|本期目录/Table of Contents|

[1]刘端,吴俊,吴祥甫.人C 反应蛋白（CRP）基因的克隆及表达研究[J].浙江理工大学学报,2016,35-36(自科2):272-276.
　LIU Duan,WU Jun,WU Xiangfu.Cloning and Expression of C reactive Protein (CRP) Gene[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科2):272-276.
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人C 反应蛋白（CRP）基因的克隆及表达研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第35-36卷
期数:
2016年自科2期
页码:
272-276
栏目:
出版日期:
2016-03-10

文章信息/Info

Title:
Cloning and Expression of C reactive Protein (CRP) Gene
文章编号:
1673-3851 (2016) 02-0272-05
作者:
刘端; 吴俊; 吴祥甫
1.浙江理工大学生命科学学院，杭州310018；2．德赛诊断系统（上海）有限公司，上海201318
Author(s):
LIU Duan;  WU Jun;  WU Xiangfu
1. School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. DiaSys Diagnostic Systems (Shanghai) Co., Ltd., Shanghai 201318, China
关键词:
C 反应蛋白(CRP); 杆状病毒; 家蚕; Western Blot
分类号:
Q786
文献标志码:
A
摘要:
人C 反应蛋白（C reactive protein，CRP）参与人体炎症反应，是临床诊断中的重要检测指标。利用家蚕杆状病毒表达系统构建重组人 CRP 病毒vBmHis CRP ，接种家蚕五龄幼虫表达重组人CRP蛋白。利用自制抗CRP血清Western Blot检测重组蛋白得到目的条带，纯化后BCA法测定蛋白含量为30 μg/mL，质谱检测进一步确定该蛋白为目的蛋白。这为家蚕生物反应器规模化制备重组人CRP蛋白打下了基础，为解决临床CRP相关炎症反应快速诊断试剂盒中标准抗原的来源问题提供了依据。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2015-05-19
基金项目： 国家重大新药创制项目（2012ZX09102301-009）
作者简介： 刘端（1988-），男，河北大城人，硕士研究生，主要从事生物工程制药方面的研究
通信作者： 吴祥甫，E-mail:liuduansan@163.com
更新日期/Last Update: 2016-06-01