|本期目录/Table of Contents|

[1]任珂瑶,刘舒欣,徐海舟,等.白腐真菌BZ103的鉴定及产漆酶培养条件优化[J].浙江理工大学学报,2026,55-56(自科三):352-362.
 REN Keyao,LIUShuxin,XU Haizhou,et al.Identification of a white-rot fungus BZ103and optimization of laccase production medium[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科三):352-362.
点击复制

白腐真菌BZ103的鉴定及产漆酶培养条件优化()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第三期
页码:
352-362
栏目:
出版日期:
2026-05-10

文章信息/Info

Title:
Identification of a white-rot fungus BZ103and optimization of laccase production medium
文章编号:
1673-3851(2026) 05-0352-
作者:
任珂瑶 刘舒欣 徐海舟 李丽 张殿朋 赵洪新
1. 浙江理工大学生命科学与医药学院 ,杭州 310018;2. 温州市乡村振兴发展中心 ,温州 325000;3. 北京市农林科学院植物保护环境保护研究所 ,北京 100097
Author(s):
REN Keyao LIUShuxin XU Haizhou LI Li ZHANG Dianpeng ZHAO Hongxin
1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China;2. Wenzhou Rural Revitalization and Development Center, Wenzhou 325000, China;3. Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing100097, China
关键词:
漆酶 白腐真菌 分子鉴定 培养基优化响应面法
分类号:
Q939.9
文献标志码:
A
摘要:
白腐真菌代谢过程中产生的漆酶(EC 1.10.3.2)能够降解染料、酚类、木质素等大分子物质 ,挖掘高酶活性的白腐真菌菌株 ,对清除环境残留大分子污染物及防止环境污染具有重要意义 。 以一株分离自温州市郊白腐真菌 BZ103为材料 ,通过ITS序列与 18SrDNA序列系统发育分析确定其分类归属 ,并采用单因素实验与响面法优化其产漆酶培养条件 。结果表明 :菌株 BZ103与黄孢原毛平革菌(Phanerochaetechrysosporium)亲缘关系近 ,为黄孢原毛平革菌的一株新菌株 ;初始 pH值、碳源、氮源、Mn2+ 浓度、Cu2+ 浓度、碳氮比及接种量对漆酶产量具有显著影响 , 当初始 pH值在 3.5~4.5,碳源为葡萄糖、氮源为蛋白胨 ,Mn2+ 浓度为 0.6~ 1.2 mmol/L、Cu2+ 浓在 0.1~0.2 mmol/L、碳氮比在 60~120、接种量体积分数为 6%~ 10%时均可提高漆酶产量 ; 经 Box-Behnken设的响应面法优化 ,最优产酶培养基配方为葡萄糖 20g/L、蛋白胨5g/L、初始 pH值4.0、Mn2+ 浓度 0.54mmol/L、Cu2浓度 0.11mmol/L、碳氮比91.6和接种量体积分数8.4% ,在该条件下漆酶活性最高达到 644.3U/L,较优化前提14.98倍 。该研究可为漆酶的高效生产与环境污染的生物修复提供优良菌种资源 ,也为相关微生物的发酵工艺优与应用开发提供了重要的理论依据。

参考文献/References:

