|本期目录/Table of Contents|

[1]马登旭,杨力媛,马国兴,等.水稻端粒酶RNA候选序列的克隆及特征解析[J].浙江理工大学学报,2013,30(04):579-584.
　MA Dengxu,YANG Liyuan,MA Guoxing,et al.Cloning and Characteristic Analysis of Rice Telomerase RNA Candidate Sequence[J].Journal of Zhejiang Sci-Tech University,2013,30(04):579-584.
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水稻端粒酶RNA候选序列的克隆及特征解析()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年04期
页码:
579-584
栏目:
(自科)生物与生命科学
出版日期:
2013-07-10

文章信息/Info

Title:
Cloning and Characteristic Analysis of Rice Telomerase RNA Candidate Sequence
文章编号:
1673-3851 (2013) 04-0579-06
作者:
马登旭;  杨力媛;  马国兴;  郑洁;  应畅;  刘小川
浙江理工大学生命科学学院生物工程研究所, 杭州 310018
Author(s):
MA Dengxu;  YANG Liyuan;  MA Guoxing;  ZHENG Jie;  YING Chang;  LIU Xiaochuan
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
水稻;  PEG6000;  端粒酶RNA;  特征解析
分类号:
Q781
文献标志码:
A
摘要:
端粒酶是由端粒酶反转录酶和端粒酶RNA构成,端粒酶RNA中含有端粒合成所需的模板序列。现仅获得了拟南芥的端粒酶RNA序列。为了建立快速有效的富集植物端粒酶的方法并克隆水稻的端粒酶RNA序列，研究中使用不同浓度的PEG6000选择性的沉淀端粒酶萃取液中的杂蛋白。结果表明当PEG6000浓度为4%时,端粒酶活力最高,并可以有效降低核糖体28S rRNA和18S rRNA的干扰。从端粒酶蛋白液中提取RNA,并在其3′端连接接头,在模板区设计上游引物,PCR扩增获得一条292 bp的未知片段,其中含有端粒模板序列5′AAACCCTAA3′。将该片段及拟南芥端粒酶RNA的转录调控区进行序列比对分析发现,两者具有类似的转录调控元件。因此,初步判定292bp的目的片段为水稻端粒酶RNA的模板下游序列。

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2012-11-07
作者简介： 马登旭(1985-),男,山东济宁人,硕士研究生,主要从事端粒及端粒酶的分子遗传研究。
通信作者： 刘小川,电子信箱:xcliu@zstu.edu.cn
更新日期/Last Update: 2013-10-24