|本期目录/Table of Contents|

[1]赵红玉,张耀洲,于威,等.家蚕表达重组人乳铁蛋白及产物对溃疡性结肠炎的治疗作用[J].浙江理工大学学报,2014,31-32(自科6):716-720.
 ZHAO Hong yu,ZHANG Yao zhou,YU Wei,et al.Therapeutic Effect of Recombinant Human Lactoferrin Expressed in Silkworm and Its Product on Ulcerative Colitis[J].Journal of Zhejiang Sci-Tech University,2014,31-32(自科6):716-720.
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家蚕表达重组人乳铁蛋白及产物对溃疡性结肠炎的治疗作用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第31-32卷
期数:
2014年自科6期
页码:
716-720
栏目:
(自科)生物与生命科学
出版日期:
2014-11-10

文章信息/Info

Title:
Therapeutic Effect of Recombinant Human Lactoferrin Expressed in Silkworm and Its Product on Ulcerative Colitis
文章编号:
1673-3851 (2014) 06-0716-05
作者:
赵红玉 张耀洲 于威 舒特俊 陈剑清
1. 浙江理工大学生命科学学院, 杭州 310018; 2. 天津国际生物医药联合研究院, 天津 300457;3. 天津大学药物科学与研究技术学院,天津 300072
Author(s):
ZHAO Hongyu ZHANG Yaozhou YU Wei CHEN Jianqing SHU Tejun
1. College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2.Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China; 3. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
关键词:
重组人乳铁蛋白 蚕蛹 抑菌活性 口服 溃疡性结肠炎
分类号:
R574.62
文献标志码:
A
摘要:
乳铁蛋白(LF)在抗炎和参与机体免疫调节方面具有重要作用。利用家蚕杆状病毒表达系统构建含人乳铁蛋白( hLF )基因的重组病毒Bm hLF,接种蚕蛹表达重组人乳铁蛋白(rhLF),ELISA法检测rhLF的表达量,体外抑菌试验检测其抑菌活性,并建立小鼠溃疡性结肠炎(UC)模型,探究口服rhLF蚕蛹粉对UC的治疗效果。结果表明:rhLF在蚕蛹感染重组病毒120 h后表达量最高达0.38 mg/mL蚕蛹匀浆上清液;rhLF蚕蛹粉能抑制 E.coli  TG1的生长;口服rhLF蚕蛹粉可明显降低UC小鼠的疾病活动指数(DAI)、髓过氧化物酶(MPO)含量,减轻结肠组织炎症损伤,为今后开发一种新型、廉价的UC口服药物提供药效学实验参考。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2014-01-03
基金项目: 国家高技术发展计划“863”项目(2011AA100603),浙江省自然科学基金面上项目(207217)
作者简介: 赵红玉(1988-),女,河北新乐人,硕士研究生,研究方向为生物工程制药
通信作者: 陈剑清,E-mail:cjqgqj@126.com
更新日期/Last Update: 2014-11-24