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[1]肖睿娟,张越峰,潘素晶,等. 溶瘤腺病毒ZD55MnSOD联合TPL对骨髓瘤细胞的生长抑制效应[J].浙江理工大学学报,2012,29(03):408-413.
　XIAO Rui juan,ZHANG Yue feng,PAN Su jing,et al. The Growth Inhibiting Effect to Multiple Myeloma Cells by OncolyticAdenovirus ZD55MnSOD Combined with TPL[J].Journal of Zhejiang Sci-Tech University,2012,29(03):408-413.
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 溶瘤腺病毒ZD55MnSOD联合TPL对骨髓瘤细胞的生长抑制效应()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年03期
页码:
408-413
栏目:
(自科)生物与生命科学
出版日期:
2012-06-28

文章信息/Info

Title:
 The Growth Inhibiting Effect to Multiple Myeloma Cells by OncolyticAdenovirus ZD55MnSOD Combined with TPL
文章编号:
16733851 (2012) 03040806
作者:
 肖睿娟;  张越峰;  潘素晶;  王毅刚
 浙江理工大学生命科学学院新元医学与生物技术研究所, 杭州 310018
Author(s):
 XIAO Ruijuan;  ZHANG Yuefeng;  PAN Sujing;  WANG Yigang
 Xinyuan Institute of Medicine and Biotechnology, Zhejiang SciTech University,Hangzhou 310018, China
关键词:
 ; ZD55MnSOD;  TPL;  骨髓瘤细胞;  抑制效应
分类号:
Q789
文献标志码:
A
摘要:
    研究利用携带MnSOD的溶瘤腺病毒AdE1AΔE1B55KdMnSOD (ZD55MnSOD)联合小分子化合物雷公藤内酯醇（Triptolide,TPL）对骨髓瘤细胞株RPMI8226、U266生长的体外抑制效应，希望找到解决化疗药物在临床使用中所出现的疗效低及毒副作用大的方法。采用CCK8比色分析方法表明100 MOI ZD55MnSOD与TPL（2 ng/mL、4 ng/mL）联合处理后，〖JP2〗RPMI 8226及U266的细胞存活率均显著低于单用ZD55MnSOD或TPL（P<0.05）；用结晶紫染色法定性分析了ZD55MnSOD联合TPL对两种骨髓瘤细胞的杀伤作用；最后Hoechst33342染色法观察细胞凋亡也显示联合应用ZD55MnSOD与TPL处理组的细胞凋亡现象比二者单独使用均要显著。本研究初步证实ZD55MnSOD与TPL联合具有协同作用，能有效地在体外抑制骨髓瘤细胞RPMI 8226和U 266的生长。

参考文献/References:

［1］Schwartz R N, Vozniak M. Current and emerging treatments for multiple myeloma［J］. J Manag Care Pharm, 2008, 14(7): 1218.
［2］ 王恒邦, 许建华, 温彩霞, 等. 雷公藤内酯醇体内外抗肿瘤作用［J］. 福建医科大学学报, 2007, 41(4): 304307.〖JP〗
［3］ Kinnula V L, Crapo J D. Superoxide dismutase in the lung and human lung diseases ［J］. Am J Rispir Crit Care Med, 2001, 67(12): 16001609.
［4］ Hur G C, Cho S J, Kim C H, et al. Manganese superoxide dismutase expression correlates with chemosensitivity in human gastric cancer cell lines ［J］. Clin Cancer Res, 2003, 15(4): 57685775.
［5］ Liu X Y. A new anticancer strategy—GeneVirotherapy Therapy of cancer［J］. Chinese J Cancer Biother, 2001, 8(1): 1.
［6］ Zhang Z L, Zou W G, Luo C X, et al. An armed oncolytic adenovirus system, ZD55gene, demonstrating potent antitumoral efficacy［J］. Cell Res , 2003, 13(6): 481489.
［7］ Rampling R, Cruickshank G, Papanastassiou V, et al. Toxicity evaluation of replication competent herpes simplex virus(ICP 34.5 null mutant 1716) in patients with recurrent malignant glioma［J］. Gene Ther, 2000, 7(10): 859866.
［8］ Markert J M, Medlock M D, Rabkin S D, et al. Conditionally replicating herpes simplex virus mutant, G207 for the treatment of malignant glioma: results of a phase I trial［J］. Gene Ther, 2000, 7(10): 867874.
［9］ Mentel R, Dopping G, Wegner U, et al. Adenovirusreceptor interaction with human lymphocytes［J］. J Med Virol, 1997, 51(3): 252257.
［10］Rebel V I, Hartnett S, Denham J, et al. Maturation and lineagespecific expression of the coxsackie and adenovirus receptor in hematopoietic cells［J］. Stem cells, 2000, 18(3): 176182.
［11］ Douglas J T, Kim M, Sumerel L A, et al. Efficient oncolysis by a replicating adenovirus (ad) in vivo is critically dependent on tumor expression of primary ad receptors［J］. Cancer Res , 2001, 61(3): 813817.
［12］ Wickham T J, Mathias P, Cheresh D A, et al. Interins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachment［J］. Cell, 1993, 73(2): 309319.

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备注/Memo

备注/Memo:
 收稿日期： 2011-09-20
基金项目： 国家自然科学基金资助项目(30800093);浙江省自然科学基金资助项目(Y2090935);浙江理工大学科研启动基金（1016845Y,1016834Y）
作者简介： 肖睿娟(1988-)，女，河南周口人，硕士研究生，主要从事肿瘤的靶向基因病毒治疗的研究。
通讯作者： 王毅刚，电子邮箱：wangyigang43@163.com
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