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[1]陈涛,张漫莉,李晶虹,等.响应面法优化重组枯草芽孢杆菌生产纤维素酶EGA的研究[J].浙江理工大学学报,2014,31-32(自科4):451-455.
　CHEN Tao,ZHANG Man li,LI Jing hong,et al.Research on Cellulase EGA Production through Optimization and Recombination of Bacillus Subtilis Based on Response Surface Methodology[J].Journal of Zhejiang Sci-Tech University,2014,31-32(自科4):451-455.
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响应面法优化重组枯草芽孢杆菌生产纤维素酶EGA的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第31-32卷
期数:
2014年自科4期
页码:
451-455
栏目:
(自科)生物与生命科学
出版日期:
2014-07-10

文章信息/Info

Title:
Research on Cellulase EGA Production through Optimization and Recombination of Bacillus Subtilis Based on Response Surface Methodology
文章编号:
1673-3851 (2014) 04-0451-05
作者:
陈涛;  张漫莉;  李晶虹;  吴程雨;  赵辅昆;  陈玮
浙江理工大学生命科学学院, 杭州 310018
Author(s):
CHEN Tao;  ZHANG Manli;  LI Jinghong;  WU Chengyu;  ZHAO Fukun;  CHEN Wei
School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
纤维素酶EGA;  枯草芽孢杆菌;  响应面优化;  发酵
分类号:
Q786
文献标志码:
A
摘要:
通过响应面优化法(response surface methodology,RSM)对分泌表达纤维素酶EGA重组枯草芽孢杆菌的发酵条件进行优化。利用Plackett Burman实验设计对淀粉、酵母粉、蛋白胨等8个因素对枯草芽孢杆菌产纤维素酶EGA的显著影响与否进行评估分析,筛选得到淀粉、酵母粉和MgSO 4·7H 2O为3个显著影响的因素,再利用BoxBehnken实验设计对这3个因素进行进一步优化,确定最佳培养基的配比为淀粉21 g/L,酵母粉11.26 g/L,蛋白胨25 g/L,MgSO 4·7H 2O 1.52 g/L,KH 2PO 4 0.395 g/L,NaCl 3.25 g/L,CaCl 2 0.1 g/L,FeSO 4·7H 2O 0 005 g/L。在最优条件下,利用小型发酵罐对重组菌进行扩大培养,其最高酶活力达到1 264 U/L。

参考文献/References:

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相似文献/References:

[1]仇晶晶,陈玮,丁明,等. 响应面法优化重组枯草芽孢杆菌发酵生产青霉素G酰化酶〖[J].浙江理工大学学报,2012,29(06):868.
　QIU Jing jing,CHEN Wei,DING Ming,et al. Optimization of Penicillin G Acylase Production by RecombinantBacillus Subtilis via Response Surface Analysis[J].Journal of Zhejiang Sci-Tech University,2012,29(自科4):868.

备注/Memo

备注/Memo:
收稿日期： 2013-12-27
基金项目： 浙江省大学生科技创新项目(2013R406021)
作者简介： 陈涛(1988-),男,浙江湖州人,硕士研究生,主要从事分子生物学和微生物发酵的研究
通信作者： 陈玮,电子邮箱:cw@zstu.edu.cn
更新日期/Last Update: 2014-09-17