[1] 何兴舟. 环境与疾病:环境病因学研究中值得注意的问题[J]. 中华流行病学杂志, 2003, 24(10): 857-859.
[2] Ugrina M, Jurić A. Current trends and future perspectives in the remediation of polluted water, soil and air: A review[J]. Processes, 2023, 11(12): 3270.
[3] 季秀玲, 魏云林. 低温微生物环境污染修复技术研究进展[J]. 环境污染治理技术与设备, 2006, 7(10): 6-11.
[4] Torres-Farradó G, Thijs S, Rineau F, et al. White rot fungi as tools for the bioremediation of xenobiotics: A review[J]. Journal of Fungi, 2024, 10(3): 167.
[5] Latif W, Ciniglia C, Iovinella M, et al. Role of white rot fungi in industrial wastewater treatment: A review[J]. Applied Sciences, 2023, 13(14): 8318.
[6] Biko O D V, Viljoen-Bloom M, Van W H. Microbial lignin peroxidases: Applications, production challenges and future perspectives[J]. Enzyme and Microbial Technology, 2020, 141: 109669.
[7] Zhang Y, Chen X X, Fang L X, et al. Fenton-reaction-aid selective delignification of lignocellulose by Inonotus obliquus to improve enzymatic saccharification[J]. Fuel, 2023, 333(1): 126355.
[8] Chen J, Hong K, Ma L, et al. Effect of time series on the degradation of lignin by Trametes gibbosa: products and pathways[J]. International Journal of Biological Macromolecules, 2024, 281(1): 136236.
[9] Arfin I, Suman S K, Vempatapu B P, et al. Pyrene remediation by Trametes maxima: an insight into secretome response and degradation pathway[J]. Environmental Science and Pollution Research, 2022, 29(29): 44135-44147.
[10] 殷茹. 白腐真菌对双酚F的降解机制及应用研究[D]. 广州: 广州大学, 2021: 5-11.
[11] Patel A K, Singhania R R, Albarico F P J B, et al. Organic wastes bioremediation and its changing prospects[J]. Science of the Total Environment, 2022, 824: 153889.
[12] Kumar A, Kumar A P. Biotechnological applications of manganese peroxidases for sustainable management[J]. Frontiers in Environmental Science, 2022, 10: 875157.
[13] Li Z X, Wang X, Ni Z J, et al. In-situ remediation of carbofuran-contaminated soil by immobilized white-rot fungi[J]. Polish Journal of Environmental Studies, 2020, 29(2): 1237-1243.
[14] Asemoloye M D, Marchisio M A, Gupta V K, et al. Genome-based engineering of ligninolytic enzymes in fungi[J]. Microbial Cell Factories, 2021, 20(1): 20.
[15] Serbent M P, Gonçalves Timm T, Vieira Helm C, et al. Growth, laccase activity and role in 2,4-D degradation of Lentinus crinitus (L.) Fr. in a liquid medium[J]. Biocatalysis and Agricultural Biotechnology, 2023, 50: 102682.
[16] 兴勤鑫, 包月瑶, 彭静, 等. 漆酶在环境保护中的应用研究进展[J]. 环境保护与循环经济, 2024, 44(4): 17-21.
[17] Zhou W, Zhang W, Cai Y. Laccase immobilization for water purification: a comprehensive review[J]. Chemical Engineering Journal, 2021, 403: 126272.
[18] Schuetz M, Benske A, Smith R A, et al. Laccases direct lignification in the discrete secondary cell wall domains of protoxylem[J]. Plant Physiology, 2014, 166(2): 798-807.
[19] Mohammadi M, Soleimani A, Lin Y, et al. Enzymatic depolymerization of lignin and electrocatalytic hydrodeoxygenation for the production of reduced aromatic compounds: a review and perspective[J]. Biotechnology Advances, 2025, 83: 108647.
[20] Das S, Cherwoo L, Singh R. Decoding dye degradation: microbial remediation of textile industry effluents[J]. Biotechnology Notes, 2023, 4: 64-76.
[21] Zhuo R, Fan F. A comprehensive insight into the application of white rot fungi and their lignocellulolytic enzymes in the removal of organic pollutants[J]. Science of the Total Environment, 2021, 778: 146132.
[22] Singh S, Nimse S B, Mathew D E, et al. Microbial melanin: recent advances in biosynthesis, extraction, characterization, and applications[J]. Biotechnology Advances, 2021, 53: 107773.
[23] Kaur K, Sharma A, Capalash N, et al. Multicopper oxidases: biocatalysts in microbial pathogenesis and stress management[J]. Microbiological Research, 2019, 222: 1-13.
[24] Guan Z B, Luo Q, Wang H R, et al. Bacterial laccases: promising biological green tools for industrial applications[J]. Cellular and Molecular Life Sciences, 2018, 75(19): 3569-3592.
[25] 史开篇, 杨洋, 汪娟, 等. 固定化漆酶降解水中酚类污染物研究进展[J]. 南京师范大学学报(工程技术版), 2023, 23(2): 54-61.
[26] 唐禄鑫, 王雅娴, 彭明意, 等. 真菌漆酶及其生产、固定化与应用[J]. 菌物学报, 2023, 42(9): 1821-1837.
[27] 岑庆静, 万辉, 刘薇, 等. 产漆酶真菌筛选、鉴定及酶促去除苯酚的条件优化[J]. 南昌大学学报(理科版), 2022, 46(6): 621-629.
[28] Janusz G, Pawlik A, Świderska-Burek U, et al. Laccase properties, physiological functions, and evolution[J]. International Journal of Molecular Sciences, 2020, 21(3): 966.
[29] 陈中维, 杨锐, 李宁杰, 等. 黄孢原毛平革菌产漆酶优化培养及其对刚果红的脱色降解[J]. 菌物学报, 2021, 40(6): 1538-1548.

备注/Memo

备注/Memo:
基金项目 : 北京市农林科学院创新能力建设项目(KJCX20230102) ;浙江省省产业技术团队项目(浙农科发〔2025〕5号)收稿日期 : 2025-03-04 网络出版日期 : 2026-01-26
作者简介 : 任珂瑶(2000— ) ,女 , 四川乐山人 ,硕士研究生 ,主要从事应用微生物学方面的研究。通信作者: 赵洪新,E-mail:bxxbj2003@gmail. com
更新日期/Last Update: 2026-05-07