|本期目录/Table of Contents|

[1]王洁瑶,马蓉,邢孔丫,等.基于转录组学的拟南芥 AtNDX 下游潜在靶基因挖掘与分析[J].浙江理工大学学报,2025,53-54(自科五):646-655.
　WANG Jieyao,MA Rong,XING Kongya,et al.Mining and analysis of potential target genes downstream of AtNDX in  Arabidopsis thaliana based on transcriptomics[J].Journal of Zhejiang Sci-Tech University,2025,53-54(自科五):646-655.
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基于转录组学的拟南芥 AtNDX 下游潜在靶基因挖掘与分析()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第53-54卷
期数:
2025年自科第五期
页码:
646-655
栏目:
出版日期:
2025-09-10

文章信息/Info

Title:
Mining and analysis of potential target genes downstream of AtNDX in  Arabidopsis thaliana based on transcriptomics
文章编号:
1673-3851 (2025) 09-0646-10
作者:
王洁瑶; 马蓉; 邢孔丫; 柯丽萍; 华学军
浙江理工大学生命科学与医药学院，杭州 310018
Author(s):
WANG Jieyao; MA Rong; XING Kongya; KE Liping; HUA Xuejun
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
拟南芥; 转录组; AtNDX; 下游靶基因; 线粒体胁迫
分类号:
Q756
文献标志码:
A
摘要:
植物线粒体在受到环境胁迫时会产生逆向信号，通过调节核基因的表达来协调植物生长和抗逆的平衡，但其内在作用机制还有待深入研究。首先对拟南芥SSR1基因突变体（ssr1 2）、ssr1 2 抑制子（ sus3）、sus3回补株系（ sus3C ）及野生型Wassilewskija（WS）植株的根进行转录组测序；其次利用主成分分析和Pearson相关系数评估转录组数据之间的相关性，绘制差异表达基因的火山图和相关性聚类分析热图；最后结合KEGG和GO分析，筛选同时受线粒体胁迫和AtNDX调控的基因，分析AtNDX在线粒体胁迫下调控植物生长发育的作用机制。结果表明：4个材料的高通量测序数据重复性好，且ssr1 2与sus3C 高度相关， sus3C 能互补 sus3抑制子突变造成的影响；筛选到线粒体胁迫下受 AtNDX 负调控的基因有288个，受AtNDX正调控的基因有178个；利用实时荧光定量PCR技术对筛选出的15个在线粒体胁迫下受AtNDX调控的潜在下游靶基因进行了验证，发现这些基因均在植物生长发育和细胞代谢过程中发挥重要作用。该结果表明，AtNDX通过调控根系发育和细胞代谢相关基因的表达参与线粒体逆向信号的响应和植物生长发育调节，为深入解析植物线粒体在平衡生长发育和胁迫响应中的作用提供了重要线索。

参考文献/References:

［1］Noctor G, Paepe R D, Foyer C H. Mitochondrial redox biology and homeostasis in plants［J］. Trends in Plant Science, 2007, 12(3): 125-134.
［2］Sweetlove L J, Fait A, Nunes-Nesi A, et al. The mitochondrion: An integration point of cellular metabolism and signalling［J］. Critical Reviews in Plant Sciences, 2007, 26(1): 17-43.
［3］Millar A H, Whelan J, Soole K L, et al. Organization and regulation of mitochondrial respiration in plants［J］. Annual Review of Plant Biology, 2011, 62: 79-104.
［4］Cagin U, Enriquez J A. The complex crosstalk between mitochondria and the nucleus: What goes in between［J］. The International Journal of Biochemistry & Cell Biology, 2015, 63: 10-15.
［5］Wang Y, Selinski J, Mao C, et al. Linking mitochondrial and chloroplast retrograde signalling in plants［J］. Philosophical Transactions of the Royal Society of London. B. Biological Sciences, 2020, 375(1801): 20190410.
［6］Jones A W E, Yao Z, Vicencio J M, et al. PGC-1 family coactivators and cell fate: roles in cancer, neurodegeneration, cardiovascular disease and retrograde mitochondria-nucleus signalling［J］. Mitochondrion, 2012, 12(1): 86-99.
［7］Granat L, Hunt R J, Bateman J M. Mitochondrial retrograde signalling in neurological disease［J］. Philosophical Transactions of the Royal Society of London. B. Biological Sciences, 2020, 375(1801): 20190415.
［8］Ng S, Giraud E, Duncan O, et al. Cyclin-dependent kinase E1 (CDKE1) provides a cellular switch in plants between growth and stress responses［J］. Journal of Biological Chemistry, 2013, 288(5): 3449-3459.
［9］Blanco N E, Guinea-Díaz M, Whelan J, et al. Interaction between plastid and mitochondrial retrograde signalling pathways during changes to plastid redox status［J］. Philosophical transactions of the Royal Society of London. B. Biological Sciences, 2014, 369(1640): 20130231.
［10］Giraud E, Van Aken O, Ho L H M, et al. The transcription factor ABI4 is a regulator of mitochondrial retrograde expression of alternative oxidase 1a［J］. Plant Physiology, 2009, 150(3): 1286-1296.

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备注/Memo

备注/Memo:
收稿日期： 2025-01-22
网络出版日期：2025-05-07
基金项目： 国家自然科学基金项目（32170307）
作者简介： 王洁瑶(1998—)，女，贵州人，硕士研究生，主要从事植物抗逆分子机制方面的研究
通信作者： 华学军，Email：xjhua@zstu.edu.cn;柯丽萍，E-mail:keliping@zstu.edu.cn
更新日期/Last Update: 2025-09-15