|本期目录/Table of Contents|

[1]李杰,谭淑敏,王小飞,等.家蚕表达人促红细胞生成素口服急性毒性和初步药效学研究[J].浙江理工大学学报,2014,31-32(自科6):728-733.
　LI Jie,TAN Shu min,WANG Xiao fei,et al.Research on Acute Oral Toxicity and Preliminary Pharmacodynamics ofHuman Erythropoietin Expressed in Bombyx Mori[J].Journal of Zhejiang Sci-Tech University,2014,31-32(自科6):728-733.
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家蚕表达人促红细胞生成素口服急性毒性和初步药效学研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第31-32卷
期数:
2014年自科6期
页码:
728-733
栏目:
(自科)生物与生命科学
出版日期:
2014-11-10

文章信息/Info

Title:
Research on Acute Oral Toxicity and Preliminary Pharmacodynamics ofHuman Erythropoietin Expressed in Bombyx Mori
文章编号:
1673-3851 (2014) 06-0728-06
作者:
李杰;  谭淑敏;  王小飞;  舒特俊;  于威;  张耀洲;  陈剑清
1. 浙江理工大学, a. 生物化学研究所; b. 浙江省家蚕生物反应器和生物医药重点实验室, 杭州 310018;2. 天津国际生物医药联合研究院, 天津 300457; 3. 天津大学药物科学与技术学院,天津 300457
Author(s):
LI Jie;  TAN Shumin;  WANG Xiaofei;  SHU Tejun;  YU Wei; ZHANG Yaozhou;  CHEN Jianqing
1a. Institute of Biochemistry; 1b. Zhejiang Provincial Key Laboratory of Silkworm Bioreactorand Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China;2.Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China;3. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300192, China
关键词:
家蚕;  EPO;  口服;  急性毒性;  肾性贫血
分类号:
Q816
文献标志码:
A
摘要:
为了研究家蚕表达的重组人促红细胞生成素(BmrhEPO)口服治疗肾性贫血的疗效并评价安全性,进行了小鼠口服BmrhEPO急性毒性实验,结果显示其对小鼠基本无毒或毒性极低,小鼠口服BmrhEPO最大耐受剂量大于147 882 IU/kg。肾性贫血小鼠以剂量为62 865 IU/kg和20 955 IU/kg的两个剂量组连续口服BmrhEPO一周,以小鼠血液网织红细胞数及百分率为药效指标,结果显示口服有药效,并且药效与剂量呈正相关。本实验为开发重组人促红细胞生成素口服药物提供了理论指导和实验基础。

参考文献/References:

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［7］ Quelle D E, Lynch K J, BurkertSmith R E, et al. Phosphorylatable and epitopetagged human erythropoietins: utility and purification of native baculovirusderived forms［J］. Protein Expr Purif, 1992, 3(6): 461-469.
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备注/Memo

备注/Memo:
收稿日期： 2014-01-14
基金项目： 国家高技术研究发展计划“863”项目(2011AA100603)
作者简介： 李杰(1988-),男,湖北谷城人,硕士研究生,研究方向为生物反应器与蛋白质组学
通信作者： 陈剑清,Email:cjqgqj@126.com
更新日期/Last Update: 2014-11-24