|本期目录/Table of Contents|

[1]游慧玉,马蓉,张春妮,等.植物光合作用中铁硫蛋白的功能与调控机制研究进展[J].浙江理工大学学报,2026,55-56(自科三):334-342.
 YOU Huiyu,MA Rong,ZHANG Chuni,et al.Research advances in the function and regulation of iron-sulfur proteins in plant photosynthesis[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科三):334-342.
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植物光合作用中铁硫蛋白的功能与调控机制研究进展()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第三期
页码:
334-342
栏目:
出版日期:
2026-05-10

文章信息/Info

Title:
Research advances in the function and regulation of iron-sulfur proteins in plant photosynthesis
文章编号:
1673-3851(2026) 05-0334-09
作者:
游慧玉马蓉张春妮柯丽萍
浙江理工大学生命科学与医药学院 ,杭州 310018
Author(s):
YOU HuiyuMA RongZHANG ChuniKE Liping
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
铁硫簇 植物 叶绿体 光合作用硫利用因子系统
分类号:
Q945
文献标志码:
A
摘要:
铁硫簇(Fe-S簇)作为铁硫蛋白(Fe-S蛋白)的核心辅因子 ,广泛参与多种关键生物学过程 , 包括光合作用、呼吸作用、电子传递和必需维生素与辅因子的生物合成 。在植物光合系统中 ,光合能量转化效率直接依赖一系列核心电子传递体的结构与功能 ,其中许多关键电子传递体属于 Fe-S蛋白 , 而这些 Fe-S蛋白的功能依赖叶绿体硫利用因子(Sulfurutilizationfactor, SUF) 系统提供成熟的铁硫簇 。该文综述了叶绿体 SUF系统的组装机制、Fe-S蛋白在光合作用中的作用机理 ,并总结了当前关于 Fe-S蛋白在提升作物产量及抗逆性方面的研究进展与应用潜力 ,为提升作物光合效率与抗逆性的遗传改良提供理论依据与分子靶标。

参考文献/References:

[1] Evans J R. Improving photosynthesis[J]. Plant Physiology, 2013, 162(4): 1780-1793.
[2] Croce R, Carmo-Silva E, Cho Y B, et al. Perspectives on improving photosynthesis to increase crop yield[J]. Plant Cell, 2024, 36(10): 3944-3973.
[3] Baslam M, Mitsui T, Hodges M, et al. Photosynthesis in a changing global climate: scaling up and scaling down in crops[J]. Frontiers in Plant Science, 2020, 11: 882.
[4] Pandelia M E, Lanz N D, Booker S J, et al. Mössbauer spectroscopy of Fe/S proteins[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2015, 1853(6): 1395-1405.
[5] Przybyla-Toscano J, Christ L, Keech O, et al. Iron-sulfur proteins in plant mitochondria: roles and maturation[J]. Journal of Experimental Botany, 2021, 72(6): 2014-2044.
[6] Fuss J O, Tsai C L, Ishida J P, et al. Emerging critical roles of Fe-S clusters in DNA replication and repair[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2015, 1853(6): 1253-1271.
[7] 谢涛, 章一帆, 刘云辉, 等. 植物线粒体铁硫簇合成系统及其调控的研究进展[J]. 植物学报, 2025, 60(4): 499-514.
[8] Przybyla-Toscano J, Roland M, Gaymard F, et al. Roles and maturation of iron-sulfur proteins in plastids[J]. Journal of Biological Inorganic Chemistry, 2018, 23(4): 545-566.
[9] Xu X M, Müller S G. Iron-sulfur clusters: biogenesis, molecular mechanisms, and their functional significance[J]. Antioxidants & Redox Signaling, 2011, 15(1): 271-307.
[10] Nachin L, El Hassouni M, Loiseau L, et al. SoxR-dependent response to oxidative stress and virulence of Erwinia chrysanthemi: the key role of SufC, an orphan ABC ATPase[J]. Molecular Microbiology, 2001, 39(4): 960-972.
[11] Ayala-Castro C, Saini A, Outten F W. Fe-S cluster assembly pathways in bacteria[J]. Microbiology and Molecular Biology Reviews, 2008, 72(1): 110-125.
