|本期目录/Table of Contents|

[1].崔磊, 黎江, 刘辉, 吴红平, 李林芳, 郝方元, 金华君, 钱其军[J].浙江理工大学学报,2015,33-34(自科4):558-564.
　CUI Lei,LI Jiang,LIU Hui,et al.Effect of SelfExpression of SIRPαFc Fusion Protein on T Cell Function[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科4):558-564.
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崔磊, 黎江, 刘辉, 吴红平, 李林芳, 郝方元, 金华君, 钱其军()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科4期
页码:
558-564
栏目:
出版日期:
2015-07-10

文章信息/Info

Title:
Effect of SelfExpression of SIRPαFc Fusion Protein on T Cell Function
文章编号:
1673-3851 (2015) 05-0712-06
作者:
1. 浙江理工大学生命科学院新元医学与生物技术研究所, 杭州 310018;2. 第二军医大学上海东方肝胆外科医院病毒基因治疗实验室, 上海 200438
Author(s):
CUI Lei;  LI Jiang;  LIU Hui;  WU Hongping;  LI Linfang;  HAO Fangyuan;  JIN Huajun;  QIAN Qijun
1. School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China;2. Eastern Hepatobiliary Surgical Hospital, the Second Military Medical University, Shanghai 200438, China
关键词:
CD47;  T细胞;  腺病毒;  肝癌
分类号:
Q789
文献标志码:
A
摘要:
探讨携带SIRPαFc(SF)融合基因蛋白基因的重组腺病毒Ad35SF感染T细胞后对T细胞功能的影响。腺病毒Ad35 SF感染Jurkat T细胞后,通过Western blotting及ELISA方法检测细胞上清中SF融合蛋白的大小与表达量;通过流式分析技术检测细胞表面CD47的封闭情况;通过CCK8方法检测细胞的增殖与对肿瘤细胞的杀伤作用。结果表明,Ad35 SF构建成功,其感染Jurkat T细胞后,能在其内表达并分泌产生大小约为48 kD的SF蛋白,细胞上清中SF的表达量可到达19.1 μg/mL。Ad35 SF(MOI =1)感染Jurkat细胞18 h后,细胞CD47阳性比例从97.06％下降到15.32％;当Ad35 SF感染复数增加至5,10时,CD47阳性比率进一步降低至2.94%和1-73%。相对于空病毒对照组,Ad35 SF感染Jurkat细胞48 h后其增殖效果提高了29％,对肝癌细胞株Hep 3B的杀伤作用提升25.6％( p <0.01)。结果表明构建的重组腺病毒Ad35 SF能有效感染Jurkat细胞并在其内表达分泌SF蛋白,封闭细胞表面的CD47,同时显著提高Jurkat细胞的增殖能力和对肝癌细胞的杀伤作用。

参考文献/References:

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相似文献/References:

[1]崔磊,黎江,刘辉,等.自表达SIRPα Fc融合蛋白对T细胞功能的影响[J].浙江理工大学学报,2015,33-34(自科5):712.
　CUI Lei,LI Jiang,LIU Hui,et al.Effect of SelfExpression of SIRPαFc Fusion Protein on T Cell Function[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科4):712.

备注/Memo

备注/Memo:
收稿日期： 2014-12-15
基金项目： 国家自然科学基金项目(81071850)
作者简介： 崔磊(1989-),男,山西运城人,硕士研究生,主要从事靶向基因—病毒治疗方面的研究
通信作者： 钱其军,E-mail:qianqj@sino-gene.com
更新日期/Last Update: 2015-09-24