|本期目录/Table of Contents|

[1]郑罗凝,徐增辉,王真,等.基于OptiPrep的循环肿瘤细胞富集体系研究[J].浙江理工大学学报,2015,33-34(自科5):699-705.
　ZHENG Luo ning,XU Zeng hui,WANG Zhen,et al.Study on OptiPrepbased Enrichment System of Circulating Tumor Cell[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):699-705.
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基于OptiPrep的循环肿瘤细胞富集体系研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科5期
页码:
699-705
栏目:
出版日期:
2015-09-10

文章信息/Info

Title:
Study on OptiPrepbased Enrichment System of Circulating Tumor Cell
文章编号:
1673-3851 (2015) 05-0699-07
作者:
郑罗凝;  徐增辉;  王真;  钱其军
1. 浙江理工大学生命科学学院, 杭州 310018; 2. 第二军医大学东方肝胆外科医院, 上海 200438
Author(s):
ZHENG Luoning;  XU Zenghui;  WANG Zhen;  QIAN Qijun
1. School of Life Science, Zhejiang SciTech University, Hangzhou 310018, China;2. Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200438, China
关键词:
OptiPrep分离介质;  密度梯度离心法;  循环肿瘤细胞;  免疫磁珠
分类号:
R7337
文献标志码:
A
摘要:
建立一种简易高效的外周血循环肿瘤细胞(circulating tumor cell, CTC)富集方法。配比不同浓度的OptiPrep溶液梯度离心分离外周血各细胞成分,比较OptiPrep液与现行Ficoll液富集肿瘤细胞的效率、回收率、活性率。以此系统富集肿瘤患者血样中的CTC,结合免疫磁珠筛选、染色体原位荧光杂交和表面抗原免疫荧光技术精确鉴定CTC细胞。结果显示OptiPrep液以1.079 g/mL的密度浓度在1900 r/min离心20 min为最优分离条件。32例临床肿瘤患者血样的CTC检测实验中,均发现有循环肿瘤细胞的存在。结果表明基于OptiPrep液结合免疫磁珠筛选能有效富集外周血循环肿瘤细胞,为实验室常规开展CTC的计数、培养等研究奠定基础。

参考文献/References:

［1］ Williams S C. Circulating tumor cells［J］. Proc Natl Acad Sci U S A, 2013, 110(13): 4861.
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［8］ Mita A, Ricordi C, Messinger S, et al. Antiproinflammatory effects of iodixanol (optiprep)based density gradient purification on human islet preparations［J］. Cell Transplantation, 2010, 19(12): 1537-1546.
［9］ Mita A, Ricordi C, Miki A, et al. Purification method using iodixanol (optiprep)based density gradient significantly reduces cytokine chemokine production from human islet preparations, leading to prolonged betacell survival during pretransplantation culture［J］. Transplantation Proceedings, 2009, 41(1): 314-315.

相似文献/References:

[1]郑罗凝,徐增辉,王真,等.基于OptiPrep的循环肿瘤细胞富集体系研究[J].浙江理工大学学报,2015,33-34(自科4):547.
　ZHENG Luo ning,XU Zeng hui,WANG Zhen,et al.Study on OptiPrepbased Enrichment System of Circulating Tumor Cell[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):547.

备注/Memo

备注/Memo:
收稿日期： 2014-11-24
基金项目： 国家自然科学基金项目(81071850,81301307)
作者简介： 郑罗凝(1986-),女,浙江台州人,硕士研究生,主要从事肿瘤基因治疗方面的研究
通信作者： 钱其军,E-mail:qianqj@163.com
更新日期/Last Update: 2015-09-14