[12] Yang B, Xu C, Cheng Y, et al. Research progress on the biosynthesis and delivery of iron-sulfur clusters in the plastid[J]. Plant Cell Reports, 2023, 42(8): 1255-1264.
[13] Pilon M, Abdel-Ghany S E, Van Hoewyk D, et al. Biogenesis of iron-sulfur cluster proteins in plastids[M]//Genetic Engineering: Principles and Methods. Boston: Springer, 2006, 27: 101-117.
[14] Lu Y. Assembly and transfer of iron-sulfur clusters in the plastid[J]. Frontiers in Plant Science, 2018, 9: 336.
[15] Zhang J, Bai Z, Ouyang M, et al. The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis[J]. EMBO Journal, 2021, 40(13): e106742.
[16] Kairis A, Das Neves B, Couturier J, et al. Iron-sulfur cluster synthesis in plastids by the SUF system: a mechanistic and structural perspective[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2024, 1871(7): 119797.
[17] Hanke G T, Kimata-Ariga Y, Taniguchi I, et al. A post genomic characterization of Arabidopsis ferredoxins[J]. Plant Physiology, 2004, 134(1): 255-264.
[18] Hanke G, Mulo P. Plant type ferredoxins and ferredoxin-dependent metabolism[J]. Plant Cell & Environment, 2013, 36(6): 1071-1084.
[19] Hanke G T, Kurisu G, Kusunoki M, et al. Fd:FNR electron transfer complexes: evolutionary refinement of structural interactions[J]. Photosynthesis Research, 2004, 81(3): 317-327.
[20] Voss I, Goss T, Murozuka E, et al. FdC1, a novel ferredoxin protein capable of alternative electron partitioning, increases in conditions of acceptor limitation at photosystem I[J]. Journal of Biological Chemistry, 2011, 286(1): 50-59.
[21] Simkin A J, McAusland L, Lawson T, et al. Overexpression of the Rieske FeS protein increases electron transport rates and biomass yield[J]. Plant Physiology, 2017, 175(1): 134-145.
[22] Tikhonov A N. The cytochrome b6f complex: biophysical aspects of its functioning in chloroplasts[M]//Membrane Protein Complexes: Structure and Function. Singapore: Springer, 2018: 287-328.
[23] Vassiliev I R, Antonkine M L, Golbeck J H. Iron-sulfur clusters in type I reaction centers[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2001, 1507(1/2/3): 139-160.
[24] Peng L, Yamamoto H, Shikanai T. Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2011, 1807(8): 945-953.
[25] Pan X, Cao D, Xie F, et al. Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase[J]. Nature Communications, 2020, 11: 610.
[26] Takabayashi A, Ishikawa N, Obayashi T, et al. Three novel subunits of Arabidopsis chloroplastic NAD(P)H dehydrogenase identified by bioinformatic and reverse genetic approaches[J]. Plant Journal, 2009, 57(2): 207-219.
[27] Dey D, Tanaka R, Ito H. Structural characterization of the Chlorophyllide a oxygenase (CAO) enzyme through an in silico approach[J]. Journal of Molecular Evolution, 2023, 91(2): 225-235.
[28] Zhao X, Jia T, Hu X. HCAR is a limitation factor for chlorophyll cycle and chlorophyll b degradation in chlorophyll-b-overproducing plants[J]. Biomolecules, 2020, 10(12): 1639.
[29] Kuai B, Chen J, Hörtensteiner S. The biochemistry and molecular biology of chlorophyll breakdown[J]. Journal of Experimental Botany, 2018, 69(4): 751-767.
[30] Hauenstein M, Christ B, Das A, et al. A role for TIC55 as a hydroxylase of phyllobilins, the products of chlorophyll breakdown during plant senescence[J]. Plant Cell, 2016, 28(10): 2510-2527.
[31] Dai S, Friemann R, Glauser D A, et al. Structural snapshots along the reaction pathway of ferredoxin-thioredoxin reductase[J]. Nature, 2007, 448(7149): 92-96.
[32] Ibrahim I M, Wu H, Ezhov R, et al. An evolutionarily conserved iron-sulfur cluster underlies redox sensory function of the chloroplast sensor kinase[J]. Communications Biology, 2020, 3: 13.
