|本期目录/Table of Contents|

[1]韩剑翠,马步云,吴程雨,等.新型CDK抑制剂联合ZD55 MnSOD溶瘤腺病毒体外抑制人结肠癌细胞增殖的研究[J].浙江理工大学学报,2015,33-34(自科5):694-698.
　HAN Jian cui,MA Bu yun,WU Cheng yu,et al.InVitro Inhibitory Effect of Oncolytic Adenovirus ZD55MnSODCombined with New CDK Inhibitor on Human Colon Cancer Cells[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):694-698.
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新型CDK抑制剂联合ZD55 MnSOD溶瘤腺病毒体外抑制人结肠癌细胞增殖的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科5期
页码:
694-698
栏目:
出版日期:
2015-09-10

文章信息/Info

Title:
InVitro Inhibitory Effect of Oncolytic Adenovirus ZD55MnSODCombined with New CDK Inhibitor on Human Colon Cancer Cells
文章编号:
1673-3851 (2015) 05-0694-05
作者:
韩剑翠;  马步云;  吴程雨;  王毅刚;  王世兵
浙江理工大学, a. 新元医学与生物技术研究所; b. 生命科学学院科研实验中心, 杭州 310018
Author(s):
HAN Jiancui;  MA Buyun;  WU Chengyu;  WANG Yigang;  WANG Shibing
a. Xinyuan Institute of Medicine and Biotechnology, b. Research Centre, School of Life Science,Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
溶瘤腺病毒;  SNS032;  结肠癌;  抗癌作用
分类号:
Q786
文献标志码:
A
摘要:
探究携带MnSOD的溶瘤腺病毒AdE1B55KdMnSOD(ZD55MnSOD)联合小分子药物新型CDK抑制剂SNS032对人结肠癌细胞株SW620、HCT116、HT29生长的体外抑制效果,通过溶瘤病毒与药物联合,以期寻求较好的抗癌作用。实验采用MTT法检测5 MOI的ZD55MnSOD与不同剂量的SNS032(0、20、40、80、160 ng/mL)联合处理人结肠癌细胞株,发现病毒与药物联合处理有促进肿瘤细胞凋亡的作用。ZD55MnSOD(5 MOI)与SNS032(80 ng/mL)联合处理SW620、HT29细胞,发现联合作用具有很大程度上的时间依赖性,Hoechst33342染色法和流式细胞仪检测细胞凋亡也显示ZD55MnSOD与SNS032联合处理组的细胞凋亡现象比二者单独使用均具有显著差异。该研究初步证实了ZD55MnSOD与SNS032联合具有互补作用,能有效地在体外抑制人结肠癌细胞的增殖。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2014-11-21
基金项目： 国家自然科学基金项目(31200110);浙江省高校生物学重中之重(一级)学科开放基金项目(2012D11);浙江理工大学科研启动基金项目(13042166-Y)
作者简介： 吴程雨(1990-),男,广西桂林人,硕士研究生,主要从事分子生物学和微生物发酵方面的研究
通信作者： 陈玮,E-mail:cw@zstu.edu.cn
更新日期/Last Update: 2015-09-14