|本期目录/Table of Contents|

[1]高双玉,杜志游.关于二羧酸-三羧酸盐载体蛋白负调控黄瓜花叶病毒积累的初步研究[J].浙江理工大学学报,2018,39-40(自科5):613-618.
　GAO Shuangyu,DU Zhiyou.Preliminary study on the negative regulation of  Cucumber mosaic virus accumulation by dicarboxylatetricarboxylate carrier[J].Journal of Zhejiang Sci-Tech University,2018,39-40(自科5):613-618.
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关于二羧酸-三羧酸盐载体蛋白负调控黄瓜花叶病毒积累的初步研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第39-40卷
期数:
2018年自科5期
页码:
613-618
栏目:
出版日期:
2018-08-31

文章信息/Info

Title:
Preliminary study on the negative regulation of  Cucumber mosaic virus accumulation by dicarboxylatetricarboxylate carrier
文章编号:
1673-3851 (2018) 09-0613-06
作者:
高双玉; 杜志游
浙江理工大学生命科学学院，杭州 310018
Author(s):
GAO Shuangyu;  DU Zhiyou
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
黄瓜花叶病毒; 复制; 二羧酸三羧酸盐载体蛋白
分类号:
Q933
文献标志码:
A
摘要:
黄瓜花叶病毒(Cucumber mosaic virus，CMV)的复制场所位于液泡膜，但是参与调控CMV复制的寄主蛋白因子有待进一步研究。植物二羧酸三羧酸盐载体蛋白(Dicarboxylatetricarboxylate carrier，DTC)为线粒体膜和液泡膜定位的跨膜蛋白，参与二羧酸盐和三羧酸盐的跨膜转运。为了探索DTC在CMV复制中的调控作用，通过基于烟草脆裂病毒（ Tobacco rattle virus, TRV）的基因载体下调本氏烟体内DTC-2（NbDTC2）的mRNA水平，并通过GFP荧光和Western blot分析DTC2下调表达后对CMVΔ2beGFP积累量的影响，发现NbDTC2的下调表达增加CMV的积累水平；同时分析本氏烟DTC2的瞬时过表达对CMVΔCP eGFP积累的影响，通过观察eGFP荧光和Western blot检测，发现NbDTC2的过表达在一定程度上抑制病毒的积累。以上结果表明：DTC2负调控CMV的积累，可能参与植物对病毒的防御。

参考文献/References:

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［5］ Perret V, Florentz C, Dreher T, et al. Structural analogies between the 3’ tRNAlike structure of Brome mosaic virus RNA and yeast tRNATyr revealed by protection studies with yeast tyrosyltRNA synthetase［J］. European Journal of Biochemistry,1989,185(2):331-339.
［6］ Zhang X P, Liu D S, Yan T et al. Cucumber mosaic virus coat protein modulates the accumulation of 2b protein and antiviral silencing that causes symptom recovery in planta［J］. PLoS Pathogen,2017,13(7):e1006522.
［7］ Du Z Y, Chen A Z, Chen W H, et al. Nuclearcytoplasmic partitioning of Cucumber mosaic virus protein 2b determines the balance between its roles as a virulence determinant and an RNAsilencing suppressor［J］. Journal of Virology,2014,88(10),5228-5241.
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备注/Memo

备注/Memo:
收稿日期： 2018-05-11
网络出版日期： 2018-05-30
基金项目： 国家自然科学基金项目(31470007)
作者简介： 高双玉(1993- )，女，安徽阜阳，硕士研究生，主要从事植物病理方面的研究
通信作者： 杜志游，E-mail：duzy@zstu.edu.cn
更新日期/Last Update: 2018-09-12