|本期目录/Table of Contents|

[1]马国兴,马登旭,杨力媛,等.水稻单个端粒长度定量测定及分析[J].浙江理工大学学报,2013,30(04):585-589.
　MA Guixing,MA Dengxu,YANG Liyuan,et al.Analysis and Quantitative Determination of Single Telomere Length in Rice[J].Journal of Zhejiang Sci-Tech University,2013,30(04):585-589.
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水稻单个端粒长度定量测定及分析()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年04期
页码:
585-589
栏目:
(自科)生物与生命科学
出版日期:
2013-07-10

文章信息/Info

Title:
Analysis and Quantitative Determination of Single Telomere Length in Rice
文章编号:
1673-3851 (2013) 04-0585-05
作者:
马国兴;  马登旭;  杨力媛;  郑洁;  韦敬航;  刘小川
浙江理工大学生物工程研究所, 杭州 310018
Author(s):
MA Guixing;  MA Dengxu;  YANG Liyuan;  ZHENG Jie;  WEI Jinghang;  LIU Xiaochuan
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
水稻;  单个端粒;  PCR技术;  定量测定;  端粒长度
分类号:
Q291
文献标志码:
A
摘要:
端粒是真核生物线性染色体末端的特殊结构,一定长度的端粒在维持真核生物遗传信息完整性和染色体结构稳定性中起重要作用。建立一种简单快捷定量测定水稻单个端粒绝对长度的方法对研究水稻各个染色体端粒具有积极的意义。研究将特殊接头连接到水稻染色体端粒的Goverhang末端,并利用近端粒区域的特定引物以及下游接头引物,通过PCR技术,获得所需鉴定的端粒,结合DNA凝胶电泳成像分析系统,定量检测水稻单个端粒的长度。结果显示本方法可以通过少量水稻基因组DNA有效测定单个端的粒长度。

参考文献/References:

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相似文献/References:

[1]马登旭,杨力媛,马国兴,等.水稻端粒酶RNA候选序列的克隆及特征解析[J].浙江理工大学学报,2013,30(04):579.
　MA Dengxu,YANG Liyuan,MA Guoxing,et al.Cloning and Characteristic Analysis of Rice Telomerase RNA Candidate Sequence[J].Journal of Zhejiang Sci-Tech University,2013,30(04):579.
[2]杨力媛,郑洁,马登旭,等.水稻端粒酶RNA模板基因序列鉴定体系的建立[J].浙江理工大学学报,2013,30(04):590.
　YANG Liyuan,ZHENG Jie,MA Dengxu,et al.Establishment of Identification System of TelomeraseRNA Template Gene Sequence in Rice[J].Journal of Zhejiang Sci-Tech University,2013,30(04):590.
[3]朱莲莲,林高强,叶聪莹,等.水稻端粒酶RNA候选序列的鉴定[J].浙江理工大学学报,2016,35-36(自科1):145.
　ZHU Lianlian,LIN Gaoqiang,YE Congying,et al.Identification of Telomerase RNA Candidate  Sequence in Rice ( Oryza Sativa L .)[J].Journal of Zhejiang Sci-Tech University,2016,35-36(04):145.
[4]叶聪莹,黄倩,马国兴,等.水稻端粒长度测定及其遗传特性研究[J].浙江理工大学学报,2016,35-36(自科3):463.
　YE Congying,HUANG Qian,MA Guoxing,et al.Measurement of Telomeric Length and Hereditary Character of Oryza Sativa[J].Journal of Zhejiang Sci-Tech University,2016,35-36(04):463.

备注/Memo

备注/Memo:
收稿日期： 2012-10-10
作者简介： 马国兴(1987-),男,河北唐山市人,硕士研究生,研究方向为植物端粒及分子遗传研究。
通信作者： 刘小川,电子信箱:xcliu@zstu.edu.cn
更新日期/Last Update: 2013-10-24