|本期目录/Table of Contents|

[1]潘姝花,郑婷婷,郑旭升,等.过表达Akt1细胞株的构建及鉴定[J].浙江理工大学学报,2014,31-32(自科4):462-466.
　PAN Shu hua,ZHENG Ting ting,ZHENG Xu sheng,et al.Establishment and Identification of Over Expression of Akt1 Cell Strain[J].Journal of Zhejiang Sci-Tech University,2014,31-32(自科4):462-466.
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过表达Akt1细胞株的构建及鉴定()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第31-32卷
期数:
2014年自科4期
页码:
462-466
栏目:
(自科)生物与生命科学
出版日期:
2014-07-10

文章信息/Info

Title:
Establishment and Identification of Over Expression of Akt1 Cell Strain
文章编号:
1673-3851 (2014) 04-0462-05
作者:
潘姝花;  郑婷婷;  郑旭升;  吴登伟;  陈培远;  许传莲
浙江理工大学生命科学学院, 杭州 310018
Author(s):
PAN Shuhua;  ZHENG Tingting;  ZHENG Xusheng;  WU Dengwei;  CHEN Peiyuan;  XU Chuanlian
College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
Akt1;  重组质粒;  病毒侵染
分类号:
Q786
文献标志码:
A
摘要:
Akt1是细胞信号传导通路中的关键信号分子,具有促进细胞增殖、生长、迁移、侵袭,以及抑制细胞凋亡,抵抗化疗和放疗等重要作用。文章通过构建pLJM1 Akt1 重组质粒,利用慢病毒侵染的方法将重组质粒转染至K562、Bel 7404细胞,用嘌呤霉素筛选得到Akt1稳定过表达的Bel 7404/Akt1、K562/Akt1细胞株,通过Western bloting分析细胞株中Akt1的表达情况。结果显示:Bel 7404/Akt1与K562/Akt1细胞中Akt1表达量明显高于野生型Bel 7404细胞与K562细胞,成功构建过表达Akt1的K562/Akt1、Bel 7404/Akt1稳转细胞株。Akt1过表达细胞株的成功构建为寻找和筛选高效、低毒、强特异性的Akt1抑制剂以及逆转细胞多药耐药(multidrug resistance, MDR)的研究提供了实验模型。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2013-12-04
作者简介： 潘姝花(1988-),女,浙江富阳人,硕士研究生,主要从事天然产物方面的研究
通信作者： 许传莲,E-mail:chuanlianxu@163.com
更新日期/Last Update: 2014-09-17