[1]高婷,任珂瑶,张舒婷,等.高产色素和洛伐他汀红曲菌株的筛选、鉴定及发酵条件研究[J].浙江理工大学学报,2024,51-52(自科五):725-733.
GAO Ting,REN Keyao,ZHANG Shuting,et al.Research on the screening, identification, and fermentation conditions of high yield Monascus strains for pigments and lovastatin[J].Journal of Zhejiang Sci-Tech University,2024,51-52(自科五):725-733.
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高产色素和洛伐他汀红曲菌株的筛选、鉴定及发酵条件研究()
GAO Ting,REN Keyao,ZHANG Shuting,et al.Research on the screening, identification, and fermentation conditions of high yield Monascus strains for pigments and lovastatin[J].Journal of Zhejiang Sci-Tech University,2024,51-52(自科五):725-733.
浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]
- 卷:
- 第51-52卷
- 期数:
- 2024年自科第五期
- 页码:
- 725-733
- 栏目:
- 出版日期:
- 2024-09-20
文章信息/Info
- Title:
- Research on the screening, identification, and fermentation conditions of high yield Monascus strains for pigments and lovastatin
- 分类号:
- Q939-9
- 文献标志码:
- A
- 摘要:
- 红曲色素(Monascus pigments, MPs)和洛伐他汀(Lovastatin)是两种具有极高营养、保健和药用价值的红曲菌代谢产物，寻找挖掘高产MPs和洛伐他汀的红曲菌，具有重要实践意义。从土样中分离得到红曲菌A1和A2，在对其进行分类鉴定的基础上，采用分光光度法对MPs的色价和洛伐他汀含量进行测定，并通过单因素和正交试验对发酵培养基进行了优化。结果表明：菌株A1和A2隶属于紫色红曲菌(Monascus purpureus)。在基础培养基中，菌株A1和A2的MPs色价分别为3287.11、2561.30 U/g，洛伐他汀含量为31.35、33.74 μg/mL。通过 L16 (45)正交试验优化培养基，获得产MPs最优配方为乳糖20 g/L、KNO 3 5 g/L、KH 2PO 4 4 g/L、KCl 2 g/L、Na 3C 6H 5O 7 2 g/L，产洛伐他汀最优配方为乳糖20 g/L、KNO 3 15 g/L、KH 2PO 4 2 g/L、KCl 6 g/L、Na 3C 6H 5O 7 .4 g/L。在最优培养基中A1菌株总色价可达15851.98 U/g，提高了3.82倍，洛伐他汀含量为122.96 μg/mL，提高了2.92倍；A2菌株总色价为8933.58 U/g，提高了2.49倍，洛伐他汀含量达198.26 μg/mL，提高了4.88倍。该文为红曲菌资源库提供了优秀潜力菌株，为工业上大规模生产MPs和洛伐他汀奠定了理论基础并提供了实际方案。
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备注/Memo
- 备注/Memo:
-
收稿日期： 2024-03-29
网络出版日期：2024-07-22
基金项目： 企业委托研发项目(19040097-J)
作者简介： 高婷(1996—)，女，安徽安庆人，硕士研究生，主要从事应用微生物学方面的研究
通信作者： 赵洪新，E-mail：bxxbj2003@gmail.com
