|本期目录/Table of Contents|

[1]林梦君,郭歌,卢冉,等.热激蛋白 70对黄瓜花叶病毒侵染的影响及其调控机制[J].浙江理工大学学报,2026,55-56(自科二):182-189.
　LIN Mengjun,GUO Ge,LU Ran,et al.The effect of heat shock protein70 on cucumber mosaic virus and its regulatory mechanism[J].Journal of Zhejiang Sci-Tech University,2026,55-56(自科二):182-189.
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热激蛋白 70对黄瓜花叶病毒侵染的影响及其调控机制()

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
55-56
期数:
2026年自科第二期
页码:
182-189
栏目:
出版日期:
2026-03-15

文章信息/Info

Title:
The effect of heat shock protein70 on cucumber mosaic virus and its regulatory mechanism
文章编号:
1673-3851(2026) 03-0182-08
作者:
林梦君; 郭歌; 卢冉; 杜志游
1. 浙江理工大学生命科学与医药学院 ,杭州 310018;2. 皖北卫生职业学院医学技术系 ,宿州 234099
Author(s):
LIN Mengjun ; GUO Ge ; LU Ran ; DU Zhiyou
1. College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Department of Medical Technology North Anhui Health Vocational College, Suzhou 234099, China
关键词:
黄瓜花叶病毒; 本氏烟; 热激蛋白 70; 下调表达; 瞬时表达 ; 基因组积累量
分类号:
S432.41
文献标志码:
A
摘要:
真核生物中高度保守的热激蛋白 70(Heatshock protein, HSP70) 参与众多细胞活动 。为明确 HSP70 在黄瓜花叶病毒(Cucumbermosaic virus,CMV) 侵染过程中的作用 ,在 CMV侵染的不同时期 ,通过定量 RT-PCR (Reversetranscription polymerase chain reaction) 检测本氏烟中细胞质定位 HSP70的 mRNA含量 ,分析本氏烟中 HPS70的表达量降低和在本氏烟 HSP70瞬时表达对 CMV症状反应和基因组 RNA积累量的影响 ,并评估 CMV 微型复制子 1a2aR3-gfp在 HSP70瞬时表达本氏烟中绿色荧光表型和基因组RNA的含量 。结果表明:在 CMV侵 染 3、5 d和 7 d的本氏烟中 ,与对照相比 , HSP70的 mRNA含量至少增加 了 1倍 ,在 HSP70瞬时表达本氏烟中 CMV 症状反应加重、病毒含量增加;CMV在 HSP70下调表达本氏烟中症状反应减轻、病毒基因组含量降低;在 HSP70瞬时表达 的 浸 润 叶 中 , 1a2aR3-gfp 的 绿 色 荧 光 表 型、RNA3/RNA4的 积 累 量 和 绿 色荧 光 蛋 白 (Green fluorescenceprotein,GFP)表达水平均明显增加 。该文初步证明了 HSP70通过参与病毒基因组 RNA复制来影响 CMV 症状反应和基因组含量 ,正调控 CMV侵染过程 ,这为进一步解析 CMV与植物互作的分子调控机制提供了理 论依据。

参考文献/References:

[1] Koonin E V, Dolja V V, Krupovic M. The logic of virus evolution [J]. CellHostMicrobe, 2022, 30(7) :917-929.

[2] SinghM K, ShinY, JuS, etal. Heatshock responseand heat shock proteins: Currentunderstanding and future opportunities in human diseases[J]. International Journal of Molecular Sciences, 2024, 25(8) : 4209.

[3] Berka M, Kopeck R, Berkov V et al. Regulation of heat shock proteins70andtheirroleinplantimmunity[J]. Journalof ExperimentalBotany , 2022,73(7) :1894-1909.

[4] YangL, Zhou Y, WangSetal. Noncell-autonomous HSC70.1 chaperone displays homeostatic feedback regulation by binding itsownmRNA [J]. New Phytologist, 2023, 237(6) :2404-2421.

[5] WuS, Zhao Y, Wang D, et al. Mode of action of heat shock protein(HSP) inhibitors againstviruses throughhostHSP and virusinteractions [J]. Genes, 2023, 14(4) : 792.

[6] 梅佳佳 ,赵建元 ,刘倩 ,等. Hsp70在病毒复制中的作用[J]. 病毒 学报 ,2023,39(2) :583-588.

[7] 张蕾 ,张培军 ,郭曦尧 ,等. 热应激蛋白与病毒免疫应答研究进 展[J]. 中国预防兽医学报 ,2020,42(2) :207-211.

[8] Chang H H, LeeC H, Chang C J et al. FKBP-type peptidyl- prolylcis-transisomeraseinteractswiththemovementproteinof tomato leafcurlNew Delhivirusand impactsviralreplication in Nicotianabenthamiana [J]. MolecularPlantPatholology, 2022, 23(4) :561-575.

