|本期目录/Table of Contents|

[1]吴晓奇,杨雪玢,郭建军,等.天麻素通过调控 GSK-3β/P38MAPK通路对过表达 APOE4的SH-SY5Y细胞的保护作用及机制[J].浙江理工大学学报,2026,55-56(自科二):207-218.
 WU Xiaoqi,YANG Xuebin,GUO Jianjun,et al.Protective effect and mechanism of gastrodin on SH-SY5Y cells overexpressingAPOE4by regulating GSK-3/P38 MAPK pathway[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科二):207-218.
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天麻素通过调控 GSK-3β/P38MAPK通路对过表达 APOE4的SH-SY5Y细胞的保护作用及机制()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第二期
页码:
207-218
栏目:
出版日期:
2026-03-15

文章信息/Info

Title:
Protective effect and mechanism of gastrodin on SH-SY5Y cells overexpressingAPOE4by regulating GSK-3/P38 MAPK pathway
文章编号:
1673-3851(2026) 03-0207-12
作者:
吴晓奇 杨雪玢 郭建军 盛清
1. 浙江理工大学生命科学与医药学院 ,杭州 310018;2. 浙江理工大学绍兴生物医药研究院 ,浙江绍兴 312000
Author(s):
WU Xiaoqi YANG Xuebin GUO Jianjun SHENG Qing
1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Shaoxing Academy of Biomedicine, Zhejiang Sci-Tech University, Shaoxing312000, China
关键词:
天麻素阿尔茨海默病载脂蛋白 E4Tau蛋白过度磷酸化糖原合成酶激酶-3β/P38丝裂原活化蛋白激酶细胞凋亡
分类号:
R363;R742
文献标志码:
A
摘要:
为探究天麻素(GAS) 对载脂蛋白E(APOE4) 引发阿尔茨海默病(AD) 的调控作用机制 ,进而为 AD的 干预策略提供新方案 ,该文采用网络药理学方法分析 GAS在 AD 中的潜在作用靶点及相关信号通路 ;通过慢病毒转 染技术构建稳定过表达 APOE4基因的 SH-SY5Y 细胞模型 ,探究 GAS对细胞活力和氧化应激状态的影响 ;利 用 RT-qPCR和 Western blotting技术检测 Bax、Bcl-2、p-Tau(Ser396) 、p-Tau(Thr181) 以及糖原合成酶激酶-3β/P38丝 裂原活化蛋白激酶(GSK-3β、P38MAPK) 等关键蛋白的 mRNA表达水平和蛋白表达水平变化 。结果表明 :GAS可 显著改善因APOE4过表达所导致的SH-SY5Y细胞活力降低和氧化应激损伤 ,抑制细胞凋亡;同时显著抑制 GSK-3β 蛋白的活化 ,下调 p-P38MAPK蛋白的表达水平;并且有效缓解 p-Tau(Ser396) 与 p-Tau(Thr181) 的异常磷酸化及 Aβ蛋白的积聚 。该文研究证实 ,GAS可显著减轻 APOE4过表达引发的氧化应激反应、抑制细胞凋亡 , 通过调控 GSK-3β/P38MAPK信号通路抑制 Tau蛋白的过度磷酸化 ,发挥对神经细胞的保护作用 ,这为 APOE4相关的 AD 防治提供了新的干预策略。

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备注/Memo

备注/Memo:
基金项目 : 浙江理工大学绍兴生物医药研究院开放基金(SXAB202007)收稿日期 : 2025-10-21 网络出版日期 : 2025-12-10
作者简介 : 吴晓奇(1998— ) ,女 ,广东潮州人 ,硕士研究生 ,主要从事生物医药方面的研究。通信作者 : 盛 清 ,E-mail: csheng@zstu. edu. cn
更新日期/Last Update: 2026-03-06