|本期目录/Table of Contents|

[1]刘轩,胡秀俊,周严,等.丝素蛋白溶液二级结构协同稳定性调控研究[J].浙江理工大学学报,2026,55-56(自科五):660-669.
　LIUXuan,HUXiujun,ZHOUYan,et al.A study on the synergistic regulation of secondary structure and stability of silk fibroin solutions[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科五):660-669.
点击复制

丝素蛋白溶液二级结构协同稳定性调控研究()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第五期
页码:
660-669
栏目:
出版日期:
2026-09-20

文章信息/Info

Title:
A study on the synergistic regulation of secondary structure and stability of silk fibroin solutions
文章编号:
1673-3851(2026) 09-0660-10
作者:
刘轩; 胡秀俊; 周严;  肖林蓉; 江国华
浙江理工大学 ,a. 材料科学与工程学院;b. 全省丝绸与蚕丝蛋白新材料重点实验室 ,杭州 310018
Author(s):
LIUXuan ; HUXiujun ; ZHOUYan ; XIAOLinrong; JIANGGuohua
a. School of Materials Science& Engineering; b. Zhejiang Provincial Key Laboratory of Silk and Silk Protein Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
丝素蛋白溶液 ; 稳定性调控; 烟酰胺; 聚酰胺-胺; β-折叠 ;  高温灭菌; 二级结构
分类号:
TS102.33
文献标志码:
A
摘要:
针对丝素蛋白(Silk fibroin, SF)溶液在储存过程中易发生分子聚集和凝胶化的 问题 ,探究烟酰胺(Nicotinamide, NAM)与聚酰胺-胺(Polyamidoaminedendrimer, PAMAM)作为稳定剂的协同作用机制 ,评估不同稳定剂组合在 未 灭 菌 及 灭 菌 条 件 下 对 SF 溶 液 稳 定 性 的 影 响 。 实 验 结 果 表 明 , 配 方 (1% SF-3% NAM-0.5% PAMAM )经高温灭菌后 ,21d 内吸光度低于 0.318,粒径后期日增长率仅为 1.04% ,α-螺旋与无规卷曲总含量保留率达91.39% ,β-折叠转化率较 SF组降低21.01% ,该复合稳定剂显著提升了SF溶液在高温灭菌及后续长期储存的稳定性 。机制研究表明 ,NAM与 PAMAM通过竞争性形成氢键及空间位阻效应双机制协同作用 ,分别从分子内外实现构象调控与聚集阻隔 ,在保障性能的同时具备良好的工艺兼容性与成本可控性 。研究结果为丝素蛋白液体敷料的稳定化应用提供了可行方案。

参考文献/References:

[1] Liu B, Song Y W, Jin L, et al. Silk structure and degradation [J]. Colloids and Surfaces B: Biointerfaces, 2015, 131: 122-128.
[2] Vepari C, Kaplan D L. Silk as a biomaterial [J]. Progress in Polymer Science, 2007, 32 (8/9): 991-1007.
[3] Floren M, Migliaresi C, Motta A. Processing techniques and applications of silk hydrogels in bioengineering [J]. Journal of Functional Biomaterials, 2016, 7 (3): 26.
[4] Sun W, Incitti T, Migliaresi C, et al. Viability and neuronal differentiation of neural stem cells encapsulated in silk fibroin hydrogel functionalized with an IKVAV peptide [J]. Journal of Tissue Engineering and Regenerative Medicine, 2017, 11 (5): 1532-1541.
[5] Smeets R, Tauer N, Vollkommer T, et al. Tissue adhesives in reconstructive and aesthetic surgery: application of silk fibroin-based biomaterials [J]. International Journal of Molecular Sciences, 2022, 23 (14): 7687.
[6] Partlow B P, Tabatabai A P, Leisk G G, et al. Silk fibroin degradation related to rheological and mechanical properties [J]. Macromolecular Bioscience, 2016, 16 (5): 666-675.
[7] Chen S Y, Liu M Y, Huang H M, et al. Mechanical properties of Bombyx mori silkworm silk fibre and its corresponding silk fibroin filament: a comparative study [J]. Materials & Design, 2019, 181: 108077.
[8] Li J, Yang F, Liu D Y, et al. Graphene composite paper synergized with micro/nanocellulose-fiber and silk fibroin for flexible strain sensor [J]. International Journal of Biological Macromolecules, 2023, 240: 124439.
[9] Lu S J, Tang X C, Lu Q Q, et al. In vitro and in vivo degradation of silk fibers degummed with various sodium carbonate concentrations [J]. Materials Today Communications, 2021, 27: 102369.
[10] 张野妹，潘志娟。再生天然丝蛋白的分子构象转化特征与机理的研究现状 [J]. 丝绸，2013, 50 (7): 34-40,46.
[11] Li X G, Wu L Y, Huang M R, et al. Conformational transition and liquid crystalline state of regenerated silk fibroin in water [J]. Biopolymers, 2008, 89 (6): 497-505.
[12] Huang W, Ling S, Li C, et al. Silkworm silk-based materials and devices generated using bio-nanotechnology [J]. Chemical Society Reviews, 2018, 47 (17): 6486-6504.
[13] Khan S N, Khan S, Misba L, et al. Synergistic fungicidal activity with low doses of eugenol and amphotericin B against Candida albicans [J]. Biochemical and Biophysical Research Communications, 2019, 518 (3): 459-464.
[14] Abedi-Gaballu F, Dehghan G, Ghaffari M, et al. PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy [J]. Applied Materials Today, 2018, 12: 177-190.
[15] Domański D M, Klajnert B, Bryszewska M. Influence of PAMAM dendrimers on human red blood cells [J]. Bioelectrochemistry, 2004, 63 (1/2): 189-191.
[16] Heiden T C K, Dengler E, Kao W J, et al. Developmental toxicity of low generation PAMAM dendrimers in zebrafish [J]. Toxicology and Applied Pharmacology, 2007, 225 (1): 70-79.
[17] Liu J, Huang R, Li G, et al. Generation of nano-pores in silk fibroin films using silk nanoparticles for full-thickness wound healing [J]. Biomacromolecules, 2021, 22 (2): 546-556.
[18] Manning M C, Chou D K, Murphy B M, et al. Stability of Protein Pharmaceuticals: An Update [J]. Pharmaceutical Research, 2010, 27 (4): 544-575.
[19] Zhang X Y, Xiao L Y, Ding Z Z, et al. Fragile-tough mechanical reversion of silk materials via tuning supramolecular assembly [J]. ACS Biomaterials Science & Engineering, 2021, 7 (6): 2337-2345.
[20] Bissett D L, Miyamoto K, Sun P, et al. Topical niacinamide reduces yellowing, wrinkling, red blotchiness, and hyperpigmented spots in aging facial skin [J]. International Journal of Cosmetic Science, 2004, 26 (5): 231-238.
[21] Marques C, Hadjab F, Porcello A, et al. Mechanistic insights into the multiple functions of niacinamide: therapeutic implications and cosmeceutical applications in functional skincare products [J]. Antioxidants, 2024, 13 (4): 425.
[22] Zhou M, Lin F, Li W D, et al. Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment [J]. International Journal of Biological Macromolecules, 2021, 166: 1335-1351.
[23] Yan C J, Zhou Z. Walnut pellicle phenolics greatly influence the extraction and structural properties of walnut protein isolates [J]. Food Research International, 2021, 141: 110163.
[24] 李炜祥，凌静，王详，等。单宁酸对丝素蛋白二级结构的影响作用研究 [J]. 丝绸，2023, 60 (6): 1-6.
[25] Barth A. Infrared spectroscopy of proteins [J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2007, 1767 (9): 1073-1101.

备注/Memo

备注/Memo:
收稿日期 : 2025-12-23 网络出版日期 : 2026-05-26作者简介 : 刘 轩 (2001— ) ,女 ,湖南衡阳人 ,硕士研究生 ,主要从事生物大分子组装与功能材料的研究。通信作者: 江国华,E-mail:ghjiang_cn@zstu. edu. cnresearch findings provide a feasible solution for the stabilization application of silk fibroin-based liquid dressings.Key words: silk fibroin solution; stability regulation; nicotinamide; polyamidoamine dendrimer; β-sheet; high-temperaturesterilization; secondary structure
更新日期/Last Update: 2026-09-21