[33] Yamori W, Shikanai T. Physiological functions of cyclic electron transport around photosystem I in sustaining photosynthesis and plant growth[J]. Annual Review of Plant Biology, 2016, 67: 81-106.
[34] Peltier G, Aro E M, Shikanai T. NDH-1 and NDH-2 plastoquinone reductases in oxygenic photosynthesis[J]. Annual Review of Plant Biology, 2016, 67: 55-80.
[35] Tombuloglu H, Slimani Y, Tombuloglu G, et al. Engineered magnetic nanoparticles enhance chlorophyll content and growth of barley through the induction of photosystem genes[J]. Environmental Science and Pollution Research, 2020, 27(27): 34311-34321.
[36] Zhang H, Xu Z, Guo K, et al. Toxic effects of heavy metal Cd and Zn on chlorophyll, carotenoid metabolism and photosynthetic function in tobacco leaves revealed by physiological and proteomics analysis[J]. Ecotoxicology and Environmental Safety, 2020, 202: 110856.
[37] Wu J, Hu J, Wang L, et al. Responses of phragmites australis to copper stress: a combined analysis of plant morphology, physiology and proteomics[J]. Plant Biology, 2021, 23(2): 351-362.
[38] Sarewicz M, Szwalec M, Pintscher S, et al. High-resolution cryo-em structures of plant cytochrome b6f at work[J]. Science Advances, 2023, 9(2): eadd9688.
[39] Saroussi S, Redekop P, Karns D A J, et al. Restricting electron flow at cytochrome b6f when downstream electron acceptors are severely limited[J]. Plant Physiology, 2023, 192(2): 789-804.
[40] Cramer W A, Yan J, Zhang H, et al. Structure of the cytochrome b6f complex: new prosthetic groups, Q-space, and the ’hors d’oeuvres hypothesis’ for assembly of the complex[J]. Photosynthesis Research, 2005, 85(1): 133-143.
[41] Kurisu G, Zhang H, Smith J L, et al. Structure of the cytochrome b6f complex of oxygenic photosynthesis: Tuning the cavity[J]. Science, 2003, 302(5647): 1009-1014.
[42] Yamori W, Takahashi S, Makino A, et al. The roles of ATP synthase and the cytochrome b6/f complexes in limiting chloroplast electron transport and determining photosynthetic capacity[J]. Plant Physiology, 2011, 155(2): 956-962.
[43] Price G D, Yu J W, Caemmerer S V, et al. Chloroplast cytochrome b6/f and ATP synthase complexes in tobacco: transformation with antisense RNA against nuclear-encoded transcripts for the Rieske FeS and ATPδ polypeptides[J]. Australian Journal of Plant Physiology, 1995, 22(2): 285-297.
[44] Zhang S, Zou B, Cao P, et al. Structural insights into photosynthetic cyclic electron transport[J]. Molecular Plant, 2023, 16(1): 187-205.
[45] 刘玉凤, 王珍琪, 鹿嘉智, 等. 叶绿体 NAD(P)H 脱氢酶复合体调控光合作用的研究进展[J]. 植物生理学报, 2019, 55(7): 932-940.
[46] Munn-Bosch S, Shikanai T, Asada K. Enhanced ferredoxin-dependent cyclic electron flow around photosystem I and α-tocopherolquinone accumulation in water-stressed ndhB-inactivated tobacco mutants[J]. Planta, 2005, 222(3): 502-511.
[47] Wang P, Duan W, Takabayashi A, et al. Chloroplastic NAD(P)H dehydrogenase in tobacco leaves functions in alleviation of oxidative damage caused by temperature stress[J]. Plant Physiology, 2006, 141(2): 465-474.
[48] Zhang Y, Fan Y, Lv X, et al. Deficiency in NDH-cyclic electron transport retards heat acclimation of photosynthesis in tobacco over day and night shift[J]. Frontiers in Plant Science, 2023, 14: 1267191.
[49] Savitch L V, Ivanov A G, Gudynaite-Savitch L, et al. Cold stress effects on PSI photochemistry in Zea mays: Differential increase of FQR-dependent cyclic electron flow and functional implications[J]. Plant & Cell Physiology, 2011, 52(6): 1042-1054.
[50] Wang F, Yan J, Ahammed G J, et al. PGR5/PGRL1 and NDH mediate far-red light-induced photoprotection in response to chilling stress in tomato[J]. Frontiers in Plant Science, 2020, 11: 669.
[51] Ishikawa N, Takabayashi A, Noguchi K, et al. NDH-mediated cyclic electron flow around photosystem I is crucial for C4 photosynthesis[J]. Plant & Cell Physiology, 2016, 57(10): 2020-2028.
[52] Mochizuki N, Tanaka R, Grimm B, et al. The cell biology of tetrapyrroles: a life and death struggle[J]. Trends in Plant Science, 2010, 15(9): 488-498.
[53] Rüdiger W. Biosynthesis of chlorophyll b and the chlorophyll cycle[J]. Photosynthesis Research, 2002, 74(2): 187-193.
[54] 李佳佳, 于旭东, 蔡泽坪, 等. 高等植物叶绿素生物合成研究进展[J]. 分子植物育种, 2019, 17(18): 6013-6019.
[55] Tanaka R, Tanaka A. Effects of chlorophyllide a oxygenase overexpression on light acclimation in Arabidopsis thaliana[J]. Photosynthesis Research, 2005, 85(3): 327-340.
[56] Biswal A K, Pattanayak G K, Ruhil K, et al. Reduced expression of chlorophyllide a oxygenase (CAO) decreases the metabolic flux for chlorophyll synthesis and downregulates photosynthesis in tobacco plants[J]. Physiology and Molecular Biology of Plants, 2024, 30(1): 1-16.
[57] Wang X, Liu L. Crystal structure and catalytic mechanism of 7-hydroxymethyl chlorophyll a reductase[J]. Journal of Biological Chemistry, 2016, 291(25): 13349-13359.
[58] Piao W, Han S H, Sakuraba Y, et al. Rice 7-hydroxymethyl chlorophyll a reductase is involved in the promotion of chlorophyll degradation and modulates cell death signaling[J]. Molecules and Cells, 2017, 40(10): 773-786.
[59] Hörtensteiner S, Kräutler B. Chlorophyll breakdown in higher plants[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2011, 1807(8): 977-988.
[60] Tanaka A, Ito H. Chlorophyll degradation and its physiological function[J]. Plant & Cell Physiology, 2025, 66(2): 139-152.
[61] Hu X, Gu T, Khan I, et al. Research progress in the interconversion, turnover and degradation of chlorophyll[J]. Cells, 2021, 10(11): 3134.
[62] Boij P, Patel R, Garcia C, et al. In vivo studies on the roles of Tic55-related proteins in chloroplast protein import in Arabidopsis thaliana[J]. Molecular Plant, 2009, 2(6): 1397-1409.
[63] Hirashima M, Tanaka R, Tanaka A. Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana[J]. Plant and Cell Physiology, 2009, 50(4): 719-729.
[64] Dai S, Schwendtmayer C, Schürmann P, et al. Redox signaling in chloroplasts: cleavage of disulfides by an iron-sulfur cluster[J]. Science, 2000, 287(5453): 655-658.
[65] Juniar L, Tanaka H, Yoshida K, et al. Structural basis for thioredoxin isoform-based fine-tuning of ferredoxin-thioredoxin reductase activity[J]. Protein Science, 2020, 29(12): 2538-2545.
[66] Gerken M, Kakorin S, Chibani K, et al. Computational simulation of the reactive oxygen species and redox network in the regulation of chloroplast metabolism[J]. PLoS Computational Biology, 2020, 16(1): e1007102.
[67] Michelet L, Zaffagnini M, Morisse S, et al. Redox regulation of the Calvin-Benson cycle: something old, something new[J]. Frontiers in Plant Science, 2013, 4: 470.
[68] Gurrieri L, Fermani S, Zaffagnini M, et al. Calvin-Benson cycle regulation is getting complex[J]. Trends in Plant Science, 2021, 26(9): 898-912.
[69] Yoshida K, Hisabori T. Divergent protein redox dynamics and their relationship with electron transport efficiency during photosynthesis induction[J]. Plant & Cell Physiology, 2024, 65(5): 737-747.
