|本期目录/Table of Contents|

[1]雷文,王世兵,马步云,等.AFP与CEA基因的启动子克隆及肿瘤细胞特异性表达的研究[J].浙江理工大学学报,2012,29(01):119-124.
　LEI Wen,WANG Shi\|bing,MA Bu\|yun,et al. Cloning of AFP and CEA Gene Promoters and Analysis ofTheir Tumor Specific Activity[J].Journal of Zhejiang Sci-Tech University,2012,29(01):119-124.
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AFP与CEA基因的启动子克隆及肿瘤细胞特异性表达的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年01期
页码:
119-124
栏目:
(自科)生物与生命科学
出版日期:
2012-02-28

文章信息/Info

Title:
 Cloning of AFP and CEA Gene Promoters and Analysis ofTheir Tumor Specific Activity
文章编号:
16733851 (2012) 01011906
作者:
 雷文;  王世兵;  马步云;  叶静;  夏玉龙;  王毅刚
 浙江理工大学生命科学学院新元医学与生物技术研究所， 杭州 310018
Author(s):
 LEI Wen;  WANG Shi\|bing;  MA Bu\|yun;  YE Jing;  XIA Yu\|long;  WANG Yi\|gang
 Xin yuan Institute of Medicine and Biochnology, School of Life Sciences,Zhejiang Sci\|Tech University, Hangzhou 310018, China
关键词:
 ; AFP;  CEA;  双荧光素酶报告基因;  转录靶向性
分类号:
Q789
文献标志码:
A
摘要:
    为了研究AFP和CEA基因启动子的转录活性与肿瘤特异性，本实验利用PCR 技术扩增AFP和CEA基因上游序列，并将其克隆到 pGL3\|Basic载体中构建pGL3\|AFP和pGL3\|CEA 荧光报告质粒。将构建的pGL3\|AFP和pGL3\|CEA质粒分别与pRL\|TK共转染人结肠癌细胞株SW620、肝癌细胞株Huh\|7、 肺癌细胞株A549、 宫颈癌细胞株Hela、 人肝正常细胞株QSG\|7701、 人肺正常细胞株Beas\|2b，用双荧光报告系统检测这两种质粒在不同细胞里的荧光活性表达。 酶切和测序结果证实成功构建了双荧光素酶报告质粒pGL3\|AFP和pGL3\|CEA，双荧光素酶报告基因检测结果证明AFP和CEA均具有一定的肿瘤特异性。这些结果表明AFP 和CEA 均具有一定的肿瘤特异性，为进一步研究AFP和CEA基因的表达调控机制和探讨靶向基因\|病毒治疗提供依据。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2011-04-27
基金项目： 国家自然科学基金资助项目(30800093)；浙江省自然科学基金资助项目(Y2090935)；浙江理工大学科研启动基金（1016845\|Y,1016834\|Y）;浙江省大学生科技创新计划资助课题（2010R406036）
作者简介： 雷文（1986-），女，安徽宣城人，硕士研究生，主要从事肿瘤的靶向基因\|病毒治疗的研究。
通讯作者： 王毅刚，电子邮箱：wangyigang43@163.com
更新日期/Last Update: