|本期目录/Table of Contents|

[1]何欢,董文韬,郑娓诗,等.纳米羟基磷灰石颗粒负载p53基因对肝癌干细胞抑制效果[J].浙江理工大学学报,2017,37-38(自科2):270-276.
　HE Huan,DONG Wentao,ZHENG Weishi,et al.Inhibition Effect of p53Gene Loaded by Hydroxyapatite Nanoparticles on Hepatic Cancer Stem Cells[J].Journal of Zhejiang Sci-Tech University,2017,37-38(自科2):270-276.
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纳米羟基磷灰石颗粒负载p53基因对肝癌干细胞抑制效果()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第37-38卷
期数:
2017年自科2期
页码:
270-276
栏目:
出版日期:
2017-03-10

文章信息/Info

Title:
Inhibition Effect of p53Gene Loaded by Hydroxyapatite Nanoparticles on Hepatic Cancer Stem Cells
文章编号:
1673-3851 (2017) 02-0270-07
作者:
何欢; 董文韬; 郑娓诗; 孔祥东; 陈岑; 解纯刚
浙江理工大学生命科学学院，杭州 310018
Author(s):
HE Huan;  DONG Wentao;  ZHENG Weishi;  KONG Xiangdong;  CHEN Cen;  XIE Chungang
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
羟基磷灰石; 肿瘤干细胞; p 53  ; 抑制作用
分类号:
Q279
文献标志码:
A
摘要:
为评估纳米羟基磷灰石颗粒负载p53 基因对肝癌干细胞的抑制效果，采用化学沉淀法制备羟基磷灰石，对其进行表征，并检测其生物相容性。通过成球培养法获得肝癌干细胞，用经聚乙烯亚胺（PEI）修饰的纳米羟基磷灰石颗粒（HP）负载p53基因（HP p 53）处理肝癌干细胞，用MTT法、hoechst 33258染色综合评估杀伤效果。结果表明：所制备的纳米颗粒主要成分和晶型为羟基磷灰石，呈短棒状，长径为20～50 nm，短径约为20 nm，并具有良好的生物相容性和降解性；HP p53处理后的肝癌干细胞存活率显著下降；用Hoechst 33258染色观察到HP p53处理后的肝癌干细胞细胞核出现高亮光斑，细胞密度降低，表明所制备的纳米羟基磷灰石负载p53基因对肝癌干细胞具有显著的抑制效果。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2016-06-13
网络出版日期：2017-01-03
基金项目： 国家自然科学基金项目(50942023，51272236)
作者简介： 何〓欢(1990-)，女，湖南郴州人，硕士研究生，主要从事生物材料方面的研究
通信作者： 解纯刚，E-mail：chungxie@126.com
更新日期/Last Update: 2017-04-06