|本期目录/Table of Contents|

[1]吴程雨,叶飘,杨孝婷,等.固定化地衣芽孢杆菌胞外脂肪酶的制备[J].浙江理工大学学报,2015,33-34(自科5):694-698.
　WU Cheng yu,YE Piao,YANG Xiao ting,et al.Preparation of Immobilized Extracellular Lipase from Bacillus Licheniformis[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):694-698.
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固定化地衣芽孢杆菌胞外脂肪酶的制备()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科5期
页码:
694-698
栏目:
出版日期:
2015-09-10

文章信息/Info

Title:
Preparation of Immobilized Extracellular Lipase from Bacillus Licheniformis
文章编号:
1673-3851 (2015) 05-0694-05
作者:
吴程雨;  叶飘;  杨孝婷;  徐叶倩;  巩孝磊;  唐琦清;  赵辅昆;  潘建义;  陈玮
浙江理工大学生命科学学院, 杭州 310018
Author(s):
WU Chengyu;  YE Piao;  YANG Xiaoting;  XU Yeqian;  GONG Xiaolei;  TANG Qiqing; ZHAO Fukun;  PAN Jianyi;  CHEN Wei
School of Life Science, Zhejiang Science and Technology University, Hangzhou 310018, China
关键词:
地衣芽孢杆菌;  脂肪酶;  固定化;  ZHHA
分类号:
Q786
文献标志码:
A
摘要:
脂肪酶是一类重要的水解酶,广泛应用于食品、医药、化工等领域。脂肪酶通过固定化可以提高酶的使用效率。文章采用戊二醛共价连接法将来源于地衣芽孢杆菌(Bacillus licheniformis)的胞外脂肪酶固定于氨基型载体ZHHA之上,将1 g载体置入10 mL酶液中反应3 h后固定化酶活力达到最高为60 U/g湿载体。经固定化后该酶的温度稳定性得到显著提升,当反应温度为45℃时最高酶活力达到220 U/g湿载体。同时,固定化使酶在碱性条件下的稳定性得到提高,最适反应pH值为10。通过自制柱式反应器考察该酶工作稳定性,经过15批连续水解反应,固定化脂肪酶仍保持90％的活力,展现出良好的稳定性。

参考文献/References:

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［13］ 孙健, 周毅频, 陶家权, 等. 巨大芽孢杆菌青霉素G酰化酶在新型环氧载体ZHHA上的固定化［J］. 工业微生物, 2008, 38(1): 1-5.

相似文献/References:

[1]吴程雨,叶飘,杨孝婷,等.固定化地衣芽孢杆菌胞外脂肪酶的制备[J].浙江理工大学学报,2015,33-34(自科4):543.
　WU Cheng yu,YE Piao,YANG Xiao ting,et al.Preparation of Immobilized Extracellular Lipase from Bacillus Licheniformis[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):543.

备注/Memo

备注/Memo:
收稿日期： 2014-11-21
基金项目： 国家自然科学基金项目(31200110);浙江省高校生物学重中之重(一级)学科开放基金项目(2012D11);浙江理工大学科研启动基金项目(13042166-Y)
作者简介： 吴程雨(1990-),男,广西桂林人,硕士研究生,主要从事分子生物学和微生物发酵方面的研究
通信作者： 陈玮,E-mail:cw@zstu.edu.cn
更新日期/Last Update: 2015-09-14