|本期目录/Table of Contents|

[1]姚佳怡,顾周杭.NbUNE12与CMV 2a蛋白相互作用并负调控2b缺陷型CMV突变体侵染植物[J].浙江理工大学学报,2025,53-54(自科二):270-275.
　YAO Jiayi,GU Zhouhang.NbUNE12 interacts with CMV 2a protein and negatively regulates  infected plants of a 2b deleted mutant of CMV[J].Journal of Zhejiang Sci-Tech University,2025,53-54(自科二):270-275.
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NbUNE12与CMV 2a蛋白相互作用并负调控2b缺陷型CMV突变体侵染植物()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第53-54卷
期数:
2025年自科第二期
页码:
270-275
栏目:
出版日期:
2025-03-10

文章信息/Info

Title:
NbUNE12 interacts with CMV 2a protein and negatively regulates  infected plants of a 2b deleted mutant of CMV
文章编号:
1673-3851 (2025) 03-0270-06
作者:
姚佳怡; 顾周杭
浙江理工大学生命科学与医药学院，杭州 310018
Author(s):
YAO Jiayi;  GU Zhouhang
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
黄瓜花叶病毒; bHLH转录因子; UNE12; 本氏烟; 抗性
分类号:
Q939-46
文献标志码:
A
摘要:
为了探究黄瓜花叶病毒（Cucumber mosaic virus, CMV）复制酶2a蛋白细胞核定位的生物学意义，以本氏烟UNE12蛋白（NbUNE12）为研究对象，通过双分子荧光互补实验及病毒诱导的基因沉默方法分析该蛋白与2a蛋白的相互作用情况及其在CMV侵染过程中的作用。结果表明：CMV 2a蛋白与NbUNE12蛋白的相互作用主要集中于细胞核中；下调表达NbUNE12增加CMV的2b缺陷型突变体（CMVΔ2b GFP）在植物系统叶的病毒含量，表明NbUNE12蛋白能够与CMV 2a蛋白发生相互作用，并且负调控CMVΔ2b GFP侵染本氏烟。该研究为CMV 2a蛋白在植物细胞核定位的生物学功能进一步研究奠定理论基础。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2024-07-04
网络出版日期：2024-09-13
基金项目： 浙江理工大学基本科研业务费项目(2021Q032)
作者简介： 姚佳怡(1999—)，女，浙江海宁人，硕士研究生，主要从事植物病毒学方面的研究
通信作者： 顾周杭，E-mail：hzgu@zstu.edu.cn
更新日期/Last Update: 2025-03-06