|本期目录/Table of Contents|

[1]沈梦薇,全丽丽,徐向群.有机溶剂对桦褐孔菌液体深层发酵多酚和多糖的产生与活性的影响[J].浙江理工大学学报,2016,35-36(自科1):93-98.
　SHEN Mengwei,QUAN Lili,XU Xiangqun.Effect of Organic Solvents on Production and Activity of Polyphenol and Polysaccharides from  Inonotus Obliquus  by Submerged Fermentation[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科1):93-98.
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有机溶剂对桦褐孔菌液体深层发酵多酚和多糖的产生与活性的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第35-36卷
期数:
2016年自科1期
页码:
93-98
栏目:
出版日期:
2016-01-10

文章信息/Info

Title:
Effect of Organic Solvents on Production and Activity of Polyphenol and Polysaccharides from  Inonotus Obliquus  by Submerged Fermentation
文章编号:
1673-3851 (2016) 01-0093-06
作者:
沈梦薇; 全丽丽; 徐向群
浙江理工大学理学院，杭州 310018
Author(s):
SHEN Mengwei;  QUAN Lili;  XU Xiangqun
School of Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
桦褐孔菌; 多酚; 有机溶剂; 多糖; 抗氧化活性
分类号:
Q936
文献标志码:
A
摘要:
在培养基中添加1g/L的不同有机溶剂（丙酮、甲醇、氯仿、甲苯和乙醇），讨论了有机溶剂对桦褐孔菌液体深层发酵多酚和多糖的产生的影响，并通过测定多酚的DPPH自由基清除率来探究有机溶剂对多酚抗氧化活性的影响。研究结果证明，添加有丙酮、甲苯与乙醇的培养基能显著提高桦褐孔菌生物量，比对照组提高2.68%、 2.27%和3.98%；以胞内外多酚含量和活性为指标，丙酮有机溶剂，胞内、胞外乙酸乙酯层和胞外正丁醇层多酚的产量分别为对照组的2.5、5.3和1.7倍，相对应的活性分别是对照组的1.3、1.3和3.0倍；以胞内外多糖含量为指标，有机溶剂均对胞外多糖有抑制作用，乙醇为最佳的添加剂使胞内多糖（在95 ℃、121 ℃下提取）的增加率达到9.67%和15.20%。推测可能是有机溶剂能有效地促进细胞膜的相容性，从而改变了细胞膜通透性，影响了细胞对培养基营养物质的吸收。同时，不同有机溶剂又会对不同种类次级代谢产物的合成产生不同的影响。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2015-01-13
基金项目： 浙江省科技厅公益技术项目(2012C20375)
作者简介： 沈梦薇(1990-)，女，浙江舟山人，硕士研究生，主要从事生物化学方面的研究
通信作者： 徐向群，E-mail：xuxiangqun@zstu.edu.cn
更新日期/Last Update: 2016-03-07