|本期目录/Table of Contents|

[1]张伟,韩寅卓,姜永厚.猪萨佩罗病毒聚合酶链式反应检测方法的建立及应用[J].浙江理工大学学报,2024,51-52(自科五):718-724.
 ZHANG Wei,HAN Yinzhuo,JIANG Yonghou.Establishment and application of a polymerase chain reaction detection method for porcine sapelovirus[J].Journal of Zhejiang Sci-Tech University,2024,51-52(自科五):718-724.
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猪萨佩罗病毒聚合酶链式反应检测方法的建立及应用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第51-52卷
期数:
2024年自科第五期
页码:
718-724
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Establishment and application of a polymerase chain reaction detection method for porcine sapelovirus
作者:
张伟韩寅卓姜永厚
浙江理工大学生命科学与医药学院,杭州 310018
Author(s):
ZHANG Wei HAN Yinzhuo JIANG Yonghou
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
猪萨佩罗病毒3D基因聚合酶链式反应特异性灵敏度阳性率
分类号:
TS195-644
文献标志码:
A
摘要:
猪萨佩罗病毒(Porcine sapelovirus,PSV)是引发猪腹泻的重要病毒之一。为开发一种能快速、准确检测PSV的聚合酶链式反应检测方法,针对PSV的保守区3D基因设计了一对特异性引物,优化了聚合酶链式反应退火温度,分析聚合酶链式反应检测方法特异性和灵敏度,并应用于猪场样品检测。结果表明:建立的聚合酶链式反应检测方法可特异性扩增出PSV目的片段,对其他非靶标猪腹泻病毒则无条带产生,检测低限为5拷贝/μL;在浙江地区采集的88个健康猪粪便样本中检测PSV阳性率为7.95%。该PSV聚合酶链式反应检测方法特异性强、灵敏度高,可应用于PSV前期诊断和防控。

参考文献/References:

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10]赵婷婷, 李晶娇, 沈小娟, . 猪萨佩罗病毒研究进展[J. 上海交通大学学报(农业科学版), 2017, 35(6): 62-66.

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备注/Memo

备注/Memo:
收稿日期: 2024-03-27
网络出版日期:2024-06-12
基金项目: 浙江省自然科学基金项目 (LGC21C180002)
作者简介: 张伟(1996—),男,湖南衡阳人,硕士研究生,主要从事动物病毒分子诊断技术的研究
通信作者: 姜永厚,E-mail: yonghoujiang@zstu.edu.cn
更新日期/Last Update: 2024-09-18