|本期目录/Table of Contents|

[1]耿新伟,孙亦灵,张柏超,等.响应面法优化重组毕赤酵母表达纤维素酶EG27的研究[J].浙江理工大学学报,2014,31-32(自科4):456-461.
　GEN Xin wei,SUN Yi lin,ZHANG Bo chao,et al.Study on Expression of Cellulase EG27 through Optimization and Recombination of Pichia Pastoris Based on Response Surface Methodology[J].Journal of Zhejiang Sci-Tech University,2014,31-32(自科4):456-461.
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响应面法优化重组毕赤酵母表达纤维素酶EG27的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第31-32卷
期数:
2014年自科4期
页码:
456-461
栏目:
(自科)生物与生命科学
出版日期:
2014-07-10

文章信息/Info

Title:
Study on Expression of Cellulase EG27 through Optimization and Recombination of Pichia Pastoris Based on Response Surface Methodology
文章编号:
1673-3851 (2014) 04-0456-06
作者:
耿新伟;  孙亦灵;  张柏超;  蒋磊;  陈玮;  赵辅昆
浙江理工大学生命科学学院, 杭州 310018
Author(s):
GEN Xinwei;  SUN Yilin;  ZHANG Bochao;  JIANG Lei;  CHEN Wei;  ZHAO Fukun
School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
纤维素酶;  EG27;  毕赤酵母;  响应面
分类号:
Q786
文献标志码:
A
摘要:
为优化重组毕赤酵母表达动物内源性纤维素酶EG27的培养基条件,在单因素实验基础上,以培养基中酵母粉、蛋白胨、葡萄糖的含量为自变量,EG27的酶活为响应值,采用Box Behnken设计的方法,研究各自变量及其交互作用对毕赤酵母产EG27收率的影响。利用Design Expert 软件和响应面分析相结合的方法对毕赤酵母产动物内源性纤维素酶EG27的培养基条件进行优化,确定了最佳的培养基条件。在该培养条件下,获得最高产酶量为354 U/L。

参考文献/References:

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［7］ Alvira P, TomásPejó E, Ballesteros M, et al. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review［J］. Bioresource Technology, 2010, 101(13): 4851-4861.
［8］ Percival Zhang Y H, Himmel M E, Mielenz J R. Outlook for cellulase improvement: screening and selection strategies［J］. Biotechnology Advances, 2006, 24(5): 452-481.
［9］ Guo R, Ding M, Zhang S, et al. Expression and characterization of two secreted his6 tagged endo beta 1, 4 glucanases from the mollusc ampullaria crossean in pichia pastoris［J］. Acta Biochimica et Biophysica Sinica, 2008, 40(5): 419-425.
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［13］ 田泱源, 李瑞芳. 响应面法在生物过程优化中的应用［J］. 食品工程, 2010(2): 8-11.

相似文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2013-10-30
基金项目： 浙江省大学生科技创新项目(2013R406021)
作者简介： 耿新伟(1989-),男,河南安阳人,硕士研究生,主要从事分子生物学和微生物发酵的研究
通信作者： 陈玮,电子邮箱:cw@zstu.edu.cn
更新日期/Last Update: 2014-09-17