|本期目录/Table of Contents|

[1]黄路瑶,郁吉锋,钱央央,等.铁皮石斛组培苗和栽培苗的circRNA检测及分析[J].浙江理工大学学报,2020,43-44(自科六):840-845.
　HUANG Luyao,YU Jifeng,QIAN Yangyang,et al.Detection and analysis of circRNA in tissue cultured seedlinog and cultivated seedling of Dendrobium officinale[J].Journal of Zhejiang Sci-Tech University,2020,43-44(自科六):840-845.
点击复制

铁皮石斛组培苗和栽培苗的circRNA检测及分析()
分享到：

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第43-44卷
期数:
2020年自科六期
页码:
840-845
栏目:
出版日期:
2020-11-27

文章信息/Info

Title:
Detection and analysis of circRNA in tissue cultured seedlinog and cultivated seedling of Dendrobium officinale
文章编号:
1673-3851 (2020) 11-0840-06
作者:
黄路瑶; 郁吉锋; 钱央央; 徐涛
浙江理工大学生命科学与医药学院，杭州 310018
Author(s):
HUANG Luyao; YU Jifeng; QIAN Yangyang; XU Tao
College of Life Sciences and Medicine, Zhejiang Sci-tech University, HangZhou 310018, China
关键词:
铁皮石斛; 组培苗; 栽培苗; circRNA; 差异性表达
分类号:
Q94-3
文献标志码:
A
摘要:
为了分析铁皮石斛组培苗与有菌根的栽培苗的circular RNA (circRNA)变化，采用高通量测序方法对铁皮石斛组培苗和栽培苗进行circRNA检测，并分析它们之间的差异。结果显示：两组4个样品共测出4115组不同的circRNA，其中有显著性差异的有27种，在实验组中17种为显著上调，10种为显著下调；GO富集分析一共检测出99组数据，其中显著性基因最多是核基因；KEGG富集分析表明，circRNA共有富集在7条KEGG通路上，其中丁酸甲酯代谢途径（Butanoate metabolism）和错配修复途径（Mismatch repair）具有显著性差异。以上结果可为进一步研究铁皮石斛形成菌根前后circRNA的变化提供参考。

参考文献/References:

［1］ Dong R, Ma X K, Chen L L, et al. Increased complexity of circRNA expression during species evolution［J］. RNA Biology, 2017, 14(8):1064-1074.
［2］ Liu Y Q, Zhang X, Liu M, et al. Direct detection of circRNA in real samples using reverse transcriptionrolling circle amplification［J］. Analytica Chimica Acta, 2020, 1101: 169-175.
［3］ George A K, Master K, Majumder A, et al. Circular RNAs constitute an inherent gene regulatory axis in the mammalian eye and brain［J］. Canadian Journal of Physiology And Pharmacology, 2019, 97(6):463-472.
［4］ Hu X L, Zhu M, Zhang X, et al. Identification and characterization of circular RNAs in the silkworm midgut following Bombyx mori cytoplasmic polyhedrosis virus infection［J］. RNA Biology, 2018, 15(2):292-301.
［5］ Guo R, Chen D F, Chen H Z, et al. Systematic investigation of circular RNAs in Ascosphaera apis, a fungal pathogen of honeybee larvae［J］. Gene, 2018, 678: 17-22.
［6］ He L B, Zhang A D, Xiong L, et al. Deep circular RNA sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection［J］. International Journal of Molecular Sciences, 2017, 18(9):1977-1993.
［7］ Zhang P J, Meng X W, Chen H J, el. PlantCircNet: A database for plant circRNAmiRNAmRNA regulatory networks［J］. DatabaseThe Journal of Biological Databases and Curation, 2017, 11: 1-8.
［8］ Huo L, Zhang P, Li C X, et al. Genomewide identification of circRNAs in pathogenic basidiomycetous yeast Cryptococcus neoformans suggests conserved circRNA host genes over kingdoms［J］. Genes, 2018, 9(3):118-131.
［9］ 刘致新, 孙文洲, 李佳欣, 等. circRNA的生物学功能及其在卵巢癌中的研究进展［J］. 癌症进展, 2020, 18(4):347-350.
［10］ 张晓云, 孔鹏, 窦永青, 等. circRNA肿瘤相关生物学功能的研究进展［J］. 生命的化学, 2020, 40(4): 607-613.

相似文献/References:

[1]俞婕,周小风,胡秀芳.铁皮石斛内生细菌的分离鉴定与酶活性分析[J].浙江理工大学学报,2013,30(06):896.
　YU Jie,ZHOU Xiao feng,HU Xiu fang.Separation and Identification of Endophytic Bacteria in Dendrobium Officinale and Analysis on Enzyme Activity[J].Journal of Zhejiang Sci-Tech University,2013,30(自科六):896.
[2]刘永红,杨渊华,梁宗锁.半夏组织培养及其生物碱代谢调控的研究[J].浙江理工大学学报,2016,35-36(自科5):759.
　LIU Yonghong,YANG Yuanhua,LIANG Zongsuo.Study on Tissue Culture of  Pinellia Ternata  and  Metabolism Regulation of Pinellia Alkaloids[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科六):759.
[3]管成林,王巧丽,胡秀芳.不同肥料对铁皮石斛生长、抗氧化酶活性及多糖积累的影响[J].浙江理工大学学报,2016,35-36(自科6):933.
　GUAN Chenglin,WANG Qiaoli,HU Xiufang.Effect of Fertilizers on Growth, Antioxidant Activity and Polysaccharide Accumulation of Dendrobium Officinale[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科六):933.
[4]李伯齐,郭建军,胡万哉,等.基于GA-BPNN优化铁皮石斛多糖提取工艺[J].浙江理工大学学报,2021,45-46(自科五):697.
　LI Boqi,GUO Jianjun,HU Wanzai,et al.Optimization of the extraction process of  Dendrobium officinale  polysaccharide based on GABPNN[J].Journal of Zhejiang Sci-Tech University,2021,45-46(自科六):697.
[5]钱央央,黄路瑶,范浩宇,等.铁皮石斛组培苗和栽培苗的lncRNA检测及其差异表达[J].浙江理工大学学报,2022,47-48(自科四):615.
　QIAN Yangyang,HUANG Luyao,FAN Haoyu,et al.Detection and differential expression of lncRNA in tissue cultured seedings and cultivated seedings of Dendrobium officinale[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科六):615.

备注/Memo

备注/Memo:
收稿日期：2020-06-28
网络出版日期：2020-09-03
基金项目：浙江省重点研发计划项目（2015C02030）
作者简介：黄路瑶(1995-)，女，江西宜春人，硕士研究生，主要从事天然药用植物资源开发方面的研究
通信作者：徐涛，E-mail：xutao1998cn@163.com
更新日期/Last Update: 2020-11-05