|本期目录/Table of Contents|

[1]张秋桠,刘高鹏,刘芹,等.猪流行性腹泻病毒RT-PCR检测方法的建立[J].浙江理工大学学报,2022,47-48(自科四):629-634.
　ZHANG Qiuya,LIU Gaopeng,LIU Qin,et al.Establishment of a RTPCR detection method for porcine epidemic diarrhea virus[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科四):629-634.
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猪流行性腹泻病毒RT-PCR检测方法的建立()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第47-48卷
期数:
2022年自科第四期
页码:
629-634
栏目:
出版日期:
2022-09-30

文章信息/Info

Title:
Establishment of a RTPCR detection method for porcine epidemic diarrhea virus
文章编号:
1673-3851 (2022) 07-0629-06
作者:
张秋桠; 刘高鹏; 刘芹; 姜永厚
浙江理工大学生命科学与医药学院，杭州 310018
Author(s):
ZHANG Qiuya;  LIU Gaopeng;  LIU Qin;  JIANG Yonghou
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
猪流行性腹泻病毒; RTPCR; 检测; 特异性; 灵敏度
分类号:
S854-43；S852-651
文献标志码:
A
摘要:
猪流行性腹泻病毒（Porcine epidemic diarrhea virus，PEDV）是造成猪腹泻的一种主要肠道病毒。为建立一种能快速、准确和适于大批量样品的检测PEDV方法，根据PEDV N蛋白基因保守序列特征设计了一对特异性引物，并对退火温度和循环数等条件进行了优化。结果显示：建立的RTPCR方法特异性地扩增出PEDV目的条带，对非靶标病毒均无交叉反应，最低检测限可达5拷贝/μL；应用该方法对浙江40份健康猪粪便样品进行检测，PEDV检出率为12.5%。结果表明，该文所建立检测PEDV的方法具有较高的特异性和灵敏度，可用于临床诊断和流行性病学调查。

参考文献/References:

［1］Jung K, Saif L J. Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis［J］. The Veterinary Journal, 2015, 204(2): 134-143.

［2］Li W T, van Kuppeveld F J M, He Q, et al. Cellular entry of the porcine epidemic diarrhea virus［J］. Virus Research, 2016, 226: 117-127.

［3］He D, Chen F, Ku X, et al. Establishment and application of a multiplex RTPCR to differentiate wildtype and vaccine strains of porcine epidemic diarrhea virus［J］. Journal of Virological Methods, 2019, 272: 113684.

［4］Wood E N. An apparently new syndrome of porcine epidemic diarrhoea［J］. Veterinary Record, 1977, 100(12): 243-244.

［5］Li W T, Li H, Liu Y B, et al. New variants of porcine epidemic diarrhea virus, China, 2011［J］. Emerging Infectious Diseases, 2012, 18(8): 1350-1353.

［6］Vlasova A N, Marthaler D, Wang Q H, et al. Distinct characteristics and complex evolution of PEDV strains, North America, May 2013February 2014［J］. Emerging Infectious Diseases. 2014, 20(10): 1620-1628.

［7］Wang D, Fang L R, Xiao S B. Porcine epidemic diarrhea in China［J］. Virus Research, 2016, 226: 7-13.

［8］Jung K, Saif L J, Wang Q H. Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and prevention and control［J］. Virus Research, 2020, 286: 198045.

［9］Song D, Park B. Porcine epidemic diarrhoea virus: A comprehensive review of molecular epidemiology, diagnosis, and vaccines［J］. Virus Genes. 2012, 44(2): 167-175.

［10］Zhu Y, Wang G H, Cui Y D, et al. Establishment of a nanoparticleassisted RTPCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus［J］. Archives Virology, 2016, 161(9): 2543-2547.

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备注/Memo

备注/Memo:
收稿日期： 2022-04-11
网络出版日期：2022-06-02
基金项目： 浙江省基础公益研究计划项目 (2018C37051，LGC21C180002)
作者简介： 张秋桠(1996-)，女，江苏南通人，硕士研究生，主要从事病毒分子诊断方面的研究
通信作者： 姜永厚，E-mail: yonghoujiang@zstu.edu.cn
更新日期/Last Update: 2022-09-06