[9] Alam SB, RochonD. Cucumbernecrosisvirus recruits cellular heatshockprotein 70homologsatseveralstagesofinfection[J]. JournalofVirology , 2015, 90(7) :3302-3317.

[10] NagyP D. Co-opted membranes, lipids, and host proteins: What have we learned from tombusviruses? [J]. Current Opinion in Virology, 2022, 56:101258.

[11] MolhoM, PrasanthKR, PoganyJ etal. Targeting conserved co-opted hostfactorstoblockvirusreplication: Usingallosteric inhibitors of the cytosolic Hsp70s to interfere with tomato bushy stuntvirusreplication[J]. Virology, 2021,563:1-19.

[12] TianS, SongQ, ChengY etal. A viralp3aproteintargetsand inhibitsTaDOF transcriptionfactorstopromotetheexpression ofsusceptibility genes and facilitate viral infection[J]. PLoS Pathogens,2024,20(11) :e1012680.

[13] Kleinow T, HappleA, KoberSetal. Phosphorylationsofthe abutilon mosaic virus movement protein affect its self- Interaction, symptom development, viralDNA accumulation, and hostrange[J]. Frontiersin PlantScience, 2020,11:1155.

[14] Zhao L, Chen Y, Xiao X et al. AGO2a but not AGO2b mediates antiviral defense against inf ection of wild-type cucumbermosaic virus in tomato[J]. Horticulture Research, 2023, 10(5) :uhad043.

[15] ChengY, Zheng T, Yang D, et al. Cucumber mosaic virus impairsthephysiologicalhomeostasisofPanaxnotoginsengand inducessaponin-mediated resistance[J]. Virology, 2024, 591: 109983.

[16] Ham BK, Wang X, Toscano-Morales R etal. Plasmodesmal endoplasmicreticulum proteinsregulateintercellulartrafficking of cucumber mosaic virus in Arabidopsis[J]. Journal of ExperimentalBotany , 2023,74(15) :4401-4414.

[17] Kim M J, Kim H R, Paek K H. Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus1areplication protein[J]. Journal of General Virology, 2006, 87(11) :3425-3431.

[18] HuhSU, Kim M J, Ham B K et al. A zinc finger protein Tsip1 controls cucumber mosaic virus infection by interacting with the replication complex on vacuolar membranes of the tobacco plant[J]. New Phytologist, 2011, 191(3) :746-762.

[19] HaveldaZ, MauleAJ. Complexspatialresponsestocucumber mosaicvirusinfection in susceptibleCucurbita pepo cotyledons [J]. PlantCell, 2000, 12(10) :1975-1986.

[20] ChenZ R, ZhouT, WuXH, etal. Influenceofcytoplasmicheat shock protein 70 on viralinfection of Nicotiana benthamiana[J]. MolecularPlantPathology, 2008,9(6) :809-817.

[21] 王琳 ,程晓东 ,卢冉 ,等. 黄瓜花叶病毒反式遗传复制体系的构 建[J]. 浙江理工大学学报(自然科学版) ,2019,43(2) :262-266.

[22] 史志恒 , 曾嘉琳 ,黄欣如 ,等. 槲皮素及其衍生物抗病毒活性研 究进展[J]. 中国现代应用药学 ,2022,39 (18) :2412-2420.

[23] WangR Y, Stork J, Nagy P D. A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteinsto intracellular membranes[J]. Journal of Virology, 2009, 83(7) :3276-3287.

[24] JiangS, Lu Y, LiK etal. Heatshock protein70isnecessary for rice stripe virus infection in plants[J]. Molecular Plant Pathology, 2014,15(9) :907-1017.

[25] SeoJK, Kwon SJ, ChoiH S et al. Evidence for alternate statesofcucumbermosaicvirus replicase assemblyin positive- and negative-strandRNA synthesis[J]. Virology, 2009, 383 (2) :248-260.

[26] FujikiM, KaczmarczykJ F, YusibovV etal. Developmentof a new cucumber mosaic virus-based plant expression vector with truncated 3amovementprotein[J]. Virology, 2008, 381 (1) :136-142.

[27] RyabovE V, Roberts IM, Palukaitis P et al. Host-specific cell-to-cell and long-distance movements of cucumber mosaic virus are facilitated by the movement protein of groundnut rosettevirus[J]. Virology, 1999,260(1) :98-108.

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备注/Memo

备注/Memo:
基金项目 : 浙江省“领雁”科技攻关计划项目(2023C02018) ; 国家自然科学基金项目(32070154)收稿日期 : 2025-01-22 网络出版日期 : 2025-09-17
作者简介 : 林梦君(2000— ) ,女 ,浙江温州人 ,硕士研究生 ,主要从事植物-病毒互作方面的研究。通信作者 : 杜志游 ,E-mail:Duzy@zstu. edu. cn
更新日期/Last Update: 2026-03-06