|本期目录/Table of Contents|

[1]李伯齐,郭建军,胡万哉,等.基于GA-BPNN优化铁皮石斛多糖提取工艺[J].浙江理工大学学报,2021,45-46(自科五):697-703.
　LI Boqi,GUO Jianjun,HU Wanzai,et al.Optimization of the extraction process of  Dendrobium officinale  polysaccharide based on GABPNN[J].Journal of Zhejiang Sci-Tech University,2021,45-46(自科五):697-703.
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基于GA-BPNN优化铁皮石斛多糖提取工艺()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第45-46卷
期数:
2021年自科第五期
页码:
697-703
栏目:
出版日期:
2021-09-10

文章信息/Info

Title:
Optimization of the extraction process of  Dendrobium officinale  polysaccharide based on GABPNN
文章编号:
1673-3851 (2021) 09-0697-07
作者:
李伯齐; 郭建军; 胡万哉; 梁宗锁; 侯卓妮
浙江理工大学生命科学与医药学院，杭州 310018
Author(s):
LI Boqi;  GUO Jianjun;  HU Wanzai;  LIANG Zongsuo;  HOU Zhuoni
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018
关键词:
BP神经网络; 铁皮石斛; 多糖; 正交设计; 纤维素酶; 木瓜蛋白酶; 还原糖; 超声提取
分类号:
R 284-2
文献标志码:
A
摘要:
为了优化铁皮石斛多糖提取工艺，采用单因素实验考察超声功率、超声时间、纤维素酶浓度、木瓜蛋白酶浓度、液料比和浸提温度等因素的影响，然后采用正交设计试验方法安排实验，通过Matlab建立三层结构的BP神经网络（Back propagation neural network, BPNN），并结合遗传算法（Genetic algorithm,GA）对工艺条件进行全局寻优，获得最佳提取工艺。结果表明：纤维素酶会对铁皮石斛多糖造成破坏，不利于铁皮石斛多糖提取，而木瓜蛋白酶可显著增加对铁皮石斛多糖的提取得率；铁皮石斛多糖的最佳提取工艺为液料比95∶1，超声功率400 W，木瓜蛋白酶浓度1400 U/g，提取温度48 ℃，在此工艺条件下铁皮石斛多糖得率达33-4%，优于普通热水提取236%的得率，得率提升显著,也同时表明BP神经网络模型结合遗传算法能够较好地解决此类非线性求优问题。

参考文献/References:

［1］ 国家药典委员会. 中华人民共和国药典: 一部［M］. 北京: 中国医药科技出版社， 2015:282-283.
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［3］ He T B, Huang Y P, Yang L L, et al. Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale［J］. International Journal of Biological Macromolecules, 2016, 83(2): 34-41.
［4］ Wei W, Feng L, Bao W R, et al. Structure characterization and immunomodulating effects of polysaccharides isolated from Dendrobium officinale［J］. Journal of Agricultural and Food Chemistry, 2016, 64(4): 881-889.
［5］ Hui F L, Ma J J, Fan G, et al. Structural characterisation and immunomodulatory effects of polysaccharides isolated from Dendrobium aphyllum［J］. International Journal of Food Science & Technology, 2018, 53(5): 1185-1194.
［6］ Wang H Y, Li Q M, Yu N J, et al. Dendrobium huoshanense polysaccharide regulates hepatic glucose homeostasis and pancreatic βcell function in type 2 diabetic mice［J］. Carbohydrate Polymers, 2019, 211(1): 39-48.
［7］ Chen H, Nie Q X, Hu J L, et al. Metabolism amelioration of Dendrobium officinale polysaccharide on type II diabetic rats［J］. Food Hydrocolloids, 2020, 102(5): 105582.
［8］ 张玉娜, 王倩文, 张双灵. 水提醇沉法提取香菇多糖的最佳工艺研究［J］. 青岛农业大学学报(自然科学版), 2020, 37(1): 43-46.
［9］ Wu Q, Qin D D, Cao H X, et al. Enzymatic hydrolysis of polysaccharide from Auricularia auricula and characterization of the degradation product［J］. International Journal of Biological Macromolecules, 2020, 162(11): 1-1994.
［10］ Medlej M K, Cherri B, Nasser G, et al. Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their antioxidant properties［J］. International Journal of Biological Macromolecules, 2020, 161(6): 958-968.

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备注/Memo

备注/Memo:
收稿日期：2021-04-01
网络出版日期：2021-05-28
基金项目：浙江省重点研发项目（2016BSA780588）
作者简介：李伯齐（1995-），男，江西上饶人，硕士研究生，主要从事中药现代化方面的研究
通信作者：侯卓妮， E-mail: houzhuoni@zstu.edu.cn
更新日期/Last Update: 2021-09-16