[70] Yoshida K, Uchikoshi E, Hara S, et al. Thioredoxin-like 2/2-Cys peroxiredoxin redox cascade acts as oxidative activator of glucose-6-phosphate dehydrogenase in chloroplasts[J]. Biochemical Journal, 2019, 476(12): 1781-1790.
[71] Yoshida K, Hisabori T. Biochemical basis for redox regulation of chloroplast-localized phosphofructokinase from Arabidopsis thaliana[J]. Plant & Cell Physiology, 2021, 62(3): 401-410.
[72] Puthiyaveetil S, Kavanagh T A, Cain P, et al. The ancestral symbiont sensor kinase CSK links photosynthesis with gene expression in chloroplasts[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(29): 10061-10066.
[73] Ibrahim I M, Puthiyaveetil S, Khan C, et al. Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts[J]. Photosynthesis Research, 2016, 130(1): 93-101.
[74] He L, Li M, Chen D, et al. Rice ferredoxins localize to chloroplasts/plastids and may function in different tissues[J]. Plant Signaling & Behavior, 2021, 16(9): 1926813.
[75] Chang H, Chen Y T, Huang H E, et al. Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis[J]. Transgenic Research, 2023, 32(6): 547-560.
[76] Ermakova M, Woodford R, Taylor Z, et al. Faster induction of photosynthesis increases biomass and grain yield in glasshouse-grown transgenic Sorghum bicolor overexpressing Rieske FeS[J]. Plant Biotechnology Journal, 2023, 21(6): 1206-1216.
[77] Qiu T, Wei S, Fang K, et al. The atypical dof transcriptional factor OsDes1 contributes to stay-green, grain yield, and disease resistance in rice[J]. Science Advances, 2024, 10(34): eadp0345.
[78] Huang H E, Ho M H, Chang H, et al. Overexpression of plant ferredoxin-like protein promotes salinity tolerance in rice (Oryza sativa)[J]. Plant Physiology and Biochemistry, 2020, 155: 136-146.
[79] Hong C Y, Zheng J L, Chen T Y, et al. PFLP-intensified disease resistance against bacterial soft rot through the MAPK pathway in PAMP-triggered immunity[J]. Phytopathology, 2018, 108(12): 1467-1474.
[80] Namukwaya B, Tripathi L, Tripathi J N, et al. Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease[J]. Transgenic Research, 2012, 21(4): 855-865.
[81] Ger M J, Louh G Y, Lin Y H, et al. Ectopically expressed sweet pepper ferredoxin PFLP enhances disease resistance to Pectobacterium carotovorum subsp. carotovorum affected by harpin and protease-mediated hypersensitive response in Arabidopsis[J]. Molecular Plant Pathology, 2014, 15(9): 892-906.
[82] Wang M, Rui L, Yan H, et al. The major leaf ferredoxin Fd2 regulates plant innate immunity in Arabidopsis[J]. Plant, Cell & Environment, 2018, 19(6): 1377-1390.
[83] Tournaire M D, Scharff L B, Kramer M, et al. Ferredoxin C2 is required for chlorophyll biosynthesis and accumulation of photosynthetic antennae in Arabidopsis[J]. Plant Cell and Environment, 2023, 46(11): 3287-3304.
[84] Nazari M, Kordrostami M, Ghasemi-Soloklui A A, et al. Enhancing photosynthesis and plant productivity through genetic modification[J]. Cells, 2024, 13(16): 1319.
[85] Simkin A J, López-Calcagno P E, Raines C A. Feeding the world: improving photosynthetic efficiency for sustainable crop production[J]. Journal of Experimental Botany, 2019, 70(4): 1119-1140.

备注/Memo

备注/Memo:
基金项目 : 国家自然科学基金项目(32572261, 32170307)础 。光合作用的效率与状态贯穿并调控着植物的整收稿日期 : 2025-12-11 网络出版日期 : 2026-01-27
作者简介 : 游慧玉(2001— ) ,女 ,河南信阳人 ,硕士研究生 ,主要从事植物分子生物学方面的研究。通信作者: 柯丽萍,E-mail:keliping@zstu. edu. cn
更新日期/Last Update: 2026-05-07