著影响了纳米颗粒的理化性质和转染效率。筛选出修饰后的25.0 kDa PEI在氮磷比为5时与DNA混合制备的纳米颗粒兼具高效的基因递送效率和良好的生物相容性，为PEI纳米载体在DNA疫苗递送领域的应用提供了数据支撑。

参考文献/References:

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［6］Ahmadi Z, Jha D, Kumar B, et al. Bifunctionally engineered polyethylenimines as efficient DNA carriers and antibacterials against resistant pathogens［J］. Journal of Biomaterials Applications, 2018, 33(3): 363-379.
［7］Clark S R, Lee K Y, Lee H, et al. Determining the effects of PEI adsorption on the permeability of 1,2-dipalmitoylphosphatidylcholine/bis(monoacylglycero)phosphate membranes under osmotic stress［J］. Acta Biomaterialia, 2018, 65: 317-326.
［8］Beyerle A, Irmler M, Beckers J, et al. Toxicity pathway focused gene expression profiling of PEI-based polymers for pulmonary applications［J］. Molecular Pharmaceutics, 2010, 7(3): 727-737.
［9］Lee Y, Miyata K, Oba M, et al. Charge-conversion ternary polyplex with endosome disruption moiety: A technique for efficient and safe gene delivery［J］. Angewandte Chemie International Edition, 2008, 120(28): 5241-5244.
［10］Wu P, Luo X, Wu H, et al. Combined hydrophobization of polyethylenimine with cholesterol and perfluorobutyrate improves siRNA delivery［J］. Bioconjugate Chemistry, 2020, 31(3): 698-707.

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备注/Memo

备注/Memo:
收稿日期： 2025-03-03
网络出版日期：2025-06-26
基金项目： 国家自然科学基金面上项目（32172496）
作者简介： 孙佳怡（1999—），女，江苏苏州人，硕士研究生，主要从事纳米载体递送方面的研究
通信作者： 金伟波，E-mail：jwb@zstu.edu.cn
更新日期/Last Update: 2025-09-15